The present study aims to translate the Family-Supportive Coworker Behavior (FSCB) Scale into Chinese version (FSCB-C) and examine its reliability and validity through two studies. In Study 1 ( N = 392), the exploratory factor analysis was conducted, which confirmed that the two-factor structure was consistent with the original scale. In Study 2 ( N = 374), two-wave data was collected for confirmatory factor analysis and the results showed that the two-factor model was a good fit. Furthermore, the measurement invariance results displayed that the FSCB-C was equivalent across gender, age, education level, and job tenure. Additionally, the convergent validity and predictive validity were also demonstrated. The results showed that the FSCB-C could significantly predict job satisfaction, altruism towards colleagues, job stress, work-family conflict, and turnover intention. These findings provide evidence that the Chinese version of the FSCB scale is a valuable instrument.