The output performance of piezoelectric pumps is not only affected by the structural design but is also related to the drive signal. To study the effect of different drive signals on the output performance of piezoelectric pumps, this paper takes dual-chamber serial piezoelectric pumps as the investigation object, theoretically deduces the effective value of the drive signal and the output performance of the piezoelectric pump, and tests the displacement of piezoelectric vibrator center, the output performance of the piezoelectric pump, and the operating noise within the range of 0–500 Hz, respectively, driven by square waves, sine waves, and triangular waves (the peak-to-peak values of which are all 300 V). The results show that at low frequencies, the piezoelectric vibrator’s center displacement curve matches the drive signal, which is sinusoidal and decreases with frequency. Under the square drive, the piezoelectric pump has the best performance, with a flow of 147.199 mL/min and pressure of 14.42 kPa, but the noise is also the highest. The output performance of the sine wave is better than that of the triangular wave, and the flow rate of the three signals shows a trend of first increasing and then decreasing.