Wound healing Activities of the bioactive compounds from Micrococcus sp. OUS9 isolated from marine water

Abstract

Marine species are increasingly important as a source of specific biological active metabolites. Marine species comprise almost half of global biodiversity. Oceans and sea are thus the biggest source of positive natural compounds that could be utilized in the pharmaceutical industry as functional constituents. In the present study was to find out the wound healing property of the bioactive compounds from Micrococcus sp . OUS9 isolated from marine source. The in vivo wound healing activity was studied using excision wound model. The KLUF 10 and KLUF13 ointment was prepared and used to determine wound healing activity in albino rats. Topical application of the ointment enhanced the contraction of wound in contrast with rat control group. KLUF13 had shown strong healing ability in wounds and had a positive influence on the various phases of wound repair.

Micrococcus spp. as a promising source for drug discovery: A review

“…As previously mentioned, KLUF-10, studied for its wound-healing activity, has since been identified as zeaxanthin (Shanthi Kumari et al., 2021 ). Antibacterial as well as promising anticancer activity of KLUF-10 is also suggested (Shanthi Kumari et al., 2020 ; Shanthi Kumari et al., 2021 ).…”

“…Excluding pigments, only six bioactive compounds isolated from Micrococcus spp. have been characterized (Su, 1948 ; Biskupiak et al., 1988 ; Bultel-Poncé et al., 1998 ; Palomo et al., 2013 ; Eltamany et al., 2014 ; Shanthi Kumari et al., 2020 ). The first bioactive compound to be fully characterized from a Micrococcus strain, micrococcin, was discovered in 1948 from a sewage isolate (Su, 1948 ).…”

“…Most recently, two wound-healing compounds, KLUF-10 (originally proposed to be 3-Hydroxy-β, ε-caroten-3′-one) and KLUF-13 (1-(1-(4-methoxphyenyl)-2-(methyl amino) ethyl) cyclohexan-1-ol) were isolated from the marine isolate Micrococcus sp. KLEF09 (originally published as strain OUS9) (Shanthi Kumari et al., 2020 ). However, KLUF-10 has since been determined by NMR analysis to in fact be zeaxanthin (Shanthi Kumari et al., 2021 ).…”

“…In fact, only eight papers describe isolated bioactive compounds (including pigments examined for bioactivity) from Micrococcus spp. (Su, 1948 ; Biskupiak et al., 1988 ; Bultel-Poncé et al., 1998 ; Mohana et al., 2013 ; Palomo et al., 2013 ; Eltamany et al., 2014 ; Shanthi Kumari et al., 2020 ; Shahin et al., 2022 ). It is notable that four of these findings pertain to marine isolates, three of which are derived from sponges.…”

The Wound-Healing Potential of Olea europaea L. Cv. Arbequina Leaves Extract: An Integrated In Vitro, In Silico, and In Vivo Investigation

“…Excisional wounds were induced in rabbits [46,47]. In summary, 0.01 mL Ketalar ® (Ketalar, Sankyo Lifetech Co., Ltd., Tokyo, Japan) was used to anesthetize the animals.…”