Wound healing effect of autologous fibrin glue and polyglycolic acid sheets in a rat back skin defect model

Kouketsu, Atsumu; Shimizu, Yoshinaka; Nogami, Shinnosuke; Yamada-Fujiwara, Minami; Nagai, Hirokazu; Yamauchi, Kensuke; Matsumoto, Hitoshi; Saito, Haruka; Odashima, Kenji; Yanagisawa, Yuta; Takahashi, Tsuneo

doi:10.1016/j.transci.2021.103144

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…Compared to commercial fibrin glue, autologous fibrin glue is cheaper and has no risk of getting a blood-borne virus or having an allergic reaction. [20][21][22] Fibrin glue can modulate the activity of monocytes and macrophages, thus playing a role in the resolution of inflammation and inhibiting the TGF-β expression. Fibrin glue forms a strong clot that seals the whole length of the lesion.…”

Section: Resultsmentioning

confidence: 99%

The effect of fibrin glue on suppressing Transforming Growth Factor-β (TGF-β) expression compared to Mitomycin-C (MMC) and Triamcinolone Acetonide (TCA) as antifibrotic agents in contracted socket prevention

et al. 2022

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Background: Post-evisceration fibrosis remains the leading cause of contracted socket. The application of fibrin glue is considered an alternative adjuvant therapy in suppressing profibrotic factor (TGF-β) expression following evisceration surgery to prevent contracted socket. This study aimed to know the role of fibrin glue in suppressing the TGF-β expression following evisceration surgery. Methods: An in vivo experimental study with a randomized post-test-only control group using twenty New Zealand rabbits were conducted. The sample was divided into four groups, each consisting of five rabbits, and one healthy eye was selected randomly for evisceration. Each rabbit in the study group was given a subconjunctival injection of agents corresponding to each study group, namely: Group I (control [no injection]), Group II (0.1 ml MMC 0.4 mg/ml), Group III (0.1 ml TCA 40 mg/ml), and Group 4 (0.1 ml fibrin glue) at the inferior fornix. Following a 14-day observation period, the animals were euthanized, and tenon conjunctiva in inferior fornix samples was collected for immunohistochemistry (IHC) staining examination. The pathologist calculates the immunoreactive scores (IRS) for each specimen. The differences in TGF-β expression were statistically analyzed with a significant p<0.05. Data were analyzed using SPSS version 26 for Windows. Result: The highest TGF-β expression was found in the control group (I), and the lowest was in the mitomycin-C group (III), followed by the triamcinolone group (IV) and fibrin glue group (II). There were significant differences in TGF-β expression among the four groups (p=0.004). The difference between fibrin glue, MMC, and triamcinolone acetonide groups was insignificant (p>0.05). Conclusion: As an alternative to mitomycin-C and triamcinolone acetonide, fibrin glue can be used as adjuvant therapy to prevent constricted sockets after evisceration.

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Section: Resultsmentioning

confidence: 99%

The effect of fibrin glue on suppressing Transforming Growth Factor-β (TGF-β) expression compared to Mitomycin-C (MMC) and Triamcinolone Acetonide (TCA) as antifibrotic agents in contracted socket prevention

et al. 2022

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“…The need of this provisional matrix to support the progression of tissue repair raises fibrin to the level of a pharmacological agent with possible applications and therapeutic benefits. Although the addition of fibrin to various matrices into wound beds has been used in other studies, limited information exists about the single contribution of fibrin as a therapeutic approach for wound healing ( 28 – 30 ). In most studies, combinations of fibrin with other proteins, platelets, cells and/or growth factors were used and, therefore, these studies fall short in defining the beneficial effect of fibrin itself ( 20 , 31 – 33 ).…”

Section: Discussionmentioning

confidence: 99%

Physiological fibrin hydrogel modulates immune cells and molecules and accelerates mouse skin wound healing

et al. 2023

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IntroductionWound healing is a complex process to restore homeostasis after injury and insufficient skin wound healing is a considerable problem in medicine. Whereas many attempts of regenerative medicine have been made for wound healing with growth factors and cell therapies, simple pharmacological and immunological studies are lagging behind. We investigated how fibrin hydrogels modulate immune cells and molecules in skin wound healing in mice.MethodsPhysiological fibrin hydrogels (3.5 mg/mL fibrinogen) were generated, biophysically analyzed for stiffness and protein contents and were structurally studied by scanning electron microscopy. Physiological fibrin hydrogels were applied to full thickness skin wounds and, after 3 days, cells and molecules in wound tissues were analyzed. Leukocytes, endothelial cells, fibroblasts and keratinocytes were explored with the use of Flow Cytometry, whereas cytokines and matrix metalloproteinases were analyzed with the use of qPCR, ELISAs and zymography. Skin wound healing was analyzed microscopically at day 3, macroscopically followed daily during repair in mice and compared with commercially available fibrin sealant Tisseel.ResultsExogenous fibrin at physiological concentrations decreased neutrophil and increased non-classical Ly6Clow monocyte and resolutive macrophage (CD206+ and CX3CR1+) populations, at day 3 after injury. Fibrin hydrogel reduced the expression of pro-inflammatory cytokines and increased IL-10 levels. In line with these findings, gelatinase B/MMP-9 was decreased, whereas gelatinase A/MMP-2 levels remained unaltered. Frequencies of dermal endothelial cells, fibroblasts and keratinocytes were increased and keratinocyte migration was enhanced by fibrin hydrogel. Importantly, physiological fibrin accelerated the healing of skin wounds in contrast to the highly concentrated fibrin sealant Tisseel, which delayed wound repair and possessed a higher fiber density.ConclusionCollectively, we show that adding a tailored fibrin hydrogel scaffold to a wound bed positively influences the healing process, modulating leukocyte populations and inflammatory responses towards a faster wound repair.

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“…Physicians from a center in Nottingham applied fibrin for removal of hair cyst from the sacrococcygeal region (pilonidal cyst). After two rounds of curettage, the method was found 97% effective [9,26,[30][31][32][33][34][35][36].…”

Section: Aesthetic Medicinementioning

confidence: 99%

“…Patients who were administered fibrin glue required fewer painkillers than patients who were treated with traditional staples (H. Vossoughinia, M.A. Zarringhalam, 2020) [36].…”

Section: Other Applications Of Fibrin Gluementioning

confidence: 99%

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Fibrin glues — the current state of knowledge

Goczyńska

Lasocka

Lachert

2021

Journal of Transfusion Medicine

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Wound healing effect of autologous fibrin glue and polyglycolic acid sheets in a rat back skin defect model

Cited by 15 publications

References 18 publications

The effect of fibrin glue on suppressing Transforming Growth Factor-β (TGF-β) expression compared to Mitomycin-C (MMC) and Triamcinolone Acetonide (TCA) as antifibrotic agents in contracted socket prevention

The effect of fibrin glue on suppressing Transforming Growth Factor-β (TGF-β) expression compared to Mitomycin-C (MMC) and Triamcinolone Acetonide (TCA) as antifibrotic agents in contracted socket prevention

Physiological fibrin hydrogel modulates immune cells and molecules and accelerates mouse skin wound healing

Fibrin glues — the current state of knowledge

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