Wound‐healing properties of trehalose‐stabilized freeze‐dried outdated platelets

Sum, Ruth; Hager, Sarah; Pietramaggiori, Giorgio; Orgill, Dennis P.; Dee, Josh; Rudolph, Alan S.; Orser, Cindy S.; Fitzpatrick, G. Michael; Ho, David

doi:10.1111/j.1537-2995.2007.01170.x

Cited by 38 publications

(25 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To date, the collection of whole blood and the concentration and isolation of platelets to make PRP has been proven effective in vitro for stimulating cellular activity in a number of formats, both in liquid 13,[33][34][35]37,[39][40][41] and in lyophilized PRGF form. [16][17][18][19] To the best of the authors' knowledge, this article serves as the first instance of a powdered PRGF being incorporated into an electrospun tissue engineering scaffold to serve as a controlled release vehicle for such a concentrated growth factor and cytokine milieu. While electrospun scaffolds have been used as growth factor delivery systems in the past, [42][43][44][45][46][47] they have typically been limited to the incorporation of only a small number of growth factors due in part to the cost associated with purchasing the recombinant or isolated proteins.…”

Section: Discussionmentioning

confidence: 99%

“…14 The use of freeze-dried PRP in a dermal wound has been shown to significantly increase cellular proliferation (up to 21 days), tissue regeneration, and angiogenesis in a mouse dermal wound. [16][17][18][19] Collectively, these studies demonstrate the importance of keeping PRPderived growth factors and cytokines in the wound site and slowly releasing them as the wound site becomes infiltrated with reparative cells.…”

Section: Introductionmentioning

confidence: 99%

“…Other techniques have been evaluated for further sustaining the release of growth factors from PRP and include the use of gelatin gel microspheres, 15 lyophilized PRP, [16][17][18][19] and alginate beads. 14 The injection of PRP gelatin gel microspheres in a mouse ischemic hind limb model demonstrated sustained release of the PRP potent angiogenic components as illustrated by an increase in perfusion, capillary density, and mature blood vessel density.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) therapy has seen a recent spike in clinical interest due to the potential that the highly concentrated platelet solutions hold for stimulating tissue repair and regeneration. The aim of this study was to incorporate PRP into a number of electrospun materials to determine how growth factors are eluted from the structures, and what effect the presence of these factors has on enhancing electrospun scaffold bioactivity. PRP underwent a freeze-thaw-freeze process to lyse platelets, followed by lyophilization to create a powdered preparation rich in growth factors (PRGF), which was subsequently added to the electrospinning process. Release of protein from scaffolds over time was quantified, along with the quantification of human macrophage and adipose-derived stem cell (ADSC) chemotaxis and proliferation. Protein assays demonstrated a sustained release of protein from PRGF-containing scaffolds at up to 35 days in culture. Scaffold bioactivity was enhanced as ADSCs demonstrated increased proliferation in the presence of PRGF, whereas macrophages demonstrated increased chemotaxis to PRGF. In conclusion, the work performed in this study demonstrated that the incorporation of PRGF into electrospun structures has a significant positive influence on the bioactivity of the scaffolds, and may prove beneficial in a number of tissue engineering applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Freeze-dried platelets have been investigated for wound repair with encouraging results. [27][28][29] The primary objective of this study was to compare the effect of PP to PRP and BMA on gene expression of FDS and SL explants. In addition, we sought to evaluate the temporal release of the growth factors TGF-b1 and PGDF from PP, PRP, and BMA when used as media in explant culture to confirm that there is a correlation between platelet and growth factor concentration.…”

mentioning

confidence: 99%

Temporal growth factor release from platelet‐rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression

McCarrel

Fortier

2009

Journal Orthopaedic Research

324

254

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) has generated substantial interest for tendon and ligament regeneration because of the high concentrations of growth factors in platelet a-granules. This study compared the temporal release of growth factors from bone marrow aspirate (BMA), PRP, and lyophilized platelet product (PP), and measured their effects on tendon and ligament gene expression. Blood and BMA were collected and processed to yield PRP and plasma. Flexor digitorum superficialis tendon (FDS) and suspensory ligament (SL) explants were cultured in 10% plasma in DMEM (control), BMA, PRP, or PP. TGF-b1 and PDGF-BB concentrations were determined at 0, 24, and 96 h of culture using ELISA. Quantitative RT-PCR for collagen types I and III (COL1A1, COL3A1), cartilage oligomeric matrix protein (COMP), decorin, and matrix metalloproteinases-3 and 13 (MMP-3, MMP-13) was performed. TGF-b1 and PDGF-BB concentrations were highest in PRP and PP. Growth factor quantity was unchanged in BMA, increased in PRP, and decreased in PP over 4 days. TGF-b1 and platelet concentrations were positively correlated. Lyophilized PP and PRP resulted in increased COL1A1:COL3A1 ratio, increased COMP, and decreased MMP-13 expression. BMA resulted in decreased COMP and increased MMP-3 and MMP-13 gene expression. Platelet concentration was positively correlated with COL1A1, ratio of COL1A1:COL3A1, and COMP, and negatively correlated with COL3A1, MMP-13, and MMP-3. White blood cell concentration was positively correlated with COL3A1, MMP3, and MMP13, and negatively correlated with a ratio of COL1A1:COL3A1, COMP, and decorin. These findings support further in vivo investigation of PRP and PP for treatment of tendonitis and desmitis. ß

show abstract

“…However, as demonstrated by flow cytometric investigations, these platelets showed elevated levels of various platelet activation markers, which could be interpreted as a form of platelet damage. Recent activities focus on the use of freeze-dried platelets in topical wound healing [59], as in such preparations the platelets do not necessarily need to be viable.…”

Section: Membrane Modification Strategiesmentioning

confidence: 99%

Membrane Stability during Biopreservation of Blood Cells

Stoll¹,

Wolkers²

2011

Transfus Med Hemother

View full text Add to dashboard Cite

Storage methods, which can be taken into consideration for red blood cells and platelets, include liquid storage, cryopreservation and freeze-drying. Red blood cells can be hypothermically stored at refrigerated temperatures, whereas platelets are chilling sensitive and therefore cannot be stored at temperatures below 20°C. Here we give an overview of available cryopreservation and freeze-drying procedures for blood cells and discuss the effects of these procedures on cells, particularly on cellular membranes. Cryopreservation and freeze-drying may result in chemical and structural modifications of cellular membranes. Membranes undergo phase and permeability changes during freezing and drying. Cryo- and lyoprotective agents prevent membrane damage by different mechanisms. Cryoprotective agents are preferentially excluded from membrane surfaces. They decrease the activation energy for water transport during freezing and control the rate of cellular dehydration. Lyoprotectants are thought to stabilize membranes during drying by forming direct hydrogen bonding interactions with phospholipid head groups. In addition, lyoprotectants can form a glassy state at room temperature. Recently liposomes have been investigated to stabilize blood cells during freezing and freeze-drying. Liposomes modify the composition of cellular membranes by lipid and cholesterol transfer, which can stabilize or destabilize the low temperature response of cells.

show abstract

Wound‐healing properties of trehalose‐stabilized freeze‐dried outdated platelets

Abstract: FDPOs enhanced wound healing in db/db mice as well as FDPIs and RT-PLTs. Wound closure was obtained 6 days earlier than untreated wounds and histologic examination revealed reduced granulation and increased cellular angiogenesis.

Cited by 38 publications

References 30 publications

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Temporal growth factor release from platelet‐rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression

Membrane Stability during Biopreservation of Blood Cells

Contact Info

Product

Resources

About