Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus)

Aulanni’am, A; Ora, Krismal Marchel; Ariandini, Nisa Ain; Wuragil, Dyah Kinasih; Permata, Fajar Shodiq; Riawan, Wibi; Beltran, Ma Asuncion Guiang

doi:10.14202/vetworld.2021.820-824

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Wound healing is a complex and dynamic process that involves both local and systemic cellular and biochemical responses, which is supported by many cellular events that must be closely coordinated to efficiently repair damaged tissue [ 1 , 2 ]. This process begins with a fibroblastic stage where the wound area contracts [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Wound healing by topical application of Momordica charantia L. formulations on mice

et al. 2021

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Background and Aim: Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice. Materials and Methods: Acetonic extract of M. charantia was prepared and incorporated into gel and cream formulations. Mus musculus Balb/c (n=30) with induced injury were distributed into five groups: Group I (control – day 7), Group II (control – day 14), Group III (1% gel – day 7), and Group IV (1% gel – day 14) to which 1% M. charantia gel was dermally applied daily for 7 and 14 days, respectively, Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which of M. charantia 1% cream were dermally applied daily for 7 and 14 days, respectively. Time of wound closure was determined during the experimentation; rats were euthanized with sodium pentobarbital 60 mg/kg/pc v.ip. for obtaining skin samples for histopathological analysis. Results: Groups IV and VI showed a higher percentage of wound closure on day 14, and in histopathological analysis, effect was greater in Group VI with the presence of fibroblasts and abundant collagen and elastic fibers. Conclusion: M. charantia gel and cream showed wound healing activity on induced wounded mice; the most effective treatment was M. charantia 1% cream formulation.

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Section: Introductionmentioning

confidence: 99%

Wound healing by topical application of Momordica charantia L. formulations on mice

et al. 2021

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“…To investigate the effect of resulting nanozymes on the pro-angiogenic capacity of HUVECs, cell migration assays along with tube formation assays were performed in vitro. For the cell migration assays, HUVECs were planted in 24-well plates at a cell density of 4×10 4 per well and incubated until they reached approximately 90% confluence. Subsequently, using a 200 μL sterile plastic tip to create a scratched area.…”

Section: Angiogenesis Ability Of Nanozymesmentioning

confidence: 99%

“…[1][2][3] Proper wound healing is a complicated and dynamic process associated with various cellular and metabolic activity that must take place in a coordinated and timely manner. 4,5 Failure to achieve optimal wound healing can lead to chronic wounds, increased susceptibility to infections, and impaired quality of life for affected individuals. [6][7][8] The wound healing process is a meticulously choreographed sequence of events, comprising three overlapping phases: inflammation, proliferation, and remodeling.…”

Section: Introductionmentioning

confidence: 99%

A Multifunctional Nanozyme Integrating Antioxidant, Antimicrobial and Pro-Vascularity for Skin Wound Management

Jiang,

Chen,

et al. 2024

IJN

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Background Skin wounds are a prevalent issue that can have severe health consequences if not treated correctly. Nanozymes offer a promising therapeutic approach for the treatment of skin wounds, owing to their advantages in regulating redox homeostasis to reduce oxidative damage and kill bacteria. These properties make them an effective treatment option for skin wounds. However, most of current nanozymes lack the capability to simultaneously address inflammation, oxidative stress, and bacterial infection during the wound healing process. There is still great potential for nanozymes to increase their therapeutic functional diversity and efficacy. Methods Herein, copper-doped hollow mesopores cerium oxide (Cu-HMCe) nanozymes with multifunctional of antioxidant, antimicrobial and pro-vascularity is successfully prepared. Cu-HMCe can be efficiently prepared through a simple and rapid solution method and displays sound physiological stability. The biocompatibility, pro-angiogenic, antimicrobial, and antioxidant properties of Cu-HMCe were assessed. Moreover, a full-thickness skin defect infection model was utilized to investigate the wound healing capacity, as well as anti-inflammatory and pro-angiogenic properties of nanozymes in vivo. Results Both in vitro and in vivo experiments have substantiated Cu-HMCe’s remarkable biocompatibility. Moreover, Cu-HMCe possesses potent antioxidant enzyme-like catalytic activity, effectively clearing DPPH radicals (with a scavenging rate of 80%), hydroxyl radicals, and reactive oxygen species. Additionally, Cu-HMCe exhibits excellent antimicrobial and pro-angiogenic properties, with over 70% inhibition of both E. coli and S. aureus . These properties collectively promote wound healing, and the wound treated with Cu-HMCe achieved a closure rate of over 90% on the 14th day. Conclusion The results indicate that multifunctional Cu-HMCe with antioxidant, antimicrobial, and pro-angiogenic properties was successfully prepared and exhibited remarkable efficacy in promoting wound healing. This nanozymes providing a promising strategy for skin repair.

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“…Molina-Botero et al studied its active substances, including flavonoids, saponins, tannins, alkaloids, polyphenols, hydroxyl acid, and coumarin [23]. Aulanni'am et al use G. sepium leaves can heal excision wounds with their anti-inflammatory effect because it contains bioactive compounds to enhance the wound healing process [24]. According to research by Carandang et al wound treated with 7.5% gel G. sepium on excision wound is safe, effective, and stable [25].…”

Section: Since 2002mentioning

confidence: 99%

Epidermal Stem Cell in Wound Healing of Gliricidia sepium Leaves from Indonesia and the Philippines in Rats (Rattus norvegicus)

Aulanni’am

Raissa

Riawan

et al. 2022

Open Access Maced J Med Sci

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AIM: This study intended to investigate the regenerate wound, due to the ointment therapy containing Gliricidia sepium leaves that has potential-induced epidermal stem cells producing. It determined its effect on the expression of transforming growth factor-β1 (TGF-β1), Smad-3, β-catenin, LGR-6. MATERIALS AND METHODS: About 16 Wistar male rats aged approximately 2 months (150–200g) were used and were divided into four treatment groups (T1, positive control; T2, negative control; T3, wounds treated with G. sepium from Indonesia; and T4, wounds treated with G. sepium from the Philippines). The treatment of ointment was applied to the wound for 3 days. The expression of TGF-β1, Smad-3, β-catenin, and LGR-6 was observed by immunohistochemistry staining. RESULTS: G. sepium leaves significantly (p < 0.05) upregulated the expression of TGF-β1, Smad-3, β-catenin, and LGR-6 in the group treated with Indonesian G. sepium leaves were higher than that in the group treated with G. sepium leaves from the Philippines. CONCLUSIONS: Both leaves Varian contain flavonoids, saponins, and tannins, which act as producing epidermal stem cell agents to enhance the wound healing process. It can be concluded that both Gl. sepium Varian Indonesia and the Philippines have a potential effect on wound healing.

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Wound healing properties of Gliricidia sepium leaves from Indonesia and the Philippines in rats (Rattus norvegicus)

Cited by 9 publications

References 10 publications

Wound healing by topical application of Momordica charantia L. formulations on mice

Wound healing by topical application of Momordica charantia L. formulations on mice

A Multifunctional Nanozyme Integrating Antioxidant, Antimicrobial and Pro-Vascularity for Skin Wound Management

Epidermal Stem Cell in Wound Healing of Gliricidia sepium Leaves from Indonesia and the Philippines in Rats (Rattus norvegicus)

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