Wound healing of different molecular weight of hyaluronan; in-vivo study

Fouda, Moustafa M.G.; Abdel-Mohsen, A.M.; Ebaid, Hossam; Hassan, Iftekhar; Al-Tamimi, Jameel; Abdel-Rahman, Rasha M.; Metwalli, Ali; Alhazza, Ibrahim M.; Rady, Ahmed; El‐Faham, Ayman; Jančář, J.

doi:10.1016/j.ijbiomac.2016.05.021

Cited by 60 publications

(32 citation statements)

References 42 publications

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“…We have previously reported several natural products with antioxidant and anti-inflammatory properties in vivo studies with promising results [32][33][34][35]. The present investigation has shown that rats treated with TAA have increased hepatic Nitric oxide (NO) and plasma TNF-α.…”

Section: Discussionmentioning

confidence: 67%

Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide

et al. 2018

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BackgroundThe liver disease is one of the most important traditional public health problems in Egypt. Oxidative stress is attributed to such pathological condition that further contributes to the initiation and progression of liver injury. In the present study, we have investigated if the strong antioxidant power of Nicotinamide (NA), Vitamin B2 (VB2), and Vitamin C (VC) can ameliorate TAA-induced oxidative stress-mediated liver injury in the rats.MethodsThirty-six albino rats were divided into six groups: Control group; TAA group (IP injection with TAA at a dosage of 200 mg/Kg three times a week for two months); TAA + NA group (rats administered with NA at a dosage of 200 mg/kg daily besides TAA as in the control); TAA + VB2 group (rats administered with vitamin B2 at a dosage of 30 mg/kg daily besides injection with TAA); TAA + VC group (rats administered with vitamin C at a dosage of 200 mg/kg daily along with injection of TAA). TAA + NA + VB + VC group (rats administered the with the three vitamins daily in TAA pre-injected at the respective doses described above).ResultsTreatment of rats with TAA led to a significant elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total bilirubin, cholesterol, triglycerides, low-density lipoprotein (LDL) and tumor necrosis factor-alpha (TNF-α) in the serum samples. Moreover, malondialdehyde (MDA), hydroxyproline and nitic oxide (NO) were also significantly increased in the TAA-treated rats, while reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were significantly compromised in the hepatic samples. Rats administered with NA, VB2, and VC as individually or in combination ameliorated the deleterious effects of TAA that was confirmed by histopathology. However, the combination of the three vitamins was found more effective as compared to each of the vitamins.ConclusionOur work demonstrates that NA, VB2, and VC cross-talk with each other that act as a more potent biochemical chain of antioxidant defense against TAA-induced toxicities in vivo.

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Section: Discussionmentioning

confidence: 67%

Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide

et al. 2018

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“…Moreover, BLT1 deficiency protects against systemic insulin resistance in response to obesity 28 . More studies are required to evaluate whether BLT2 participation in the regulation of glucose metabolism is part of a complementary or a parallel pathway.…”

Section: Resultsmentioning

confidence: 99%

BLT2 expression improves skin integrity and protects from alterations caused by hyperglycemia in type 2 diabetes

Leguina-Ruzzi

Valderas

2016

Dermato-Endocrinology

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Type 2 diabetes (T2D) can go undiagnosed for years, leading to a stage where chronic high blood sugar produces complications such as delayed wound healing. Reports have shown that BLT2 activation improves keratinocyte migration and wound healing, as well as protecting the epidermal barrier through the promotion of actin polymerization.The goal of this study was to elucidate the role of BLT2 expression in skin epithelial integrity in T2D.For this purpose, we used both wild type (WT) and BLT2 knockout mice in a model, in which a T2D-like phenotype was induced by keeping the animals on a high fat (HF) diet over 5 weeks. In a parallel in vitro approach, we cultured BLT2-transfected HaCaT cells at both low and high glucose concentrations for 48 h. Structure, transepithelial resistance (TEER), IL-1ß, IL-8 or CXCL2, MMP9, Filaggrin, Loricrin and Keratin 10 (K10) were evaluated ex vivo and in vitro. Additionally, wound healing (WH) was studied in vitro.The skin from T2D and BLT2 knockout mice showed a reduction in TEER and the expression of IL-1ß, and in increase in CXCL2, MMP9, Filaggrin, Loricrin and K10 expression. The structure suggested an atrophic epidermis; however, the skin was dramatically affected in the BLT2 knockout mice kept on a HF diet.HaCaT-BLT2 cells presented as an organized monolayer and showed higher TEER and wound healing compared with vector only-transfected HaCaT-Mock cells. Likewise, alterations in the expression of skin inflammatory, matrix degradation and differentiation markers under low and high glucose conditions were less severe than in HaCaT-Mock cells.Our results suggest that BLT2 improves epithelial integrity and function by regulating differentiation markers, cytokines and MMP9. Furthermore, BLT2 attenuates the damaging effects of high glucose levels, thereby accelerating wound healing.

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“…There are many ways that extensively used for the production of silica nanoparticles (SiO 2 -NPs) such as electrochemical, hydrothermal, plasma-metal hydrogen reaction, micro-emulsion, arc discharge, chemical vapor condensation, vapor phase laser pyrolysis, radiation, sonication, laser, biological, and chemical methods [12,[24][25][26][27][28][29][30][31][32][33][34][35][36]. One of these chemical methods is the sol-gel process which is extensively used in order to produce homogenous silica products in a powder form.…”

Section: Introductionmentioning

confidence: 99%

Soil Application of Nano Silica on Maize Yield and Its Insecticidal Activity Against Some Stored Insects After the Post-Harvest

El‐Naggar

Abdelsalam²,

Fouda³

et al. 2020

Nanomaterials

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103

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Maize is considered one of the most imperative cereal crops worldwide. In this work, high throughput silica nanoparticles (SiO2-NPs) were prepared via the sol–gel technique. SiO2-NPs were attained in a powder form followed by full analysis using the advanced tools (UV-vis, HR-TEM, SEM, XRD and zeta potential). To this end, SiO2-NPs were applied as both nanofertilizer and pesticide against four common pests that infect the stored maize and cause severe damage to crops. As for nanofertilizers, the response of maize hybrid to mineral NPK, “Nitrogen (N), Phosphorus (P), and Potassium (K)” (0% = untreated, 50% of recommended dose and 100%), with different combinations of SiO2-NPs; (0, 2.5, 5, 10 g/kg soil) was evaluated. Afterward, post-harvest, grains were stored and fumigated with different concentrations of SiO2-NPs (0.0031, 0.0063. 0.25, 0.5, 1.0, 2.0, 2.5, 5, 10 g/kg) in order to identify LC50 and mortality % of four common insects, namely Sitophilus oryzae, Rhizopertha dominica, Tribolium castaneum, and Orizaephilus surinamenisis. The results revealed that, using the recommended dose of 100%, mineral NPK showed the greatest mean values of plant height, chlorophyll content, yield, its components, and protein (%). By feeding the soil with SiO2-NPs up to 10 g/kg, the best growth and yield enhancement of maize crop is noticed. Mineral NPK interacted with SiO2-NPs, whereas the application of mineral NPK at the rate of 50% with 10 g/kg SiO2-NPs, increased the highest mean values of agronomic characters. Therefore, SiO2-NPs can be applied as a growth promoter, and in the meantime, as strong unconventional pesticides for crops during storage, with a very small and safe dose.

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Wound healing of different molecular weight of hyaluronan; in-vivo study

Cited by 60 publications

References 42 publications

Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide

Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide

BLT2 expression improves skin integrity and protects from alterations caused by hyperglycemia in type 2 diabetes

Soil Application of Nano Silica on Maize Yield and Its Insecticidal Activity Against Some Stored Insects After the Post-Harvest

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