Wolbachia-induced cytoplasmic incompatibility in Liriomyza trifolii and its possible use as a tool in insect pest control

Tagami, Yohsuke; Doi, Makoto; Sugiyama, Keitaro; Tatara, Akio; Saito, Tsutomu

doi:10.1016/j.biocontrol.2006.03.008

Cited by 18 publications

(22 citation statements)

References 26 publications

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“…Such a cross results in the death of the embryo (Beckmann et al, 2017). This strategy can be used in the mass production and release of incompatible male insects to control wild populations of disease vectors such as the mosquito Culex pipiens (Laven, 1967) and of agricultural pests such as Liriomyza trifolii (Tagami et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Wolbachia induce cytoplasmic incompatibility and affect mate preference in Habrobracon hebetor to increase the chance of its transmission to the next generation

Bagheri

Talebi

Asgari

et al. 2019

Journal of Invertebrate Pathology

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Wolbachia are common intracellular bacteria that are generally found in arthropods, including a high proportion of insects and also some nematodes. This intracellular symbiont can affect sex ratio with a variety of reproductive anomalies in the host, including cytoplasmic incompatibility (CI) in haplodiploids. In this study, we questioned if the parasitoid wasp, Habrobracon hebetor (Hym.: Braconidae), an important biological control agent of many lepidopteran larvae, is infected with Wolbachia. To test this, DNA was extracted from adult insects and subjected to PCR using specific primers to Wolbachia target genes. The results showed a high rate of Wolbachia infection in this parasitoid wasp. To determine the biological function of Wolbachia in H. hebetor, we removed this bacterium from the wasps using antibiotic treatment (cured wasps). Results of crossing experiments revealed that Wolbachia induced CI in H. hebetor in which cured females crossed with infected males produced only males, while both male and female progeny were observed for other crosses. Also, we showed that the presence of Wolbachia in females increased fecundity and female offspring of this parasitoid wasp. The presence of Wolbachia in the males had no significant effect on fecundity and female production, but might have incurred costs. We also investigated the effect of Wolbachia on mate choice and found that Wolbachia affects mating behavior of H. hebetor. Together, we showed that Wolbachia induces CI in H. hebetor and affects host mating behavior in favor of its transmission. Wolbachia utilize these strategies to increase the frequency of infected females in the host population.

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Section: Introductionmentioning

confidence: 99%

Wolbachia induce cytoplasmic incompatibility and affect mate preference in Habrobracon hebetor to increase the chance of its transmission to the next generation

Bagheri

Talebi

Asgari

et al. 2019

Journal of Invertebrate Pathology

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“…Wolbachia are naturally found in up to 60% of all insect species and are propagated through insect populations via reproductive manipulations. In some insect species such as mosquitoes [ 2 , 3 ], tsetse flies[ 4 ] and Liriomyza trifolii [ 5 ] a phenomenon called cytoplasmic incompatibility (CI) mediated by Wolbachia , modifies the sperm of infected males such that crosses with uninfected females do not produce viable offsprings. The effect is rescued in crosses with infected females, leading to a reproductive advantage that favors Wolbachia transmission through the population.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy

et al. 2016

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The release of Wolbachia infected mosquitoes is likely to form a key component of disease control strategies in the near future. We investigated the potential of using near-infrared spectroscopy (NIRS) to simultaneously detect and identify two strains of Wolbachia pipientis (wMelPop and wMel) in male and female laboratory-reared Aedes aegypti mosquitoes. Our aim is to find faster, cheaper alternatives for monitoring those releases than the molecular diagnostic techniques that are currently in use. Our findings indicate that NIRS can differentiate females and males infected with wMelPop from uninfected wild type samples with an accuracy of 96% (N = 299) and 87.5% (N = 377), respectively. Similarly, females and males infected with wMel were differentiated from uninfected wild type samples with accuracies of 92% (N = 352) and 89% (N = 444). NIRS could differentiate wMelPop and wMel transinfected females with an accuracy of 96.6% (N = 442) and males with an accuracy of 84.5% (N = 443). This non-destructive technique is faster than the standard polymerase chain reaction diagnostic techniques. After the purchase of a NIRS spectrometer, the technique requires little sample processing and does not consume any reagents.

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“…The bacteria manipulate the reproduction of their host to ensure their vertical transmission via the death of male eggs (Dyer and Jaenike, 2005), the feminisation of the host (Azzouna et al 2004), thelytokous reproduction (Koivisto and Braig, 2003), or cytoplasmic incompatibility that can cause mating between uninfected females and infected males to result in no diploid offspring (Koivisto and Braig, 2003). Based on this last mode of reproduction, pest management programs using Wolbachia have been developed (Tagami et al, 2006). Arsenophonus is an intracellular symbiotic group of bacteria largely found in arthropods (Novakona et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and genetic diversity of three bacterial endosymbionts (Wolbachia, Arsenophonus, and Rhizobiales) associated with the invasive yellow crazy ant (Anoplolepis gracilipes)

2011

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Invasive species carry pathogens, parasites and mutualistic microorganisms into their new range. We examined the prevalence and genetic diversity of three bacterial endosymbionts (Wolbachia, Arsenophonus, and Rhizobiales) in four Anoplolepis gracilipes (Smith) populations in the Pacific region, four populations from mainland Australia and one population from Christmas Island in the Indian Ocean. Wolbachia was found in eight of the nine sampling sites, with between 31.8 and 100% of ants infected. These infection rates are substantially higher than those previously observed for other invasive ants such as Linepithema humile and Solenopsis invicta. All sequences of Wolbachia were genetically identical. Arsenophonus was observed in five of the nine sampling sites, with infection rates ranging between 4.5 and 50.8%. Like Wolbachia, Arsenophonus can modify the sex-ratio of its hosts via malekilling. Arsenophonus was found to co-occur with Wolbachia in the same ants in five of the nine sampling sites. Rhizobiales is a clade of symbiotic bacteria mostly found in herbivorous ants. These bacteria help provide nitrogen to their hosts and were found in only three of the nine sampling sites with an infection rate of 1.6-11.8%. It also co-occurred with the other bacteria. There was no genetic variation in Arsenophonus and Rhizobiales samples, with the exception of a sequence from one Arsenophonus sample in Samoa that differed by a single base pair. These bacterial endosymbionts may contribute to the population variability in A. gracilipes and may be manipulated for the purpose of pest management.

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Wolbachia-induced cytoplasmic incompatibility in Liriomyza trifolii and its possible use as a tool in insect pest control

Cited by 18 publications

References 26 publications

Wolbachia induce cytoplasmic incompatibility and affect mate preference in Habrobracon hebetor to increase the chance of its transmission to the next generation

Wolbachia induce cytoplasmic incompatibility and affect mate preference in Habrobracon hebetor to increase the chance of its transmission to the next generation

Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy

Prevalence and genetic diversity of three bacterial endosymbionts (Wolbachia, Arsenophonus, and Rhizobiales) associated with the invasive yellow crazy ant (Anoplolepis gracilipes)

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