Background: The World Health Organization (WHO) and International Labour Organization (ILO) have identified musculoskeletal disorders (MSDs) as a rising epidemic within the workforce. These injuries account for 33% of all occupational injuries and 40% of medical costs. Objectives: This study aimed to determine the effect of MSDs on the job performance of operating room employees. Methods: This descriptive and analytical study was conducted on 100 participants. Job performance was assessed using the Patterson Job Performance Questionnaire (JPQ), and MSDs were evaluated using the NORDIC Questionnaire. Data were analyzed with SPSS version 16, employing Mann-Whitney, chi-square, and binary logistic regression tests. Results: Among the participants, 43% were women, and 67% were men, with an average age of 27.37 ± 6.45. Sixty-two percent of participants did not have acceptable job performance. Additionally, 83% reported at least one musculoskeletal injury, 64% had at least two injuries, and 62% had three or more injuries across different body parts. The results indicated a significant relationship between job performance and variables such as hip, knee, lower back, upper back injuries, age, gender, literacy level, physical activity, and work experience. However, no significant relationship was found between neck injuries and marital status with job performance (P < 0.05). Conclusions: This study demonstrates that MSDs can negatively impact employee job performance. Given the influence of various occupational factors on performance, it is recommended that future studies simultaneously examine the effects of other occupational hazards.