Wolbachia detection in Aedes aegypti using MALDI-TOF MS coupled to artificial intelligence

Rakotonirina, Antsa; Caruzzo, Cédric; Ballan, Valentine; Kainiu, Malia; Marin, M.; Colot, Julien; Richard, Vincent; Dupont‐Rouzeyrol, Myrielle; Selmaoui-Folcher, Nazha; Pocquet, Nicolas

doi:10.1038/s41598-021-00888-1

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…This method is faster than the DNA sequencing method. While the result of the identification of the mosquito species can be obtained in about a week for the DNA sequencing method, if sequencing is realized by outside supplier [ 29 ], the MALDI-TOF analysis can be performed in about 2.5 hours with experienced personnel [ 33 ]. In addition, despite the high initial cost of the MALDI-TOF machine, the costs of reagents and consumables required for MALDI-TOF analysis is lower than for DNA sequencing (1 Euro vs 20 Euro per sample, based on experience in New Caledonia) [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species

Rakotonirina

Pol²,

Raharimalala³

et al. 2022

PLoS ONE

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Dengue, Zika and chikungunya viruses cause significant human public health burdens in the world. These arboviruses are transmitted by vector mosquito species notably Aedes aegypti and Aedes albopictus. In the Pacific region, more vector species of arboviruses belonging to the Scutellaris Group are present. Due to the expansion of human travel and international trade, the threat of their dispersal in other world regions is on the rise. Strengthening of entomological surveillance ensuring rapid detection of introduced vector species is therefore required in order to avoid their establishment and the risk of arbovirus outbreaks. This surveillance relies on accurate species identification. The aim of this study was to assess the use of the Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) as a tool for an international identification and surveillance of these mosquito vectors of arboviruses. Field-mosquitoes belonging to 8 species (Ae. aegypti, Ae. albopictus, Aedes polynesiensis, Aedes scutellaris, Aedes pseudoscutellaris, Aedes malayensis, Aedes futunae and Culex quinquefasciatus) from 6 countries in the Pacific, Asian and Madagascar, were included in this study. Analysis provided evidence that a MALDI-TOF database created using mosquitoes from the Pacific region allowed suitable identification of mosquito species from the other regions. This technic was as efficient as the DNA sequencing method in identifying mosquito species. Indeed, with the exception of two Ae. pseudoscutellaris, an exact species identification was obtained for all individual mosquitoes. These findings highlight that the MALDI-TOF MS is a promising tool that could be used for a global comprehensive arbovirus vector surveillance.

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Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species

Rakotonirina

Pol²,

Raharimalala³

et al. 2022

PLoS ONE

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“…Second, cross-sample contamination of DNA may have led to the presence of w AlbA or w AlbB sequencing reads in non-infected samples. Lastly, extremely low density of Wolbachia may lead to screening inaccuracies when densities fall under the limit of detection by qPCR [ 67 , 68 ].…”

Section: Discussionmentioning

confidence: 99%

Sex-specific distribution and classification of Wolbachia infections and mitochondrial DNA haplogroups in Aedes albopictus from the Indo-Pacific

et al. 2022

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The arbovirus vector Aedes albopictus (Asian tiger mosquito) is common throughout the Indo-Pacific region, where most global dengue transmission occurs. We analysed population genomic data and tested for cryptic species in 160 Ae. albopictus sampled from 16 locations across this region. We found no evidence of cryptic Ae. albopictus but found multiple intraspecific COI haplotypes partitioned into groups representing three Asian lineages: East Asia, Southeast Asia and Indonesia. Papua New Guinea (PNG), Vanuatu and Christmas Island shared recent coancestry, and Indonesia and Timor-Leste were likely invaded from East Asia. We used a machine learning trained on morphologically sexed samples to classify sexes using multiple genetic features and then characterized the wAlbA and wAlbB Wolbachia infections in 664 other samples. The wAlbA and wAlbB infections as detected by qPCR showed markedly different patterns in the sexes. For females, most populations had a very high double infection incidence, with 67% being the lowest value (from Timor-Leste). For males, the incidence of double infections ranged from 100% (PNG) to 0% (Vanuatu). Only 6 females were infected solely by the wAlbA infection, while rare uninfected mosquitoes were found in both sexes. The wAlbA and wAlbB densities varied significantly among populations. For mosquitoes from Torres Strait and Vietnam, the wAlbB density was similar in single-infected and superinfected (wAlbA and wAlbB) mosquitoes. There was a positive association between wAlbA and wAlbB infection densities in superinfected Ae. albopictus. Our findings provide no evidence of cryptic species of Ae. albopictus in the region and suggest site-specific factors influencing the incidence of Wolbachia infections and their densities. We also demonstrate the usefulness of ddRAD tag depths as sex-specific mosquito markers. The results provide baseline data for the exploitation of Wolbachia-induced cytoplasmic incompatibility (CI) in dengue control.

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Section: Discussionmentioning

confidence: 99%

Sex-specific distribution and classification ofWolbachiainfections and mitochondrial DNA haplogroups inAedes albopictusfrom the Indo-Pacific

Yang

Chung

Robinson

et al. 2022

Preprint

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The arbovirus vector Aedes albopictus (Asian tiger mosquito) is common throughout the Indo-Pacific region, where most global dengue transmission occurs. We analysed population genomic data and tested for cryptic species in 160 Ae. albopictus sampled from 16 locations across this region. We found no evidence of cryptic Ae. albopictus but found multiple intraspecific COI haplotypes partitioned into groups representing three Asian lineages: East Asia, Southeast Asia and Indonesia. Papua New Guinea (PNG), Vanuatu and Christmas Island shared recent coancestry, and Indonesia and Timor-Leste were likely invaded from East Asia. We used a machine learning trained on morphologically sexed samples to classify sexes using multiple genetic features and then characterized the w AlbA and w AlbB Wolbachia infections in 664 other samples. The w AlbA and w AlbB infections as detected by qPCR showed markedly different patterns in the sexes. For females, most populations had a very high double infection incidence, with 67% being the lowest value (from Timor-Leste). For males, the incidence of double infections ranged from 100% (PNG) to 0% (Vanuatu). Only 6 females were infected solely by the w AlbA infection, while rare uninfected mosquitoes were found in both sexes. The w AlbA and w AlbB densities varied significantly among populations. For mosquitoes from Torres Strait and Vietnam, the w AlbB density was similar in single-infected and superinfected ( w AlbA and w AlbB) mosquitoes. There was a positive association between w AlbA and w AlbB infection densities in superinfected Ae. albopictus . Our findings provide no evidence of cryptic species of Ae. albopictus in the region and suggest site-specific factors influencing the incidence of Wolbachia infections and their densities. We also demonstrate the usefulness of SNPs as sex-specific mosquito markers. The results provide baseline data for the exploitation of Wolbachia -induced cytoplasmic incompatibility (CI) in dengue control.

show abstract

Wolbachia detection in Aedes aegypti using MALDI-TOF MS coupled to artificial intelligence

Cited by 10 publications

References 37 publications

MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species

MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species

Sex-specific distribution and classification of Wolbachia infections and mitochondrial DNA haplogroups in Aedes albopictus from the Indo-Pacific

Sex-specific distribution and classification ofWolbachiainfections and mitochondrial DNA haplogroups inAedes albopictusfrom the Indo-Pacific

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