Wound Healing Activity of Crassocephalum crepidioides (Benth.) S. Moore. Leaf Hydroethanolic Extract

Abstract

Crassocephalum crepidioides (Benth.) S. Moore. has been used to treat small wounds by minority people in Lam Dong, Vietnam. However, there has been no scientific evidences about its wound healing activity. This study is aimed at evaluating the wound healing activity of Crassocephalum crepidioides hydroethanolic extract via its antioxidant and anti-inflammation activities and healing capability on a mouse excision wound model. Crassocephalum crepidioides hydroethanolic extract (CCLE) at a dose of 50 mg/kg/day reduced the wound closure time about 3.5 days, compared to vehicle treatment. The granulation tissue on day 7 after surgery from the treated group showed a 2.8-fold decrease in the density of inflammatory cells, 1.9-fold increase in the fibroblast density, and a higher number of blood vessels. Real-time PCR analysis indicated that the mRNA expression level of NF-κB1 and TNF-α mRNA in CCLE-treated wounds decreased by 4.6 and 3.3 times, respectively, while TGF-β1 and VEGF were found to increase by 3.3 and 2.4 times, respectively. Our experimental data provided proofs of Crassocephalum crepidioides leaf wound healing activity due to its antioxidant, anti-inflammation, fibroblast proliferation, wound contraction, and angiogenesis effects.

Review: Ethnomedicinal plants used for wound healing and dermatological problem in the North-Eastern Hill Region of India

“…Although there are not several scientific shreds of evidence concerning its wound healing activity, some tribals use it to treat minor wounds. The herb's wound healing effects would possibly support its capacities of antioxidant, antiinflammation, fibroblast proliferation, and angiogenesis (Can and Thao 2020). Leaf paste to cuts and wounds as hemostatic by the Chakesang Tribe of Nagaland (Nelia and Limasenla 2020).…”

“…The leaves of C. crepidioides are traditionally accustomed to treat indigestion, stomachache, and wound (Lemmens and Bunyapraphatsara 2003;Grubben and Denton 2004;Loi 2004;Adetutu et al 2011;Owokotomo et al 2012). The C. crepidioides leaf has wound healing activity due to its antioxidant, antiinflammation, fibroblast proliferation, wound contraction, and angiogenesis effects (Can and Thao 2020). Many bioactive compounds have been found in C. crepidioides leaves, including some phenoplast and flavonoid compounds; the essential oil from C. crepidioides leaves contain β-cubebene, α-farnesene, and α-caryophyllene (Can and Thao 2020).…”

Crassocephalum crepidioides (Bioactivity and Utilization)

“…The granulation tissue on day 7 after surgery of the treated group showed a 2.8-fold decrease in inflammatory cell density, a 1.9-fold increase in fibroblast density, and a higher vascular count. The wound healing activity of CC leaves is related to its activity as an antioxidant, anti-inflammatory, fibroblast proliferation, wound contraction, and the effect of angiogenesis [6].…”

“…Based on ethnomedicine research, the C. crepidioides is used to treat stomach ulcers, indigestion, wounds, ulcers, burns [2], treatment of wounds, gastric ulcers, and conditions related to the skin [5]. Local people of Lam Dong, Vietnam use C. crepidioides to treat wounds [6]. Adjatin et al [7] stated that C. crepidioides has cultivated by local communities in Benin (Africa) as a vegetable, which their consumed or to commercial purposes because of its good nutritional content.…”

Influence of hydrocortisone, dexamethasone and methylprednisolone on wound healing parameters on Nigerian indigenous dogs

“…Wounds with excessive tissue loss are allowed to heal by secondary intention, while patients receiving immunosuppressive drugs or are immunosuppressed will have suppressed inflammatory response and in the aged, hormone replacement therapy reverses age-linked conditions (Mir et al, 2018;Alemu et al, 2020). This positive response is generally enhanced with occlusive or semi-occlusive dressing (Can and Thao, 2020;Mehmet et al, 2020). Wound management is of great concern in both human and animal health and requires frequent dressing and management reviews (Mir et al, 2018;O'Brien et al, 2018).…”