Energy consumption in greenhouse heating could reach up to 90% of the total energy requirement depending on the type of greenhouse, environmental control equipment and location of the greenhouse. The use of climate conditioning technologies that exploit renewable energy and the application of passive systems to improve the energy efficiency and the sustainability of the greenhouse sector are recommended. During winter 2020-2021, an experimental test was carried out at the University of Bari in a Mediterranean greenhouse heated by a polygeneration system, composed of a solar system and an air-water heat pump. Three localized heating systems were tested to transfer thermal energy close to plants of Roman lettuce. Heating pipes were placed inside the cultivation substrate in the underground pipe system and on the cultivation substrate in the laid pipe system. The third system consists of metal plates heated by steel tubes and placed in the aerial area of plants. A weather climatic station and a sensor system interfaced with a data logger for continuous data acquisition and storage were used. The plate system was the best for air temperature rising, as it allowed an increase of 3.6% compared to the set-up without any localised heating system. The underground pipe system was the best for the soil heating, as it achieved a temperature increase of 92%. Localized soil heating systems contributed significantly to an earlier harvest by almost 2 weeks.