Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures

Abstract

Extracts or supernatants from cultures of Lactobacilli are used for their medicinal effects, including wound healing and immune system stimulating activity. We have studied the in vivo and in vitro effects of supernatants from bacterial cultures of two strains of Lactobacillus (LS) on tissue repair and angiogenesis. Subcutaneous injection of LS into rodent ears led to proliferation of blood vessels that also exhibited strong immunostaining for Flk-1 receptor. Some inflammatory cells were scattered among the blood vessels. The continuous influx of polymorphonuclear leukocytes (PMNs) and macrophages into transcutaneous wounds in mice treated with LS resulted in prolonged inflammatory phase of wound healing and delayed wound closure, including reepithelialization. Subcutaneous injection of Matrigel impregnated with LS into the abdominal wall led to rapid and transient influx of PMNs in the vicinity of the gel. LS stimulated the proliferation of murine macrophage J774.A1 cell line and porcine lymphocytes but not that of murine fibroblast AKR-2B cells. LS also induced production of TNF-alpha by J774.A1 cells and by porcine kidney epithelial LLC-PK1 cells. LS did not appear to have an effect on collagen production. In conclusion, our study demonstrates the potential of LS to function as a stimulator of the inflammatory stage of tissue repair, TNF-alpha production, and of angiogenesis.

The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor

“…Previous studies have shown that probiotics exert diverse mechanisms, including stabilization of mucosal mast cell degranulation [29], induction of sIgA production [29], reduction in the cell apoptosis to cell proliferation ratio in the stomach [11], increase transepithelial resistance [34] and promoting angiogenesis [11] to mediate gastric ulcer healing. Here, we show that VSL#3 promoted gastric ulcer healing by enhancing the expression of growth factors, mainly VEGF by several folds.…”

Effects of concentrated supernatants recovered fromLactobacillus plantarumonEscherichia coligrowth and on the viability of a human promyelocytic cell line

“…Recently, it has been demonstrated that Lact. acidophilus supernatants promote angiogenic and chemotactic activities, which stimulate the proliferation in vitro of macrophages, lymphocytes and embryonic cells (Halper et al. 2003; Li et al.…”

“…Recently, it has been demonstrated that Lact. acidophilus supernatants promote angiogenic and chemotactic activities, which stimulate the proliferation in vitro of macrophages, lymphocytes and embryonic cells (Halper et al 2003;Li et al 2005). In addition, soluble proteins synthesized by probiotic bacteria are able to regulate intestinal epithelial homeostasis, suggesting that natural components from these microorganisms may be applied for preventing cytokinemediated gastrointestinal pathologies (Yan et al 2007).…”

Wound healing evaluation of sodium fucidate-loaded polyvinylalcohol/sodium carboxymethylcellulose-based wound dressing

“…The most important criterion is the facilitated contraction and reconstruction with less scar formation. Angiogenesis was also treated as an important criterion in wound healing, especially in the later stages of tissue repair as a process essential to restoring tissue damaged by injury and inflammation (Halper et al, 2003).…”