Perceptual color space is continuous; however, we tend to divide it into only a small number of categories. It is unclear whether categorical color perception is obtained solely through the development of the visual system or whether it is affected by language acquisition. To address this issue, we recruited prelinguistic infants (5-to 7-mo-olds) to measure changes in brain activity in relation to categorical color differences by using near-infrared spectroscopy (NIRS). We presented two sets of geometric figures to infants: One set altered in color between green and blue, and the other set altered between two different shades of green. We found a significant increase in hemodynamic responses during the between-category alternations, but not during the within-category alternations. These differences in hemodynamic response based on categorical relationship were observed only in the bilateral occipitotemporal regions, and not in the occipital region. We confirmed that categorical color differences yield behavioral differences in infants. We also observed comparable hemodynamic responses to categorical color differences in adults. The present study provided the first evidence, to our knowledge, that colors of different categories are represented differently in the visual cortex of prelinguistic infants, which implies that color categories may develop independently before language acquisition.H umans can discriminate thousands of colors among continuous color space. However, we use only a handful of color terms to describe colors in our daily communication. From the analyses of data for the World Color Survey (www1.ICSI.Berkeley. EDU/wcs/), a corpus of color-naming data from 110 universal languages, many studies have revealed that particular structures of color terms used by speakers exist, and that these structures possess some common features (1, 2). Furthermore, these common features had been found even in the color perception of infants before the acquisition of the color terms (3-5). These results imply that categorical color perception may have some biological basis across cultures and languages. On the other hand, one argument for categorical color perception is that the color lexicon changes perceptual differences among colors so that colors from the same linguistic category appear much closer than colors of different categories (6, 7). A possible hypothesis is that categorical color perception has an innate perceptual foundation, and then could be modified along with the acquisition of language (8).A recent set of studies focusing on hemispheric asymmetries in categorical color perception has added another perspective to this hypothesis. Gilbert et al. (9) found that the reaction time for detecting a colored target among differently colored distractors was faster when the target and distractors belonged to different categories than when they belonged to the same category. They named this phenomenon the color-category effect, and reported that this category effect is evident only when the target was in the ...