Wound healing and anti inflammatory effects of propolis in critical size defect of rat buccal mucosa (Histochemical and Immuno histochemical study)

Abstract

Objectives: the aim of this study is to evaluate the healing and anti-inflammatory effects of propolis in critical wound defects in rats ' buccal mucosa. Subjects& methods: 20 adult male albino rats of average weight 150-250 grams were used. Rats were divided into 2 groups Group I: consists of 10 rats, which were subdivided into 2 sub groups Sub Group I A: consists of 5 rats, the wounds were left un treated for 3 days Sub Group I B: consists of 5 rats, the wounds were left un treated for 7 days Group II: Consists of 10 rats which were subdivided into 2 sub groups Sub Group II A: consists of 5 rats, the wounds were treated with propolis for 3 days Sub Group II B: consists of 5 rats, the wounds were treated with propolis for 7 days The wounds were examined histologically, histochemically and immunohistochemically Results: Histologiacally, histochemical and immunohistochemical results showed that the healing process was faster in propolis treated rats due to anti inflammatory and anti oxidant effects of Propolis. Immunohistochemical labeling of vimentin in propolis treated groups was higher than un treated groups Conclusion: Propolis could improve wound healing by stimulating the proliferation of epithelial cells; and fibroblast proliferation, thus improving wound closure and decreasing inflammation (as prolonged inflammation and the release of inflammatory cytokines could cause tissue damage)

The Protective Effect of Propolis Nanoemulsion (NEP) Against UVB Irradiation Induced Photoaging in Human Dermal Fibroblast (HDF)

“…The cells was treated with NEP in series of concentration (0 µg/ml, 0.1 µg/ml , 0.5 µg/ml, 1 µg/ml, 10 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml) with different incubation time. HDF cells were seeded in 96 well plates, with the total cell number was 5.000 cells/well and 3Bio Journal of Biological Science, Technology and Management 2(2): [1][2][3][4][5][6][7][8][9][10] Barlian et al incubated with complete growth medium containing NEP for 24 hours, 8 hours, and 1 hour. After incubation, cells were washed with Phosphate Buffer Saline (PBS) and incubated in growth medium for 24 hours.…”

“…Propolis contains several complex natural compounds, which have potential antioxidant properties such as flavonoids and polyphenols [6]. Several studies have mentioned the role of propolis as an anti-microbe [7], anti-inflammation [8], antitumor [9,10], and antioxidant [11]. Until now, there is no study that tests propolis as an anti-photoaging property in the human skin.…”