Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA

Feike, Ann Caroline; Rachor, Klara; Gentzel, Marc; Schambony, Alexandra

doi:10.1016/j.bbrc.2010.08.074

Cited by 27 publications

(20 citation statements)

References 25 publications

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“…62 The xRor2-mCherry construct is described previously. 63 The pCMV-mMesd construct is described previously. 40 The xCD44-MO (5′-TAACAATCCACAGCATTGAGGCCAT-3′) 43 and standard control-MO (5′-CCTCTTACCTCAGTTACAATTTATA-3′) were obtained from Gene Tools (Philomath, OR, USA).…”

Section: Methodsmentioning

confidence: 99%

CD44 functions in Wnt signaling by regulating LRP6 localization and activation

et al. 2014

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Wnt reception at the membrane is complex and not fully understood. CD44 is a major Wnt target gene in the intestine and is essential for Wnt-induced tumor progression in colorectal cancer. Here we show that CD44 acts as a positive regulator of the Wnt receptor complex. Downregulation of CD44 expression decreases, whereas CD44 overexpression increases Wnt activity in a concentration-dependent manner. Epistasis experiments place CD44 function at the level of the Wnt receptor LRP6. Mechanistically, CD44 physically associates with LRP6 upon Wnt treatment and modulates LRP6 membrane localization. Moreover, CD44 regulates Wnt signaling in the developing brain of Xenopus laevis embryos as shown by a decreased expression of Wnt targets tcf-4 and en-2 in CD44 morphants.

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Section: Methodsmentioning

confidence: 99%

CD44 functions in Wnt signaling by regulating LRP6 localization and activation

et al. 2014

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“…1): (1) Ror2 interacts with Frizzled (Fzd) receptors and the intracellular protein Dishevelled (Dvl) to activate Wnt/PCP signaling, 42,43 (2) Ror2 is able to antagonize Wnt/b-Catenin signaling in cells and embryonic tissues, 44,45 and (3) Ror2 activates a PI3K-dependent signaling cascade that transcriptionally regulates expression of paraxial protocadherin (PAPC). 46,47 PAPC and the closely related PCNS are the two paralogs of Protocadherin 8 (PDCH8) in Xenopus laevis. Notably, PAPC itself acts as a modulator of Wnt/PCP signaling in the dorsal mesoderm.…”

Section: Wnt Signalingmentioning

confidence: 99%

Signaling pathways and tissue interactions in neural plate border formation

Schille

Schambony

2017

Neurogenesis

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The neural crest is a transient cell population that gives rise to various cell types of multiple tissues and organs in the vertebrate embryo. Neural crest cells arise from the neural plate border, a region localized at the lateral borders of the prospective neural plate. Temporally and spatially coordinated interaction with the adjacent tissues, the non-neural ectoderm, the neural plate and the prospective dorsolateral mesoderm, is required for neural plate border specification. Signaling molecules, namely BMP, Wnt and FGF ligands and corresponding antagonists are derived from these tissues and interact to induce the expression of neural plate border specific genes. The present mini-review focuses on the current understanding of how the NPB territory is formed and accentuates the need for coordinated interaction of BMP and Wnt signaling pathways and precise tissue communication that are required for the definition of the prospective NC in the competent ectoderm.

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“…Its role as receptor for Wnt5a and activator of PCP and c-Jun N-terminal kinase (Jnk, also known as Mapk8) signaling is considered one key function of Ror2 that is conserved among vertebrates (Gao et al, 2011;Hikasa et al, 2002;Mikels and Nusse, 2006;Mikels et al, 2009;Nishita et al, 2006;Oishi et al, 2003;Schambony and Wedlich, 2007;Wang et al, 2011;Yamamoto et al, 2008). In addition to activating PCP signaling, in early Xenopus embryos Ror2 signals via an RTK-like pathway involving Shc, phosphatidylinositol-4,5-bisphosphate 3-kinase (Pik3, also known as Pi3k) and Jnk to activate c-Jun and Atf2-dependent transcription of paraxial protocadherin (Papc, also known as Pcdh8) (Feike et al, 2010;Liu et al, 2007Liu et al, , 2008Schambony and Wedlich, 2007). Ror2 is expressed in the neural plate, at the neural plate border, in the neural crest and the neural crest-derived branchial arches in Xenopus laevis, chicken and mouse from late gastrula stages onward (Feike et al, 2010;Hikasa et al, 2002;Matsuda et al, 2001;Stricker et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to activating PCP signaling, in early Xenopus embryos Ror2 signals via an RTK-like pathway involving Shc, phosphatidylinositol-4,5-bisphosphate 3-kinase (Pik3, also known as Pi3k) and Jnk to activate c-Jun and Atf2-dependent transcription of paraxial protocadherin (Papc, also known as Pcdh8) (Feike et al, 2010;Liu et al, 2007Liu et al, , 2008Schambony and Wedlich, 2007). Ror2 is expressed in the neural plate, at the neural plate border, in the neural crest and the neural crest-derived branchial arches in Xenopus laevis, chicken and mouse from late gastrula stages onward (Feike et al, 2010;Hikasa et al, 2002;Matsuda et al, 2001;Stricker et al, 2006). This expression pattern, together with defects in craniofacial development observed in individuals with Robinow syndrome (van Bokhoven et al, 2000) and Ror2 knock-out mice Nomi et al, 2001), indicates a role of Ror2 in neural crest development.…”

Section: Introductionmentioning

confidence: 99%

Ror2 signaling is required for local upregulation of GDF6 and activation of BMP signaling at the neural plate border

et al. 2016

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The receptor tyrosine kinase Ror2 is a major Wnt receptor that activates β-catenin-independent signaling and plays a conserved role in the regulation of convergent extension movements and planar cell polarity in vertebrates. Mutations in the ROR2 gene cause recessive Robinow syndrome in humans, a short-limbed dwarfism associated with craniofacial malformations. Here, we show that Ror2 is required for local upregulation of gdf6 at the neural plate border in Xenopus embryos. Ror2 morphant embryos fail to upregulate neural plate border genes and show defects in the induction of neural crest cell fate. These embryos lack the spatially restricted activation of BMP signaling at the neural plate border at early neurula stages, which is required for neural crest induction. Ror2-dependent planar cell polarity signaling is required in the dorsolateral marginal zone during gastrulation indirectly to upregulate the BMP ligand Gdf6 at the neural plate border and Gdf6 is sufficient to rescue neural plate border specification in Ror2 morphant embryos. Thereby, Ror2 links Wnt/planar cell polarity signaling to BMP signaling in neural plate border specification and neural crest induction.

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Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA

Cited by 27 publications

References 25 publications

CD44 functions in Wnt signaling by regulating LRP6 localization and activation

CD44 functions in Wnt signaling by regulating LRP6 localization and activation

Signaling pathways and tissue interactions in neural plate border formation

Ror2 signaling is required for local upregulation of GDF6 and activation of BMP signaling at the neural plate border

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