WNT5A-Ca2+-CaN-NFAT signalling plays a permissive role during cartilage differentiation in embryonic chick digit development

Farrera-Hernández, Alejandro; Marín-Llera, Jessica Cristina; Chimal‐Monroy, Jesús

doi:10.1016/j.ydbio.2020.10.003

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…5C and D ). CaN promotes nuclear translocation of NFATc1 via dephosphorylation and activates downstream target genes ( 19 ). Therefore, expression of the catalytic subunit of CaN (CNA) was measured in the cytoplasm of PROSER2 shRNA lentivirus-infected MG63 cells using western blotting.…”

Section: Resultsmentioning

confidence: 99%

“…Whether PROSER2 affected the nuclear translocation of NFATc1 was investigated. VIVIT is a specific inhibitor of NFAT and is a cell-permeable peptide that selectively inhibits CaN-mediated dephosphorylation of NFAT ( 19 ). PROSER2 and control shRNA lentivirus MG63 cells were treated with 10 µM VIVIT at 37˚C for 24 h. Nuclear expression of NFATc1, as well as total Wnt5a, COX2, MMP-9 and MMP-2 expression levels, were detected by western blot analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Wnt ligands attract and activate Dishevelled by binding to Frizzled family proteins and further activating phospholipase C. This catalyzes the conversion of phosphatidylinositol 4,5-bisphosphate to 1,4,5-triphosphate inositol and diacylglycerol, leading to release of intracellular calcium ( 18 ). Increased intracellular Ca 2+ activates calcineurin (CaN), Ca 2+ /calmodulin-dependent kinase II and protein kinase C, which stimulate the activation of calcium-related pathway transcription factor and nuclear factor of activated T-cells (NFAT) and regulate transcription and expression of downstream target proteins ( 19 ). It has been reported that the NFATc family is a class of Ca 2+ /CaN-dependent transcription factors, including NFATc1, NFATc2, NFATc3, NFATc4 and NFAT5, which are widely distributed in various human tissue ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

“…Increased intracellular Ca 2+ activates calcineurin (CaN), Ca 2+ /calmodulin-dependent kinase II and protein kinase C, which stimulate the activation of calcium-related pathway transcription factor and nuclear factor of activated T-cells (NFAT) and regulate transcription and expression of downstream target proteins ( 19 ). It has been reported that the NFATc family is a class of Ca 2+ /CaN-dependent transcription factors, including NFATc1, NFATc2, NFATc3, NFATc4 and NFAT5, which are widely distributed in various human tissue ( 19 ). CaN is key for skeletal muscle differentiation and regeneration and conversion of type II skeletal muscle fibers to type I.…”

Section: Introductionmentioning

confidence: 99%

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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

Zhang

et al. 2022

Exp Ther Med

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Proline-and serine-rich 2 (PROSER2) is encoded by the 47th open reading frame on human chromosome 10. Bioinformatic analysis has shown PROSER2 was significantly correlated with prognostic outcome of osteosarcoma patients. Its role in the progression and metastasis of human osteosarcoma has not been elucidated until now. Bioinformatics analysis was performed on 101 patients with osteosarcoma from The Cancer Genome Atlas database. High levels of PROSER2 were associated with a poor prognosis in patients with osteosarcoma. PROSER2 expression was significantly upregulated in clinical specimens from patients with osteosarcoma and osteosarcoma cell lines. MTT assay was performed to test the cell viability and Transwell assay was used to test the migration and invasion of MG63 cells. PROSER2 knockdown inhibited the viability, migration and invasion of MG63 cells. Gene Set Enrichment Analysis and Gene Ontology/Kyoto Encyclopedia of Genes and Genomes analysis showed that the differentially expressed genes were primarily involved in 'calcium signaling pathway' and 'Wnt signaling' in patients with osteosarcoma and high PROSER2 expression. Western blotting analysis revealed that PROSER2 regulated migration and invasion of osteosarcoma via the Wnt/nuclear factor of activated T-cells (NFAT)c1 signaling pathway. In conclusion, PROSER2 promoted the proliferation, migration and invasion of osteosarcoma cells via the Wnt/Ca 2+ /NFATc1 signaling pathway by increasing nuclear localization of NFATc1.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

Zhang

et al. 2022

Exp Ther Med

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“…Wnt5a is a crucial non-canonical Wnt signaling ligand that plays a vital role in the development of musculoskeletal tissues, including cartilage [163]. Farrera-Hernández et al showed that Wnt5a activated calcium release in the undifferentiated region during digit development [164]. Furthermore, they confirmed that the non-canonical Wnt5a-Ca 2+calcineurin (CaN) nuclear factor of activated T-cell (NFAT) signaling pathway played a crucial role during embryonic digit development in vivo, enhancing chondrogenic signaling competence [164].…”

Section: Wingless-type Mmtv Integration Site Family Member 5a (Wnt5a)mentioning

confidence: 99%

Advances in Hydrogels for Meniscus Tissue Engineering: A Focus on Biomaterials, Crosslinking, Therapeutic Additives

Zhou,

Wang,

Jiang

et al. 2024

Gels

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Meniscus tissue engineering has emerged as a promising strategy for meniscus repair and regeneration. As versatile platforms, hydrogels have gained significant attention in this field, as they possess tunable properties that allow them to mimic native extracellular matrices and provide a suitable microenvironment. Additionally, hydrogels can be minimally invasively injected and can be adjusted to match the shape of the implant site. They can conveniently and effectively deliver bioactive additives and demonstrate good compatibility with other functional materials. These inherent qualities have made hydrogel a promising candidate for therapeutic approaches in meniscus repair and regeneration. This article provides a comprehensive review of the advancements made in the research on hydrogel application for meniscus tissue engineering. Firstly, the biomaterials and crosslinking strategies used in the formation of hydrogels are summarized and analyzed. Subsequently, the role of therapeutic additives, including cells, growth factors, and other active products, in facilitating meniscus repair and regeneration is thoroughly discussed. Furthermore, we summarize the key issues for designing hydrogels used in MTE. Finally, we conclude with the current challenges encountered by hydrogel applications and suggest potential solutions for addressing these challenges in the field of MTE. We hope this review provides a resource for researchers and practitioners interested in this field, thereby facilitating the exploration of new design possibilities.

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Curcumin‐activated Wnt5a pathway mediates Ca²⁺ channel opening to affect myoblast differentiation and skeletal muscle regeneration

Wang,

Yang,

et al. 2024

J cachexia sarcopenia muscle

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BackgroundSkeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel.MethodsMyogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining.ResultsDuring myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P < 0.001) and the Wnt5a signalling pathway was activated. Wnt5a overexpression promoted the expression of MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.05), and conversely, knockdown of Wnt5a inhibited their expression (P < 0.001). The Wnt5a pathway mediated the opening of Ca2+ channels, regulated the expression levels of CaN, NFAT2, MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.01) and promoted the differentiation of C2C12 myoblasts and the repair and regeneration of injured skeletal muscle. The expression of Wnt5a, CaN, NFAT2, MyoD, Myogenin, Myf5, and MHC in C2C12 myoblast was significantly increased after curcumin intervention (P < 0.05); however, their expression decreased significantly after knocking down Wnt5a on the basis of curcumin intervention (P < 0.05). Similarly, in Wnt5a knockout mice, the promotion of muscle regeneration by curcumin was significantly attenuated.ConclusionsCurcumin can activate the Wnt5a signalling pathway and mediate the opening of Ca2+ channels to accelerate the myogenic differentiation of C2C12 cells and the repair and regeneration of injured skeletal muscle.

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WNT5A-Ca2+-CaN-NFAT signalling plays a permissive role during cartilage differentiation in embryonic chick digit development

Cited by 7 publications

References 34 publications

PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

Advances in Hydrogels for Meniscus Tissue Engineering: A Focus on Biomaterials, Crosslinking, Therapeutic Additives

Curcumin‐activated Wnt5a pathway mediates Ca²⁺ channel opening to affect myoblast differentiation and skeletal muscle regeneration

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WNT5A-Ca2+-CaN-NFAT signalling plays a permissive role during cartilage differentiation in embryonic chick digit development

Cited by 7 publications

References 34 publications

PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

Advances in Hydrogels for Meniscus Tissue Engineering: A Focus on Biomaterials, Crosslinking, Therapeutic Additives

Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration

Contact Info

Product

Resources

About

Curcumin‐activated Wnt5a pathway mediates Ca²⁺ channel opening to affect myoblast differentiation and skeletal muscle regeneration