Introduction: Healthcare providers are at high risk of occupational stress, burnout, health hazards and job dissatisfaction which can have harmful consequences on their health, work ability and professional efficiency. Therefore, it is important to adopt strategies for the promotion of healthy working conditions and improvement of their work ability. Aim of work: To assess the perceived work ability and its dimensions among the healthcare providers at Minia city, Egypt and identifies its relationship with socio-demographics, work-related factors and chronic diseases. Materials and methods: In a cross-sectional study among 233 healthcare providers, data about sociodemographics, history of chronic diseases, work-related factors and the Work Ability Index (WAI) questionnaire were collected during an interview with the participants. Multinomial logistic regression analyses were performed to identify the determinants of WAI. Results: The study included 145 (62.2%) nurses, 46 (19.8%) physicians and 42 (18%) technicians. The mean WAI score was 40.6 (± 4.6) and the participants were categorized based on their work ability into poor (14.1%), suboptimal (58.4%) and optimal (27.5%). There were significant differences in the WAI scores and most dimensions of work ability between different age groups. In the final multivariable analysis: age, sex, work category and chronic disorders (including musculoskeletal, cardiovascular, respiratory and gastrointestinal) were the significant predictors of work ability among the studied healthcare providers. Conclusion: Low work ability among health care providers was significantly associated with age, sex, work category, and chronic disease conditions. Therefore, promoting health, managing and preventing chronic diseases particularly at old age is essential in designing effective interventions to maintain and improve the work ability of healthcare providers.