Workers exposed to wood dust have an increased micronucleus frequency in nasal and buccal cells: results from a pilot study

Bruschweiler, Evin Danisman; Hopf, Nancy B.; Wild, Pascal; Huynh, Cong Khanh; Fenech, Michael; Thomas, Philip; Hor, Maryam; Charrière, Nicole; Savova-Bianchi, Dessislava; Danuser, Brigitta

doi:10.1093/mutage/geu003

Cited by 27 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings are in line with previous studies that report increased biomarkers in various tissues, such as peripheral blood and oral cells, of workers exposed to wood dust by micronucleus and comet (single-cell gel electrophoresis) techniques [28,29,30]. In those studies, high DNA strand breaks [28,29,30] and enhanced chromosomal instability values [2,28,33] were found in the woodworkers relative to the unexposed controls, but discrepant findings were also shown [34]. Results also provide evidence of a significant M 1 dG increment (147% excess) in the long-term workers compared to those with shorter exposure, used as a reference level.…”

Section: Discussionsupporting

confidence: 93%

A Cross-Sectional Study on 3-(2-Deoxy-β-D-Erythro-Pentafuranosyl)Pyrimido[1,2-α]Purin-10(3H)-One Deoxyguanosine Adducts among Woodworkers in Tuscany, Italy

Cellai

Capacci²,

Sgarrella³

et al. 2019

IJMS

View full text Add to dashboard Cite

Occupational exposure to wood dust has been estimated to affect 3.6 million workers within the European Union (EU). The most serious health effect caused by wood dust is the nasal and sinonasal cancer (SNC), which has been observed predominantly among woodworkers. Free radicals produced by inflammatory reactions as a consequence of wood dust could play a major role in SNC development. Therefore, we investigated the association between wood dust and oxidative DNA damage in the cells of nasal epithelia, the target site of SNC. We have analyzed oxidative DNA damage by determining the levels of 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine (M1dG), a major-peroxidation-derived DNA adduct and a biomarker of cancer risk in 136 woodworkers compared to 87 controls in Tuscany, Italy. We then examined the association of M1dG with co-exposure to volatile organic compounds (VOCs), exposure length, and urinary 15-F2t isoprostane (15-F2t-IsoP), a biomarker of oxidant status. Wood dust at the workplace was estimated by the Information System for Recording Occupational Exposures to Carcinogens. M1dG was measured using 32P-postlabeling and mass spectrometry. 15-F2t-IsoP was analyzed using ELISA. Results show a significant excess of M1dG in the woodworkers exposed to average levels of 1.48 mg/m3 relative to the controls. The overall mean ratio (MR) between the woodworkers and the controls was 1.28 (95% C.I. 1.03–1.58). After stratification for smoking habits and occupational status (exposure to wood dust alone and co-exposure to VOCs), the association of M1dG with wood dust (alone) was even greater in non-smokers workers, MR of 1.43 (95% C.I. 1.09–1.87). Conversely, not consistent results were found in ex-smokers and current smokers. M1dG was significantly associated with co-exposure to VOCs, MR of 1.95 (95% C.I. 1.46–2.61), and occupational history, MR of 2.47 (95% C.I. 1.67–3.62). Next, the frequency of M1dG was significantly correlated to the urinary excretion of 15-F2t-IsoP, regression coefficient (β) = 0.442 ± 0.172 (SE). Consistent with the hypothesis of a genotoxic mechanism, we observed an enhanced frequency of M1dG adducts in woodworkers, even at the external levels below the regulatory limit. Our data implement the understanding of SNC and could be useful for the management of the adverse effects caused by this carcinogen.

show abstract

Section: Discussionsupporting

confidence: 93%

A Cross-Sectional Study on 3-(2-Deoxy-β-D-Erythro-Pentafuranosyl)Pyrimido[1,2-α]Purin-10(3H)-One Deoxyguanosine Adducts among Woodworkers in Tuscany, Italy

Cellai

Capacci²,

Sgarrella³

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…In previous observational cohort studies, male woodworkers exposed to external wood-containing particles had a significantly increased risk of oral and pharyngeal cancer among (SIR = 2.19, 95% CI: 1.17-3.74), and the risk of buccal cavity cancer was even higher than that of pharyngeal cancer 32 . At a cellular level, EAA exposure appears to elevate the risk of chromosomal instability in buccal cells by increasing the frequency of micronuclei, which may induce abnormal cell proliferation or apoptosis 33 . The research has also demonstrated the highest risks of cancer with EAA exposure in the lip, oral cavity, and pharynx.…”

Section: Discussionmentioning

confidence: 99%

External Airborne-agent Exposure Increase Risk of Digestive Tract Cancer

Lee

Kim

Lim

et al. 2020

Sci Rep

View full text Add to dashboard Cite

previous studies have suggested that in addition to respiratory system cancers, exposure to external airborne agents (EAAs) may also affect the risk of digestive tract cancer. However, previous epidemiological studies have been limited. To clarify this relationship, we conducted a Workers' Korea National Health Insurance Service cohort study. The EAA exposure group comprised participants who had ever visited a hospital as an inpatient for 'lung diseases due to external agents'. The reference population comprised men from the general working population. The EAA exposure group and reference group included a total of 98,666 and 79,959,286 person-years, respectively. Age-adjusted standardized incident rates (SIRs) with 95% confidence intervals (95%CI) were calculated for each 5-year age stratum. The SIR (95% CI) of EAA exposure was 1.30 (1.19-1.38) for all digestive tract cancers. The highest risk associated with eAA exposure was observed for oral cancer, followed by esophageal and stomach cancers [SIRs (95%CI): 3.96 (3.02-4.78), 3.47(2.60-4.25), and 1.34(1.17-1.47), respectively.] These statistically significant associations did not be attenuated in a subgroup analysis using logistic regression adjusted for age, smoking and alcohol consumption. Our findings suggest that EAA exposure should address risk reduction of both digestive tract and respiratory system cancers. Previous studies of the human health effects of external airborne agent (EAA) which were included aerosol, gas, vapor, mist, fume, dust, or smoke exposure have focused on the respiratory system 1-3 , given the assumption that such exposure primarily causes respiratory system disease. Although EAA exposure mainly occurs via the respiratory system, humans face other possible sites of exposure, namely the digestive system. The digestive system may be exposed to EAAs via several inhalation and ingestion mechanisms. First, agents filtered in the nose or exhaled from the upper respiratory tract may be swallowed 4. Second,, reduced sphincter tone around the esophageal orifice could directly allow the accidental swallowing of EAAs 5. Third, food, skin, or clothing may be contaminated by EAAs 4. Finally, an intuitive approach reveals that the origins of the gastrointestinal tract and respiratory system occur in shared structures, such as the oral cavity and pharynx. In summary, these mechanisms identify the gastrointestinal tract as a potential site of EAA exposure, with the potential for inflammatory, immunologic, or even oncogenic responses similar to those observed in the respiratory system 6. To date, little is known about the relationship between EAA exposure and the risk of digestive tract cancers. Notably, a previous well-designed cohort study found that esophageal and stomach cancer were more closely correlated with EAA exposure when compared with lung cancer, although the authors did not control for alcohol consumption 7. Nevertheless, these results encouraged subsequent epidemiological studies. Although later research also demonstrated the effects of E...

show abstract

“…wood dust, PAHs, and baP exposure assessment. Personal inhalable dust concentrations were determined for two consecutive work shifts for all exposed workers as described in the study by Bruschweiler et al 5 The wood dust was collected on a 37 mm closed-face cassette sampler equipped with glass fiber filters (GF/B, Ø37 mm, Whatman) that operated with a flow rate of 2 L/minute (Esscort ELF pump; MSA). Wood dust concentrations were determined by gravimetric analysis.…”

Section: Methodsmentioning

confidence: 99%

“…To reduce the risk of cancer, it is, therefore, necessary to find better ways of prevention, early diagnosis, and treatment of wood dustrelated diseases. 2 The genotoxic effects of wood dust among exposed workers have been assessed using biomarkers of effects, such as the micronucleus (MN) assay in blood, 3 buccal, 4,5 and nasal cells. 5 Although the genotoxic effects of wood dust have been known for some time, only two studies 3,6 have measured DNA damage using the comet assay among wood workers.…”

Section: Introductionmentioning

confidence: 99%

DNA Damage among Wood Workers Assessed with the Comet Assay

Bruschweiler

Wild

Huynh

et al. 2016

Environ�Health�Insights

Self Cite

View full text Add to dashboard Cite

Exposure to wood dust, a human carcinogen, is common in wood-related industries, and millions of workers are occupationally exposed to wood dust worldwide. The comet assay is a rapid, simple, and sensitive method for determining DNA damage. The objective of this study was to investigate the DNA damage associated with occupational exposure to wood dust using the comet assay (peripheral blood samples) among nonsmoking wood workers (n = 31, furniture and construction workers) and controls (n = 19). DNA damage was greater in the group exposed to composite wood products compared to the group exposed to natural woods and controls (P < 0.001). No difference in DNA damage was observed between workers exposed to natural woods and controls (P = 0.13). Duration of exposure and current dust concentrations had no effect on DNA damage. In future studies, workers’ exposures should include cumulative dust concentrations and exposures originating from the binders used in composite wood products.

show abstract

Workers exposed to wood dust have an increased micronucleus frequency in nasal and buccal cells: results from a pilot study

Cited by 27 publications

References 35 publications

A Cross-Sectional Study on 3-(2-Deoxy-β-D-Erythro-Pentafuranosyl)Pyrimido[1,2-α]Purin-10(3H)-One Deoxyguanosine Adducts among Woodworkers in Tuscany, Italy

A Cross-Sectional Study on 3-(2-Deoxy-β-D-Erythro-Pentafuranosyl)Pyrimido[1,2-α]Purin-10(3H)-One Deoxyguanosine Adducts among Woodworkers in Tuscany, Italy

External Airborne-agent Exposure Increase Risk of Digestive Tract Cancer

DNA Damage among Wood Workers Assessed with the Comet Assay

Contact Info

Product

Resources

About