Wound Healing Efficacy of Mimusops Elengi L. Flowers in Albino Wistar Rats using Excision Wound Model

Abstract

Introduction: Mimusops elengi L. (Sapotaceae), known as Bakul in Ayurveda, is an evergreen tree found throughout India. M. elengi flowers (MEF) are reported to possess immunomodulatory, hepatoprotective, skin whitening, anti-anxiety and wound healing activities which may be attributed to its phytochemical constituents including triterpenoids, alkaloids, phenolics, volatile oils, etc. Although the flowers have been traditionally used as a wound healing agent, there is a paucity of scientific data in support of its efficacy. Methods: An ethanolic extract of MEF was evaluated for its wound healing potential in male Albino Wistar rats using an excision wound model. The ethanolic extract of MEF was standardized in terms of its gallic acid content using a validated HPTLC technique. The antioxidant activity of MEF was also evaluated in vitro. The wound healing potential of the ethanolic extract of MEF was studied on the basis of wound contraction data, epithelialization period, the effect on biochemical parameters from the granulation tissue and histopathological observations. The results were compared with the traditional drug Jatyadi Taila (JT) and the modern drug Betadine (BTD). Results: Topical application of the standardized ethanolic extract of MEF on excision wounds caused a significantly faster reduction in the wound area as compared to JT and BTD. Similarly, it also showed the significant increase in the tissue hydroxyproline, hexosamine and protein biochemical parameters when compared with the untreated control. Conclusion:The findings of the present study provide data on wound healing and antioxidant potential of MEF and supports their traditional therapeutic claim.

In vivo wound healing potential and HPLC-PDA-ESI-MS profiling ofZizyphus lotusL. (Desf.) leaves methanol extract

“…The results of this study showed that ointment‐treated wounds contract faster when compared to controls. The raised wound contraction rate in MZL‐ointment group could be due to an increase in fibroblasts proliferation and their transformation into myofibroblasts (Shailajan & Gurjar, ).…”

“…Wounds treated with the methanol extract of Z. lotus leaves showed significant ( p < .05) increase in hydroxyproline, hexosamines and proteins levels of the granulation tissue in comparison with control wounds (Table ). An increase in collagen content and stabilization of its fibers might be responsible for the increase in hydroxyproline concentration after application of the MZL‐ointment (Shailajan & Gurjar, ). Hexosamines are components of glycosaminoglycans which constitute the basis of the newly formed extracellular matrix.…”

“…Re-epithelialization period was estimated as the time required for falling of scab and until normal skin replaces the wound (Shailajan & Gurjar, 2016).…”

Pharmacokinetics of gallic acid from the topical ointments of standardized extracts of Chrysophyllum cainito and Mimusops elengi in rats

“…The ethanolic extracts of Chrysophyllum cainito and Mimusops elengi were standardized as previously reported. [11][12] The ointment of Chrysophyllum cainito and Mimusops elengi (each containing 20% ethanolic extract) was prepared as described previously. [11][12]19 Experimental design The protocol for pharmacokinetic study was approved by Institutional Animal Ethics Committee before commencement of the study (Approval No.…”

“…Furthermore, the traditional claim on their wound healing potential was supported using excision wound rat model. [11][12] Therefore, in the present research work, the pharmacokinetic profile of the bioactive marker gallic acid was studied post topical application of the ointment containing ethanolic extract of Chrysophyllum cainito and Mimusops elengi in male Wistar rats using a validated HPLC method. To date, several studies on pharmacokinetics of gallic acid from different biological matrices using various experimental models have been reported.…”