Wnt-Signaling-Mediated Antiosteoporotic Activity of Porcine Placenta Hydrolysates in Ovariectomized Rats

Ko, Byoung‐Seob; Kim, Da Sol; Kang, Suna; Lee, Na Ra; Ryuk, Jin Ah; Park, Sunmin

doi:10.1155/2012/367698

Cited by 10 publications

(12 citation statements)

References 43 publications

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“…Strong evidence for the essential role of canonical Wnt signaling is derived from the osteoporosis phenotype displayed in Wnt, LRP5 or β-catenin gene knockout mice [43] , [44] , [45] . In line with previous findings [46] , the overall mRNA expressions of Wnt, LRP5 and β-catenin within the tibiae were elevated in OVX rats in the present study, revealing high bone metabolism activities under OVX conditions. The major gene expressions in the canonical Wnt signaling pathway, including Wnt, LRP5 and β-catenin, were all significantly increased after 10-week PEMF treatment, demonstrating the potential activation of Wnt/LRP5/β-catenin signaling pathway by PEMF exposure.…”

Section: Discussionsupporting

confidence: 93%

Pulsed Electromagnetic Fields Improve Bone Microstructure and Strength in Ovariectomized Rats through a Wnt/Lrp5/β-Catenin Signaling-Associated Mechanism

Jing

Jiang

et al. 2013

PLoS ONE

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Growing evidence has demonstrated that pulsed electromagnetic field (PEMF), as an alternative noninvasive method, could promote remarkable in vivo and in vitro osteogenesis. However, the exact mechanism of PEMF on osteopenia/osteoporosis is still poorly understood, which further limits the extensive clinical application of PEMF. In the present study, the efficiency of PEMF on osteoporotic bone microarchitecture and bone quality together with its associated signaling pathway mechanisms was systematically investigated in ovariectomized (OVX) rats. Thirty rats were equally assigned to the Control, OVX and OVX+PEMF groups. The OVX+PEMF group was subjected to daily 8-hour PEMF exposure with 15 Hz, 2.4 mT (peak value). After 10 weeks, the OVX+PEMF group exhibited significantly improved bone mass and bone architecture, evidenced by increased BMD, Tb.N, Tb.Th and BV/TV, and suppressed Tb.Sp and SMI levels in the MicroCT analysis. Three-point bending test suggests that PEMF attenuated the biomechanical strength deterioration of the OVX rat femora, evidenced by increased maximum load and elastic modulus. RT-PCR analysis demonstrated that PEMF exposure significantly promoted the overall gene expressions of Wnt1, LRP5 and β-catenin in the canonical Wnt signaling, but did not exhibit obvious impact on either RANKL or RANK gene expressions. Together, our present findings highlight that PEMF attenuated OVX-induced deterioration of bone microarchitecture and strength in rats by promoting the activation of Wnt/LRP5/β-catenin signaling rather than by inhibiting RANKL-RANK signaling. This study enriches our basic knowledge to the osteogenetic activity of PEMF, and may lead to more efficient and scientific clinical application of PEMF in inhibiting osteopenia/osteoporosis.

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Section: Discussionsupporting

confidence: 93%

Pulsed Electromagnetic Fields Improve Bone Microstructure and Strength in Ovariectomized Rats through a Wnt/Lrp5/β-Catenin Signaling-Associated Mechanism

Jing

Jiang

et al. 2013

PLoS ONE

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“…The significantly higher DKK-1 levels in women in contrast to the male cohort raises the issue of the influence of sex hormones on DKK-1 expression. This is supported by the increased expression of DKK-1 in an animal study with ovariectomized rats and the negative correlation of estrogen and DKK-1 levels in surgically postmenopausal women [22,23]. …”

Section: Discussionmentioning

confidence: 93%

Serum Levels of Sclerostin and Dickkopf-1: Effects of Age, Gender and Fracture Status

Dovjak¹,

Dorfer

Föger-Samwald

et al. 2014

Gerontology

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Background: Fragility fractures, especially hip fractures, are a very common complication of osteoporosis in elderly subjects. Sclerostin (SOST) and dickkopf-1 (DKK-1) are inhibitors of the canonical wnt signalling pathway and thus could be involved in the pathogenesis of age-related bone fragility. Objective: To investigate SOST and DKK-1 in a large group of geriatric patients with hip fractures and to relate the wnt inhibitors to age and gender. Methods: This was a cross-sectional study carried out in a department of acute geriatric care in a district hospital in Upper Austria and a hospital in Vienna, Austria. A total of 256 geriatric patients (172 women and 84 men) and 67 young control subjects were selected after exclusion. Medical history was obtained, a comprehensive geriatric assessment was performed and serum levels of SOST, DKK-1 and bone formation markers were analysed. Results: DKK-1 levels increased with age and in the presence of hip fractures. In contrast, SOST levels were lower in patients with hip fractures. When compared to women, men had higher SOST levels but lower DKK-1 levels. Conclusion: Serum levels of the inhibitors of the canonical wnt signalling pathway reflect different biological events and are useful for the study of bone fragility in geriatric patients.

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“…The respiratory quotients (RQs) and resting energy expenditures (REEs) were calculated using previously reported equations. 22,23 BMD and lean and fat mass measurements A densitometer was calibrated daily with a phantom supplied by the manufacturer. After anesthetization with ketamine and xylazine (100 and 10 mg/kg bw, respectively), the animals were laid in a prone position with their hind legs maintained in external rotation with tape.…”

Section: Energy Expenditure Analysis By Indirect Calorimetrymentioning

confidence: 99%

“…Immunoblotting with specific antibodies against mammalian target of rapamycin (mTOR), eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), phosphorylated mTOR Ser2448 , 4E-BP1 Thr37/46 , and b-actin (Cell Signaling Technology, Beverly, MA) as previously described. 23 The intensity of protein expression was determined using Imagequant TL image analysis software (Amersham Biosciences, Piscataway, NJ).…”

Section: Immunoblot Analysismentioning

confidence: 99%

The supplementation of Korean mistletoe water extracts reduces hot flushes, dyslipidemia, hepatic steatosis, and muscle loss in ovariectomized rats

Kim

Park

Kwon

et al. 2014

Exp Biol Med (Maywood)

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Since Korean mistletoe (Viscum album) has been used for alleviating metabolic diseases, it may also prevent the impairment of energy, glucose, lipid, and bone metabolisms in an estrogen-deficient animal model. We determined that long-term consumption of Korean mistletoe water extract (KME) can alleviate menopausal symptoms such as hot flush, increased abdominal fat mass, dyslipidemia, hyperglycemia, and decreased bone mineral density in ovariectomized (OVX) rats fed a high-fat diet, and explored the mechanisms of the effects. OVX rats were divided into four groups and fed high-fat diets supplemented with either 0.6% dextrin (control), 0.2% lyophilized KME + 0.4% dextrin (KME-L), or 0.6% lyophilized KME (KME-H). Sham rats were fed with the high-fat diets with 0.6% dextrin as a normal-control without estrogen deficiency. After eight weeks, OVX rats exhibited impaired energy, glucose and lipid metabolism, and decreased uterine and bone masses. KME-L did not alleviate energy dysfunction. However, KME-H lowered serum levels of total-, LDL-cholesterol, and triglycerides and elevated serum HDL-cholesterol levels in OVX rats with dyslipidemia, to similar levels as normal-control rats. Furthermore, KME-H improved HOMA-IR, an indicator of insulin resistance, in OVX rats. Surprisingly, KME-H fed rats had greater lean mass in the abdomen and leg without differences in fat mass but neither dosage of KME altered bone mineral density in the lumbar spine and femur. The increased lean mass was related to greater phosphorylation of mTOR and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) in the quadriceps muscles. Hepatic triglyceride contents were lowered with KME-H in OVX rats by increasing carnitine palmitoyltransferase-1 (CPT-1) expression and decreasing fatty acid synthase (FAS) and sterol regulatory element-binding protein-1c (SREBP-1c) expression. In conclusion, KME may be useful for preventing some menopausal symptoms such as hot flushes, dyslipidemia, hepatic steatosis, and loss of muscle mass in post-menopausal women.

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Wnt-Signaling-Mediated Antiosteoporotic Activity of Porcine Placenta Hydrolysates in Ovariectomized Rats

Cited by 10 publications

References 43 publications

Pulsed Electromagnetic Fields Improve Bone Microstructure and Strength in Ovariectomized Rats through a Wnt/Lrp5/β-Catenin Signaling-Associated Mechanism

Pulsed Electromagnetic Fields Improve Bone Microstructure and Strength in Ovariectomized Rats through a Wnt/Lrp5/β-Catenin Signaling-Associated Mechanism

Serum Levels of Sclerostin and Dickkopf-1: Effects of Age, Gender and Fracture Status

The supplementation of Korean mistletoe water extracts reduces hot flushes, dyslipidemia, hepatic steatosis, and muscle loss in ovariectomized rats

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