Wound Healing Effects of Chitosan Nanosheets/Honey Compounds in Male BALB/c Mice

Abstract

Background and aim: Up to now, proper wound care management has remained as an important clinical challenge. Chitosan nanosheets (CNSs) showed a great potential in tissue engineering, but our knowledge about their wound healing effectiveness is based on very limited data. Thus, the aim of this research was to evaluate the wound healing potential of CNSs and honey as a vehicle for these nanoparticles. Methods: The skin excisional wound injury model was made in adult male BALB/c mice ( n = 60) by creating two identical sized wounds (5mm) on either side of their dorsal midline. The animals were divided into five groups ( n = 12 each) as untreated control, honey, polyethylene glycol, and CNSs dissolved either in honey or polyethylene glycol. Animals were received their relative topical treatments twice per day for 14 consecutive days. Tissue sampling was carried out on days 4, 7, 10, and 14 post wounding. The histological parameters including inflammatory cells infiltration, fibroblast proliferation, re-epithelialization, granulation formation, and collagen formation were evaluated in all studied time points. Results: Compared to the control group, CNSs showed significant wound healing activities with lower inflammatory cells infiltration, higher fibroblastosis and new epithelium thickness, and greater granulation area and collagen fibers density in the ulcer bed. In addition, honey synergistically increased the wound healing activity of the studied nanoparticles. Conclusion: These results showed that CNSs have promising wound healing activity specially when dissolved with honey concurrently.

Comparative Efficacy of Chitosan with or Without Honey on Cutaneous Wound Healing in Donkeys

“…The results of the study indicated that wounds treated with chitosan and honey (Group B) showed better healing outcomes compared to wounds treated with chitosan gel alone (Group A). Similar findings have been reported in other studies, where the combination of chitosan and honey resulted in enhanced wound healing (Askari et al 2022). The enhanced wound contraction observed in Group B is in line with previous research findings (Sarhan and Azzazy 2015).…”

Chitosan-2D Nanomaterial-Based Scaffolds for Biomedical Applications

“…Chitosan nanosheets are another nanostructure which have shown excellent potential for biomedical applications. There are limited studies using CS NTs in wound healing activities with lower inflammatory cells infiltration, along with new epithelium thickness [74].…”

A clinical perspective of chitosan nanoparticles for infectious disease management

“…Further histological examination described granulation, re-epithelization, and increased collagen synthesis at the wound site [ 92 ]. Chitosan nanosheets and honey demonstrated superior wound-healing ability in male BALB/c mice with skin excisional wound injury [ 93 ].…”