Wood formation during ex vitro acclimatisation in micropropagated true service tree (Sorbus domestica L.)

Ďurkovič, Jaroslav; Misalová, A

doi:10.1007/s11240-008-9492-8

Cited by 21 publications

(15 citation statements)

References 21 publications

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“…Moreover TDZ has been reported to be appropriate in in vitro proliferation step of some prunus spp. [23]. It is worth mentioning that some investigators have reported that TDZ and BA are the cytokinins used the most [12], while Arinaitwe et al [17] and Go´ral-ski et al [18] reported that kinetin and isopentenyl adenine (2iP) have been used less.…”

Section: Introductionmentioning

confidence: 99%

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Arab

Yadollahi

Shojaeiyan

et al. 2014

Journal of Genetic Engineering and Biotechnology

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The objective of this research was to assess the effects of different media i.e. Murashige and Skoog (MS) and Quoirin and Lepoivre (QL), cytokinin type i.e. 6-Benzyladenin (BA) and 6-Benzylaminopurine (BAP) and cytokinin concentration on in vitro proliferation of the G · N15 rootstock. To evaluate the effects of different media and cytokinin type, two separate experiments were conducted as factorial based on completely randomized design, and single nodes were used as explants. The results showed that MS nutrient medium was found to be superior to QL nutrient medium. Regarding the interaction between media and growth regulators, the best interaction was found in MS medium supplemented with 1 mg l À1 BAP resulting in 8.5 new micro shoots/explant while 7.75 shoots were observed in MS medium containing 1.25 mg l À1 BA. The longest length of new microshoots (2.10 cm) was obtained in hormone-free MS medium. Findings of this study showed that there is a significant correlation between the hormone level and plantlet height and formed callus weight so that an increase in BAP and BA levels in both of MS and QL media resulted significantly in height decrease and callus weight increase. The results also suggest that the best and the worst plantlets in terms of quality were observed in hormone-free QL medium and MS medium supplemented with 1.25 mg l À1 , respectively. These results reflect the fact that the presence of high amounts of NH 4 NO 3 and cytokinin especially BAP in culture medium triggered inhibitory effect on shoot growth. ª 2014 Production and hosting by Elsevier B.V. on behalf

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Section: Introductionmentioning

confidence: 99%

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Arab

Yadollahi

Shojaeiyan

et al. 2014

Journal of Genetic Engineering and Biotechnology

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“…Vascular characteristics were determined from SEM images for the sixth annual growth ring (both hybrids) and the seventh annual growth rings (‘Dodoens’ trees only) using the NIS-Elements AR 3.0 image analysis software (Laboratory Imaging, Prague, Czech Republic) as described by Ďurkovič and Mišalová [ 30 ]. The traits such as vessel lumen area ( A ), vessel density per square millimeter of wood ( N ), radial and tangential diameters of vessels were determined for earlywood and latewood separately.…”

Section: Methodsmentioning

confidence: 99%

Different Responses in Vascular Traits between Dutch Elm Hybrids with a Contrasting Tolerance to Dutch Elm Disease

Moravčík

Mamoňová

Račko

et al. 2022

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The ascomycetous fungus Ophiostoma novo-ulmi is the causative agent of the current Dutch elm disease (DED) pandemic, which has ravaged many tens of millions of European and North American elm trees. Host responses in vascular traits were studied in two Dutch elm hybrids, ‘Groeneveld’ and ‘Dodoens’, which show different vascular architecture in the secondary xylem and possess contrasting tolerances to DED. ‘Groeneveld’ trees, sensitive to DED, possessed a high number of small earlywood vessels. However, these trees showed a poor response to DED infection for the earlywood vascular characteristics. Following infection, the proportion of least vessels with a vessel lumen area less than 2500 µm2 decreased from 65.4% down to 53.2%. A delayed response in the increasing density of vessels showing a reduced size in the latewood prevented neither the rapid fungal spread nor the massive colonisation of the secondary xylem tissues resulting in the death of the infected trees. ‘Dodoens’ trees, tolerant to DED, possessed a low number of large earlywood vessels and showed a prominent and fast response to DED infection. Vessel lumen areas of newly formed earlywood vessels were severely reduced together with the vessel size : number ratio. Following infection, the proportion of least vessels with a vessel lumen area less than 2500 µm2 increased from 75.6% up to 92.9%. A trend in the increasing density of vessels showing a reduced size was maintained not only in the latewood that was formed in the year of infection but also in the earlywood that was formed in the consecutive year. The occurrence of fungal hyphae in the earlywood vessels that were formed a year following the infection was severely restricted, as revealed by X-ray micro-computed tomography imaging. Possible reasons responsible for a contrasting survival of ‘Groeneveld’ and ‘Dodoens’ trees are discussed.

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“…(), rinsed with water, dehydrated in an ascending ethanol series, rinsed with xylene, mounted in a drop of Clarion mounting medium beneath a coverslip, and examined immediately with an Olympus BX50F microscope (Olympus Europa, Hamburg, Germany). Quantitative data of vascular traits (vessel density per unit area and vessel lumen area per the entire growth ring) were determined with NIS‐Elements AR 3.0 image analysis software (Laboratory Imaging, Prague, Czech Republic) as described in Ďurkovič & Mišalová (). Mean theoretical relative conductivity (RC) was calculated according to Tyree & Zimmermann () as the fourth power of the equivalent circle diameter of the vessel lumen.…”

Section: Methodsmentioning

confidence: 99%

Seasonal patterns of leaf photosynthetic and secondary xylem vascular traits in current‐year stems of three Sorbus species with contrasting growth habits

et al. 2014

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Seasonal effects of environmental variables on photosynthetic activity and secondary xylem formation provide data to demonstrate how environmental factors together with leaf ageing during the season control tree growth. In this study, we assessed physiological responses in photosynthetic behaviour to seasonal climate changes, and also identified seasonal differences in vascular traits within differentiating secondary xylem tissue from three diploid species of the taxonomically complex genus Sorbus. From sampling day 150, a clear physiological segregation of S. chamaemespilus from S. torminalis and S. aria was evident. The shrubby species S. chamaemespilus could be distinguished by a higher photosynthetic capacity between days 150 and 206. This was reflected in its associations with net CO2 assimilation rate (PN), maximum photochemical efficiency of PSII (F(v)/F(m)), variable-to-initial fluorescence ratio (F(v)/F(0)), potential electron acceptor capacity ('area') in multivariate space, and also its associations with log-transformed vessel area and log-transformed relative conductivity between days 239 and 268. The maximum segregation and differentiation among the examined Sorbus species was on sampling day 206. The largest differences between S. torminalis and S. aria were found on day 115, when the latter species clearly showed closer associations with high values of vessel density and transpiration (E). Sampling day clusters were arranged along an arch-like gradient that reflected the positioning of the entire growing season in multivariate space. This arch-like pattern was most apparent in the case of S. chamaemespilus, but was also observed in S. torminalis and S. aria.

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Wood formation during ex vitro acclimatisation in micropropagated true service tree (Sorbus domestica L.)

Abstract: Micropropagated plantlets derived from a superior 90?-year-old slow-growing true service tree (Sorbus domestica L.

Cited by 21 publications

References 21 publications

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Different Responses in Vascular Traits between Dutch Elm Hybrids with a Contrasting Tolerance to Dutch Elm Disease

Seasonal patterns of leaf photosynthetic and secondary xylem vascular traits in current‐year stems of three Sorbus species with contrasting growth habits

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