The objective of this study was to evaluate the composition of OSB panels created using the Amazonian species Caryocar villosum Aubl., Erisma uncinatum Warm., and Hymenolobium excelsum Ducke. The OSB was produced using a phenol–formaldehyde adhesive at a ratio of 30:40:30 for each layer. Different mixtures of Amazonian wood and Pinus caribaea var. caribaea. were tested. Physical (water absorption and thickness swelling after 2 and 24 h of immersion in water and non-return rate in thickness) and mechanical (internal bond and parallel and perpendicular static bending) tests were performed. The OSB panels composed of wood mixtures absorbed less water after 2 and 24 h of immersion when compared to the OSB produced using pine wood. After 24 h of immersion in water, the T3 panel (40% Pinus caribaea + 40% Hymenolobium excelsum + 20% Caryocar villosum) presented the lowest absorption value. In terms of swelling in thickness at 2 h and 24 h, the panel composed of 100% pine strands showed the highest swelling value, while the T3 treatment exhibited the lowest swelling value at 2 and 24 h, as well as for the rate of non-return in thickness. Regarding mechanical properties, we observed that all panels exhibited similar resistance for both parallel and perpendicular Modulus of Rupture, as well as parallel and perpendicular Modulus of Elasticity, except for the internal bond, where T3 demonstrated the highest resistance. Additionally, some OSB panels met the requirements of the EN 300 standard for OSB panels.