Wound healing activity and mechanism of action of antimicrobial and lipopolysaccharide-neutralizing peptides from enzymatic hydrolysates of rice bran proteins

Taniguchi, Masayuki; Saito, Kazuki; Aida, Ryousuke; Ochiai, Akihito; Saitoh, Eiichi; Tanaka, Takaaki

doi:10.1016/j.jbiosc.2019.02.002

Cited by 19 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…46 The effect of LL-37 on cell migration involves the functional binding to formyl peptide receptor-like 1 (FPRL1), a G protein−coupled and seventransmembrane cell receptor on endothelial cell HUVECs. 47 Likewise, as shown in the tube formation assay (Figure 4C), more tubes were formed in the PACDFe@LL-37 hydrogel group than in the control group and the PACDFe hydrogel. Quantitative analysis also confirmed that the presence of LL-37 increased the number of nodes and the total length of the vascular networks than the control group and the PACDFe hydrogel group (Figure 4D).…”

Section: Pacdfe@ll-37 Hydrogel Promoted Huvecs Function and Alleviate...supporting

confidence: 54%

“…Previous study revealed LL-37 induced keratinocyte migration through heparin-binding-EGF-mediated transactivation of epidermal growth factor receptor (EGFR) . The effect of LL-37 on cell migration involves the functional binding to formyl peptide receptor-like 1 (FPRL1), a G protein–coupled and seven-transmembrane cell receptor on endothelial cell HUVECs . Likewise, as shown in the tube formation assay (Figure C), more tubes were formed in the PACDFe@LL-37 hydrogel group than in the control group and the PACDFe hydrogel.…”

Section: Resultsmentioning

confidence: 64%

See 1 more Smart Citation

A Self-Healing Multifunctional Hydrogel System Accelerates Diabetic Wound Healing through Orchestrating Immunoinflammatory Microenvironment

Hao

Liu

Ren

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Developing an effective treatment strategy of drug delivery to improve diabetic wound healing remains a major challenge in clinical practice nowadays, due to multidrug-resistant bacterial infections, angiopathy, and oxidative damage in the wound microenvironment. Herein, an effective and convenient strategy was designed through a self-healing multiple-dynamic-bond cross-linked hydrogel with interpenetrating networks, which was formed by multiple-dynamicbond cross-linking of reversible catechol-Fe 3+ coordinate bonds, hydrogen bonding, and Schiff base bonds. The excellent autonomous healing of the hydrogel was initiated and accelerated by Schiff bonds with reversible breakage between 3,4-dihydroxybenzaldehyde containing catechol and aldehyde groups and chitosan chains, and further consolidated by the co-optation of other noncovalent interactions contributed of hydrogen bonding and Fe 3+ coordinate bonds. Intriguingly, cathelicidin LL-37 was introduced and uniformly dispersed in the dynamic interpenetrating networks of the hydrogel as a bioactive molecular to orchestrate the diabetic wound healing microenvironment. This multifunctional wound dressing can significantly promote diabetic wound healing by antibacterial activity, immunomodulation, anti-inflammation, neovascularization, and antioxidant activity. Therefore, this study provided an effective and safe strategy for guiding the diabetic wound treatment in clinical applications.

show abstract

Section: Pacdfe@ll-37 Hydrogel Promoted Huvecs Function and Alleviate...supporting

confidence: 54%

Section: Resultsmentioning

confidence: 64%

A Self-Healing Multifunctional Hydrogel System Accelerates Diabetic Wound Healing through Orchestrating Immunoinflammatory Microenvironment

Hao

Liu

Ren

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Multiple RBPs have been reported to regulate biological behaviors of vascular endothelial cells. RBP-LRR, RBP-EKL, and RBP-SSF both have been indicated to activate vascular endothelial growth factor (VEGF) to promote proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs), suggesting their potential role as wound healing agents [32]. Transcription factor RBP-J mediated Notch receptors represses proliferation of endothelial cells to maintain vascular homeostasis in adult mice.…”

Section: Discussionmentioning

confidence: 99%

SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway

Liu

Shen

Zhu

et al. 2020

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Background: Angiogenesis plays an important role in the progress of glioma. RNA-binding proteins (RBPs) and circular RNAs (circRNAs), dysregulated in various tumors, have been verified to mediate diverse biological behaviors including angiogenesis. Methods: Quantitative real-time PCR (qRT-PCR) and western blot were performed to detect the expression of SRSF10, circ-ATXN1, miR-526b-3p, and MMP2/VEGFA. The potential function of SRSF10/circ-ATXN1/miR-526b-3p axis in glioma-associated endothelial cells (GECs) angiogenesis was further studied. Results: SRSF10 and circ-ATXN1 were significantly upregulated in GECs compared with astrocyte-associated endothelial cells (AECs). Knockdown of SRSF10 or circ-ATXN1 significantly inhibited cell viability, migration and tube formation of GECs where knockdown of SRSF10 exerted its function by inhibiting the formation of circ-ATXN1. Moreover, the combined knockdown of SRSF10 and circ-ATXN1 significantly enhanced the inhibitory effects on cell viability, migration and tube formation of GECs, compared with knockdown of SRSF10 and circ-ATXN1, respectively. MiR-526b-3p was downregulated in GECs. Circ-ATXN1 functionally targeted miR-526b-3p in an RNA-induced silencing complex. Up-regulation of miR-526b-3p inhibited cell viability, migration and tube formation of GECs. Furthermore, miR-526b-3p affected the angiogenesis of GECs via negatively regulating the expression of MMP2/ VEGFA. Conclusion: SRSF10/circ-ATXN1/miR-526b-3p axis played a crucial role in regulating the angiogenesis of GECs. The above findings provided new targets for anti-angiogenic therapy in glioma.

show abstract

“…In addition, HDPs of humans, animals and plants may act as signaling molecules in innate immune defense, chemokine induction, chemotaxis, inflammation and wound healing. [ 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Induction of Chicken Host Defense Peptides within Disease-Resistant and -Susceptible Lines

Jang

Monson

Kaiser

et al. 2020

Genes

View full text Add to dashboard Cite

Host defense peptides (HDPs) are multifunctional immune molecules that respond to bacterial and viral pathogens. In the present study, bone marrow-derived cells (BMCs) and chicken embryonic fibroblasts (CEFs) were cultured from a Leghorn line (Ghs6) and Fayoumi line (M15.2), which are inbred chicken lines relatively susceptible and resistant to various diseases, respectively. The cells were treated by lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly(I:C)) and, subsequently, mRNA expression of 20 chicken HDPs was analyzed before and after the stimulation. At homeostasis, many genes differed between the chicken lines, with the Fayoumi line having significantly higher expression (p < 0.05) than the Leghorn line: AvBD1, 2, 3, 4, 6, and 7 in BMCs; CATH1, CATH3, and GNLY in CEFs; and AvDB5, 8, 9, 10, 11, 12, 13 in both BMCs and CEFs. After LPS treatment, the expression of AvBD1, 2, 3, 4, 5, 9, 12, CATH1, and CATHB1 was significantly upregulated in BMCs, but no genes changed expression in CEFs. After poly(I:C) treatment, AvBD2, 11, 12, 13, CATHB1 and LEAP2 increased in both cell types; CATH2 only increased in BMCs; and AvBD3, 6, 9, 14, CATH1, CATH3, and GNLY only increased in CEFs. In addition, AvBD7, AvBD14, CATH1, CATH2, GNLY, and LEAP2 showed line-specific expression dependent upon cell type (BMC and CEF) and stimulant (LPS and poly(I:C)). The characterization of mRNA expression patterns of chicken HDPs in the present study suggests that their functions may be associated with multiple types of disease resistance in chickens.

show abstract

Wound healing activity and mechanism of action of antimicrobial and lipopolysaccharide-neutralizing peptides from enzymatic hydrolysates of rice bran proteins

Cited by 19 publications

References 43 publications

A Self-Healing Multifunctional Hydrogel System Accelerates Diabetic Wound Healing through Orchestrating Immunoinflammatory Microenvironment

A Self-Healing Multifunctional Hydrogel System Accelerates Diabetic Wound Healing through Orchestrating Immunoinflammatory Microenvironment

SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway

Induction of Chicken Host Defense Peptides within Disease-Resistant and -Susceptible Lines

Contact Info

Product

Resources

About