Wound healing activity of Chrysophyllum cainito L. leaves: Evaluation in rats using excision wound model

Shailajan, Sunita; Gurjar, Deepti

doi:10.5530/jyp.2016.2.7

Cited by 11 publications

(10 citation statements)

References 19 publications

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“…Previously, several scientists have reported wound-healing efficacy of plant extracts. [ 30 31 32 33 ] This study investigated the role of PGE 2 during the wound-healing process that was induced by plant extract. Tissue regeneration plays important role during recovery from injury, including wound healing, and PGE 2 is candidate molecule that helps in regeneration of tissue.…”

Section: Discussionmentioning

confidence: 99%

In-vitro wound healing effect of 15-hydroxyprostaglandin dehydrogenase inhibitor from plant

Karna¹

2017

Phcog Mag

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Background:Prostaglandins (PGs) have short existence in vivo because they are rapidly metabolized by NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) to 15-ketoprostaglandins. Inhibition of 15-PGDH causes elevated level of PGE2 in cellular system. It will be valuable for the therapeutic management of diseases requiring elevated PGE2 levels, like wound healing.Objective:Ninety-eight plant samples were screened for the discovery of potent 15-PGDH inhibitor. Among them, top five plant extracts as potent 15-PGDH inhibitor were chosen to determine PGE2 release from HaCaT (Keratinocyte cell line) cell line. Finally, top 15-PGDH inhibitor was selected to evaluate in vitro wound healing effect on HaCaT scratch model.Method:The inhibitory activity for 15-PGDH inhibitors was evaluated using fluorescence spectrophotometer by measuring the formation of NADH at 468 nm following excitation at 340 nm. Cell viability assay and PGE2 release was evaluated in HaCaT cell line after treatment of 15-PGDH inhibitors. Scratches were made using sterile 200 μL on HaCaT cell and wound-healing effect was evaluated after treatment of 15-PGDH inhibitor.Results:15-PGDH inhibitors elevated PGE2 levels in concentration-dependent manner. Ethanol extract of Artocarpus heterophyllus (EEAH), the most potent 15-PGDH inhibitor (IC50 = 0.62 µg/mL) with least cytotoxicity (IC50 = 670 µg/ml), elevated both intracellular and extracellular PGE2 levels. EEAH facilitated in-vitro wound healing in a HaCaT (Keratinocyte cell line) scratch model.Conclusion:EEAH might apply to treat dermal wounds by elevating PGE2 levels via COX-1 induction and 15-PGDH inhibition.SUMMARY Biological inactivation of 15-PGDH causes elevated level of PGE2 which will be useful for the management of disease that requires elevated level of PGE2. Abbreviations used: 15-PGDH: 15-hydroxyprostaglandin dehydrogenase, COX: Cyclooxygenase, DTT: Dithiothreitol, DMEM: Dulbecco's modified Eagle's media, EEAH: Ethanol extract of Artocarpus heterophyllus, MRP4: Multidrug resistance 4, PGs: Prostaglandins, PGT: Prostaglandin transporter, SDS: Sodium dodecylsulfate

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Section: Discussionmentioning

confidence: 99%

In-vitro wound healing effect of 15-hydroxyprostaglandin dehydrogenase inhibitor from plant

Karna¹

2017

Phcog Mag

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“…e application of an ethanolic extract of C. cainito leaves on excision, soft tissue, and wounds significantly shortens the recovery time of the wound compared to control drugs (Jatyadi Taila and Betadine) [41]. An ethanolic extract of C. cainito leaves was prepared as an ointment with three different doses 2.5%, 5%, and 20%.…”

Section: Wound Healing Activity Of C Cainitomentioning

confidence: 99%

Chrysophyllum cainito: A Tropical Fruit with Multiple Health Benefits

Doan

2020

Evidence-Based Complementary and Alternative Medicine

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Chrysophyllum cainito is a tropical fruit tree with multiple benefits to human health. C. cainito possesses strong antioxidant properties either in vitro or in vivo. Extracts from the leaves, stem bark, fruits, peel, pulp, or seed of C. cainito are promising candidates in traditional medicine for curing diabetes and fighting against bacterial, fungal, and viral infections. C. cainito leaf extract alone or in a complex formula exhibits anti-inflammatory responses by reducing hypersensitivity, acts as inflammatory markers, and has antinociceptive effects. The leaf extract also increases wound healing speed and assists in regulating fat uptake. In addition, the C. cainito fruit shows anticancer activity against osteosarcoma. In conclusion, the aerial parts of C. cainito have strong beneficial biological effects on human health.

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“…The ethanolic extracts of Chrysophyllum cainito and Mimusops elengi were standardized as previously reported. [11][12] The ointment of Chrysophyllum cainito and Mimusops elengi (each containing 20% ethanolic extract) was prepared as described previously. [11][12]19 Experimental design The protocol for pharmacokinetic study was approved by Institutional Animal Ethics Committee before commencement of the study (Approval No.…”

Section: Standardization Of the Extracts And Preparation Of The Test mentioning

confidence: 99%

“…Furthermore, the traditional claim on their wound healing potential was supported using excision wound rat model. [11][12] Therefore, in the present research work, the pharmacokinetic profile of the bioactive marker gallic acid was studied post topical application of the ointment containing ethanolic extract of Chrysophyllum cainito and Mimusops elengi in male Wistar rats using a validated HPLC method. To date, several studies on pharmacokinetics of gallic acid from different biological matrices using various experimental models have been reported.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of gallic acid from the topical ointments of standardized extracts of Chrysophyllum cainito and Mimusops elengi in rats

Gurjar¹,

Shailajan²

2018

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Introduction: The wound healing activity of leaves of Chrysophyllum cainito and flowers of Mimusops elengi has been reported through our previous studies. Therefore, the aim of this study was to evaluate pharmacokinetics of gallic acid from the ethanolic extracts of these plants and to support the findings of the preclinical study. Methods: A simple HPLC method was developed for detection of gallic acid in rat plasma post topical application of the ointments containing 20% ethanolic extracts of both the plants and validated as per USFDA guidelines. Clonidine hydrochloride was used as an internal standard (IS). Chromatographic separation of gallic acid and IS was achieved on Cosmosil C 18-column using mobile phase [10 mM KH 2 PO 4 in water: acetonitrile: orthophosphoric acid (95: 5: 0.05, v/v/v)] delivered at a flow rate of 1.0 mL/min. The ultraviolet detection wavelength was set at 215 nm. Results: The calibration curve was linear over a concentration range of 0.05-5.0 μg/mL. The method was found to be accurate, precise and stable for gallic acid from spiked plasma at QC levels. The average method recoveries for gallic acid were over 85% and extraction recoveries between 78 and 87%. Conclusion: The HPLC method was applied successfully to the pharmacokinetic study of gallic acid. The detection of gallic acid in rat plasma post topical application of ethanolic extracts of both the plants proves its absorption which probably facilitates the biochemical changes and thus exhibits wound healing activity of the plant extracts.

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Wound healing activity of Chrysophyllum cainito L. leaves: Evaluation in rats using excision wound model

Cited by 11 publications

References 19 publications

In-vitro wound healing effect of 15-hydroxyprostaglandin dehydrogenase inhibitor from plant

In-vitro wound healing effect of 15-hydroxyprostaglandin dehydrogenase inhibitor from plant

Chrysophyllum cainito: A Tropical Fruit with Multiple Health Benefits

Pharmacokinetics of gallic acid from the topical ointments of standardized extracts of Chrysophyllum cainito and Mimusops elengi in rats

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