Wnt signalling in oral and maxillofacial diseases

Zhang, Zhaowei; Pan, Xinyue; Chen, Mingyang; Bai, Mingru

doi:10.1002/cbin.11708

Cited by 6 publications

(2 citation statements)

References 65 publications

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“…Since the Wnt signaling plays an important role in oral and maxillofacial diseases 83 , further clarification of the mechanisms underlying the activities of IL-6 in osteogenic differentiation of hPDLSCs is especially important for management of oral and periodontal diseases as well as tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6

Purwaningrum

Giachelli

Osathanon

et al. 2023

Sci Rep

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Periodontal ligament stem cells (PDLSCs) play a significant role on periodontal tissue and alveolar bone homeostasis. During inflammation, interleukin (IL)-6 serves as one of key cytokine players controlling tissue reaction as well as alveolar bone tissue remodeling. It is believed that periodontal tissue inflammation causes periodontium degradation, especially alveolar bone. However, in this study, we show that an inflammatory mediator, IL-6, may serve another direction on alveolar bone homeostasis during inflammatory condition. We found that, IL-6 at 10 and 20 ng/mL was not cytotoxic and dose-dependently exerted beneficial effects on osteogenic differentiation of human PDLSCs (hPDLSCs), as demonstrated by increased alkaline phosphatase activity, mRNA expression of osteogenic markers, and matrix mineralization. The presence of physiological and inflammatory level of IL-6, the osteogenic differentiation potential by hPDLSCs was enhanced by several possible mechanisms including transforming growth factor (TGF), Wnt, and Notch pathways. After in-depth and thorough exploration, we found that Wnt pathway serves as key regulator controlling osteogenic differentiation by hPDLSCs amid the IL-6 presentation. Surprisingly, apart from other mesenchymal stem cells, distinct Wnt components are employed by hPDLSCs, and both canonical and non-canonical Wnt pathways are triggered by different mechanisms. Further validation by gene silencing, treatment with recombinant Wnt ligands, and β-catenin stabilization/translocation confirmed that IL-6 governed the canonical Wnt/β-catenin pathway via either WNT2B or WNT10B and employed WNT5A to activate the non-canonical Wnt pathway. These findings fulfill the homeostasis pathway governing periodontal tissue and alveolar bone regeneration and may serve for further therapeutic regimen design for restoring the tissues.

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Section: Discussionmentioning

confidence: 99%

Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6

Purwaningrum

Giachelli

Osathanon

et al. 2023

Sci Rep

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“…Wnts belong to a highly conserved family of secreted growth factors, which couple to various receptors, thereby activating different downstream pathways which classify as either canonical or noncanonical signaling pathways (5). The Wnt pathway controls cellular processes, such as proliferation, differentiation and migration (5). Wnt4 is known to regulate noncanonical Wnt signaling (6).…”

Section: Introductionmentioning

confidence: 99%

Wnt4 prevents apoptosis and inflammation of dental pulp cells induced by LPS by inhibiting the IKK/NF‑κB pathway

Wu²,

Miao

et al. 2022

Exp Ther Med

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Wnt4 has been shown to promote the recovery of odontogenic differentiation of dental pulp stem cells under inflammatory conditions, but its role in inflammation and apoptosis of pulpitis remains to be elucidated. Lipopolysaccharide (LPS) (10 µg/ml) was applied to treat the human dental pulp cells (HDPCs) for 24 h. Western blotting measured the expressions of inflammatory cytokines and apoptosis-related proteins. Cell apoptosis was measured by flow cytometry. The level of Wnt4 was evaluated by reverse transcription-quantitative PCR and western blotting. The results indicated that LPS could promote inflammatory response and apoptosis in HDPCs and downregulated Wnt4 expression was found in LPS-HDPCs. Overexpression of Wnt4 ameliorated cell inflammatory response and apoptosis, presented by reduced expressions of IL-8, IL-6, TNF-α, IL-1β, Bax, cleaved-caspase 3 and enhanced Bcl-2 expression as well as decreased apoptosis rate. Moreover, overexpression of Wnt4 reduced the phosphorylation levels of IKK2, IκBα and p65 proteins upregulated by LPS. Finally, overexpression of IKK2 reversed the effects of Wnt4 on inflammation and apoptosis of LPS-HDPCs and NF-κB inhibitor reversed the effect of IKK2 overexpression in LPS-HDPCs. Wnt4 inhibited LPS-triggered inflammation and apoptosis in HDPCs via regulating the IKK/NF-κB signaling pathway, which provided a new viewpoint for understanding the pathological mechanism of pulpitis.

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Regulatory mechanisms and regeneration strategies of the soft–hard tissue interface in the human periodontium

Dang,

Wei,

Wan

et al. 2024

BMEMat

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The soft‐hard tissue interface of the human periodontium is responsible for periodontal homeostasis and is essential for normal oral activities. This soft‐hard tissue interface is formed by the direct insertion of fibrous ligaments into the bone tissue. It differs from the unique four‐layer structure of the fibrocartilage interface. This interface is formed by a combination of physical, chemical, and biological factors. The physiological functions of this interface are regulated by different signaling pathways. The unique structure of this soft‐hard tissue interface has inspired scientists to construct biomimetic gradient structures. These biomimetic systems include nanofiber scaffolds, cell sheets, and hydrogels. Exploring methods to repair this soft‐hard tissue interface can help solve clinically unresolved problems. The present review examines the structure of the soft‐hard tissue interface of the periodontium and the factors that influence the development of this interface. Relevant regulatory pathways and biomimetic reconstruction methods are also presented to provide ideas for future research on interfacial tissue engineering.

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Wnt signalling in oral and maxillofacial diseases

Cited by 6 publications

References 65 publications

Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6

Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6

Wnt4 prevents apoptosis and inflammation of dental pulp cells induced by LPS by inhibiting the IKK/NF‑κB pathway

Regulatory mechanisms and regeneration strategies of the soft–hard tissue interface in the human periodontium

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