Wolbachia infection in six species of gall wasps and their parasitoids

Zhao, Gao‐Zhi; Zhu, Teng-Ran; Zeng, Yang; Zhu, Dao‐Hong

doi:10.1016/j.aspen.2020.11.004

Cited by 6 publications

(5 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to true gall formers, the Cynipidae also include phytophagous inquilines, which live inside the galls of other species. Several studies have revealed Wolbachia infection in diverse cynipid species with high infection rates ( Plantard et al, 1998 ; Abe and Miura, 2002 ; Rokas et al, 2002 ; Zhu et al, 2007 ; Yang et al, 2013 ; Hou et al, 2020 ; Zhao et al, 2021 ), and some gall wasp species show multiple Wolbachia infections ( Yang et al, 2013 ; Hou et al, 2020 ). The results of Yang et al (2013) suggested a potential possibility of plant tissue-mediated Wolbachia horizontal transmission between gall inducers and their associated inquilines.…”

Section: Introductionmentioning

confidence: 99%

A Case of Intragenic Recombination Dramatically Impacting the Phage WO Genetic Diversity in Gall Wasps

Zhu

Yang

et al. 2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The phage WO was characterized in Wolbachia, a strictly intracellular bacterium causing several reproductive alterations in its arthropod hosts. This study aimed to screen the presence of Wolbachia and phage WO in 15 gall wasp species from six provinces of southern China to investigate their diversity and prevalence patterns. A high incidence of Wolbachia infection was determined in the gall wasp species, with an infection rate of 86.7% (13/15). Moreover, seven species had double or multiple infections. All Wolbachia-infected gall wasp species were found to harbor phage WO. The gall wasp species infected with a single Wolbachia strain were found to harbor a single phage WO type. On the contrary, almost all species with double or multiple Wolbachia infections harbored a high level of phage WO diversity (ranging from three to 27 types). Six horizontal transfer events of phage WO in Wolbachia were found to be associated with gall wasps, which shared identical orf7 sequences among their respective accomplices. The transfer potentially took place through gall inducers and associated inquilines infected with or without Wolbachia. Furthermore, 10 putative recombination events were identified from Andricus hakonensis and Andricus sp2, which harbored multiple phage WO types, suggesting that intragenic recombination was the important evolutionary force, which effectively promoted the high level of phage WO diversity associated with gall wasps.

show abstract

Section: Introductionmentioning

confidence: 99%

A Case of Intragenic Recombination Dramatically Impacting the Phage WO Genetic Diversity in Gall Wasps

Zhu

Yang

et al. 2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…One explanation for the large number of phage types in A. hakonensis is genetic mutations. The results of Zhu et al (2021) [16] strongly suggest that intragenic recombination was an important evolutionary dynamic promoting a high level of phage WO diversity in gall wasps. Moreover, the present study is novel in providing practical molecular evidence supporting the fact that base deletions are also an important cause of phage WO diversity associated with A. hakonensis.…”

Section: Discussionmentioning

confidence: 89%

“…We collected them from five places in Anhui, Hunan, Zhejiang and Fujian provinces in early May [16]. Several studies have detected high rates of Wolbachia infections in diverse cynipid species [18][19][20][21][22][23][24]. Moreover, some cynipid species are infected with multiple Wolbachia strains [22].…”

Section: Introductionmentioning

confidence: 99%

Design and Testing of Effective Primers for Amplification of the orf7 Gene of Phage WO Associated with Andricus hakonensis

Zhu

Yang

2021

Insects

Self Cite

View full text Add to dashboard Cite

Phage WO was first characterized in Wolbachia, an obligate intracellular Rickettsiales known for its ability to regulate the reproduction of arthropod hosts. In this paper, we focus on the study of virus diversity in Andricus hakonensis and the development of highly effective primers. Based on the existing Wolbachia genome sequence, we designed primers (WO-TF and WO-TR) to amplify the full-length orf7 gene of phage WO. Surprisingly, sequencing results showed a high abundance of other phage WO groups in A. hakonensis, in addition to the four groups previously identified. The results also showed that A. hakonensis contained most of the known types of orf7 genes (I, III, IV, V and VI) and the level of diversity of harbored phage WO was very high. Therefore, we speculated that existing primers were not specific enough and that new primers for the detection of phage WO were needed. Based on the existing orf7 gene sequence, we designed specific detection primers (WO-SUF and WO-SUR). Sequencing results showed that the primers effectively amplified all known types of phage WO. In addition to amplifying most of the known sequences, we also detected some new genotypes in A. hakonensis using the new primers. Importantly, all phage WO groups could be efficiently detected. Combined with the results of previous studies, our results suggest that A. hakonensis contains the largest number of phage types (up to 36 types). This study is novel in that it provides practical molecular evidence supporting base deletions, in addition to gene mutations and genetic recombination, as an important cause of phage WO diversity.

show abstract

“…The Wolbachia surface protein (wsp) has been one of the most widely used for Wolbachia identification and systematics. It is well-known that there are 11 supergroups of Wolbachia which have been designated based on their ftsZ, wsp, and 16S rRNA genes (Riegler et al 2012;Zhao et al 2021). Zhou et al (1998) proposed that the wsp sequence similarity among Wolbachia strains should be greater than 97.5% identical.…”

Section: Discussionmentioning

confidence: 99%

Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host

et al. 2022

View full text Add to dashboard Cite

Abstract. Rovik A, Daniwijaya EW, Supriyati E, Rahayu A, Kumalawati DA, Saraswati U, Handayaningsih AE, Rachman MP, Oktriani R, Kurniasari I, Candrasari DS, Nurhayati I, Sholeh R, Arianto B, Tantowijoyo W, Ahmad RA, Utarini A, Arguni E. 2022. Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host. Biodiversitas 23: 2321-2328. Wolbachia naturally presents in a large number of insects and other arthropod species. The Wolbachia strain wMel from Drosophila melanogaster has been stably transinfected into Aedes aegypti where it stops the mosquito host from being infected with medically important arbovirus like dengue. Consequently, Ae. aegypti infected with wMel have been released in Indonesia as a public health intervention against dengue. This study genetically compared wMel from Yogya field-caught D. melanogaster and the wMel in stably transfected Ae. aegypti used for field releases in Yogyakarta, Indonesia. The genetic similarity between wMel Wolbachia was evaluated by sequencing of Wolbachia surface protein (wsp) gene and some polymorphic genomic regions of insertion sites (IS) and variable number tandem repeats (VNTR) loci. The sequence of the Wolbachia surface protein (wsp) gene was 100% identical between hosts. There is no insertion sequence among specimens. The insertion sequence IS-WD1310 was identical between wMel from both hosts and among other strains, as well as the IS-WD516/7. The VNTR-141 period was identical within wMel from both hosts and among other strains, the VNTR-105 as well. Wolbachia Yogya field-caught D. melanogaster and Wolbachia strain wMel present in Ae. aegypti used for bio-control of dengue were genetically identical. These findings provide beneficial understanding to answer the public attention on safety issues, especially on the genetic similarity between Wolbachia strain in the natural and transfected hosts of this novel technology for dengue control.

show abstract

Wolbachia infection in six species of gall wasps and their parasitoids

Cited by 6 publications

References 49 publications

A Case of Intragenic Recombination Dramatically Impacting the Phage WO Genetic Diversity in Gall Wasps

A Case of Intragenic Recombination Dramatically Impacting the Phage WO Genetic Diversity in Gall Wasps

Design and Testing of Effective Primers for Amplification of the orf7 Gene of Phage WO Associated with Andricus hakonensis

Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host

Contact Info

Product

Resources

About