International expeditions extensively excavated Lower Nubia (between the First and Second Nile Cataracts) before it was submerged under the waters of Lake Nasser and Lake Nubia. The expeditions concentrated on monumental architecture and cemeteries, including sites at Qustul and Serra East, where the New Kingdom, and Napatan, Meroitic, Nobadian, and Makurian-period elites and common people were buried, ca. 1400 BC–AD 1400. Although the finds abound in adornments, including bead imports from Egypt and South India/Sri Lanka, only a few traces of local glass bead-making have been recorded in Nubia so far. Based on results of laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) analysis of 76 glass beads, pendants, and chunks from Qustul and Serra East contexts, dated between the New Kingdom and the Makuria Kingdom periods, this paper discusses the composition and provenance of two types of plant-ash soda-lime (v-Na-Ca) glass, two types of mineral soda-lime glass (m-Na-Ca), and two types of mineral-soda-high alumina (m-Na-Al) glass. It also presents the remains of a probable local glass bead-making workshop dated to the period of intensive long-distance bead trade in Northeast Africa, AD 400–600.