Wound healing potential: evaluation of molecular profiling and amplification of Lucilia sericata angiopoietin-1 mRNA mid-part

Abstract

Objective High prevalence of chronic ulcers and the burden of disease necessitate the increasingly significant production of new recombinant proteins in the world. The angiopoietin-1 enzyme is a part of the growth factors group which is secreted by Lucilia sericata (Diptera: Calliphoridae) larvae when they meet lesions to ensure maggot therapy. It is one of the most potent proteins in wound healing. Given its essential role, the angiopoietin-1 gene of L. sericata was characterized, which provided some necessary information on its identity. Results The mid-part of the angiopoietin-1 mRNA sequence was thus characterized based on the design of different primers such as exon-exon junction, conserved regions, and specific region primers via conventional polymerase chain reaction (PCR). Its structural features were configured by in silico method. The sequence of mid-part (390 bp) of angiopoietin-1 was determined empirically, and BLAST analysis unraveled its high identity (85%) with the sequence of angiopoietin-1 mRNA of the larval housefly, Musca domestica. The homology of this enzyme also exhibited that its nucleic acid sequence was very similar to the domains of angiopoietin-1 in Lucilia cuprina. The current data are instructive and critical to evaluate the action of this enzyme in recombinant protein production in future molecular studies on wound healing.

Maggot Treatment of Necrotic Toe Developed After Traumatic Subtotal Amputation

“…24 Alipour et al showed that the angiopoietin-1 enzyme is a part of the growth factors group secreted by L sericata larvae that plays role in prenatal and neonatal vessel formation (angiogenesis) and foster wound healing. 25 High levels of γ-interferons and interleukins have been also shown in maggot secretion/excretion, which indicates the potential of future research about the effect of MDT on stimulating tissue regeneration. 26…”

“…24 Alipour et al showed that the angiopoietin-1 enzyme is a part of the growth factors group secreted by L sericata larvae that plays role in prenatal and neonatal vessel formation (angiogenesis) and foster wound healing. 25 High levels of γ-interferons and interleukins have been also shown in maggot secretion/excretion, which indicates the potential of future research about the effect of MDT on stimulating tissue regeneration. 26 In our case, MDT of the reattached subtotally amputated tissue that progressed to necrosis gave an excellent result with a normal limb both anatomically and functionally except the nail bed that showed atrophy.…”

A molecular approach to maggot debridement therapy with Lucilia sericata and its excretions/secretions in wound healing

“…Researchers suggest that this enzyme could be produced in recombinant form in the future, which would bring a different approach to wound healing. 141 T A B L E 1 The protein hits, which produced peptides with ion scores 145 The results of our studies showed that ES provided supportive effects to wound healing by regulating inflammation through NF-kB, MMP-2 and MMP-9, and by regulating the proliferation and remodelization phase over Col I, Col III, AP-1 and p53. Thus, it could be concluded that ES may have an anti-inflammatory effect, allowing the wound healing process to progress to the proliferation phase, thereby having a positive and accelerating effect on wound healing (Figure 2).…”

“…It was reported that the middle part of the mRNA sequence of Angiopoietin‐1, a growth factor known to be highly effective in wound healing and secreted by L. sericata larvae, was characterized. Researchers suggest that this enzyme could be produced in recombinant form in the future, which would bring a different approach to wound healing 141 …”

Unravelling the potential of insects for medicinal purposes – A comprehensive review

“…Lucilia sericata (Meigen) is the most used insect in maggot therapy. The excretions and secretions from maggot larvae have shown outstanding effects in wound healing, from which mainly proteins/enzymes (e.g., angiopoietin-1 enzyme [ 193 ] and serine protease [ 197 ] were determined effectively promote angiogenesis and cell proliferation. Besides, insect-derived products such as honey, bee venom, chitosan, and sericin have used in wound healing [ 233 ].…”