Wound healing property of a gel prepared by the combination ofPseudomonas aeruginosaalginate andAlhagi maurorumaqueous extract in rats

Abstract

Although alginate has been known to be a good wound dressing, it does not have antimicrobial properties, has low availability, and is expensive. To overcome these problems, the present study was conducted, where the extraction of this material from an available small factory Pseudomonas aeruginosa and the improvement of its wound healing property by its combination with herb extract, Alhagi maurorum, done. Nineteen P. aeruginosa strains were isolated and identified from burned skin, and the one isolated strain with the highest ability of alginate production was selected. A. maurorum aqueous extract was prepared, and the toxicity of each material was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide assay. A mixture of nontoxic doses of each substance was then prepared. Thirty-two Wistar rats were divided into four groups (n = 8). The control group and the rest three groups, which were treated by alginate, A. maurorum extract, and alginate-A. maurorum extract. Throughout the 21 days of treatment, the open wound sites were checked. Finally, the rats were sacrificed and the effect of each substance on their skin tissue was evaluated. The results showed that the high alginate production without any toxic effect was obtained from the P. aeruginosa strain K1. A. maurorum aqueous extract had dose-dependent toxicity. The aqueous solution of alginate-A. maurorum extract complex group showed the best wound healing activity in both macroscopic and microscopic examinations. Recent research has introduced a new type of wound dressing with high wound healing properties. This could decrease the time for re-epithelialization and increase wound contraction percentage. K E Y W O R D Salginate, Alhagi maurorum, Pseudomonas aeruginosa, wound healing

UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Alhagi maurorum against Oral Ulcer in Rats

“…The best healing effect was found in the EFAM-treated group at the end of the 14-days treatment period. Pourali and Yahyaei [ 55 ] studied the role of A. maurorum in enhancing wound healing through tissue remodeling and collagen deposition, which is in agreement with the results of the present study. Ulcer healing is a complex programmed process that requires epithelial cell migration and proliferation for continued re-epithelialization [ 56 ].…”

Biomedical Applications of Bacterial Exopolysaccharides: A Review

“…Tissue engineering and bone regeneration, drug delivery, cancer diagnosis (CD44 interaction) [25] Cyanobacteria Wound healing [26] Composite scaffold for skin regeneration [27] Alginate Wound dressing containing Alhagi maurorum extract [28] Wound dressing, scaffold for drug delivery [29] Hydrogels for wound dressing [23] Pseudomonas aeruginosa Alginate-chitosan hydrogels for human cell encapsulation [30] Azotobacter vinelandii Alginate-based hydrogel for inducing retinal pigment epithelium regeneration [31] Nostoc sp. Aerogel for drug delivery and tissue engineering [25] Pseudomonas, Azotobacter…”

Physicochemical Characterization and Immunomodulatory Activity of Polyelectrolyte Multilayer Coatings Incorporating an Exopolysaccharide from Bifidobacterium longum

“…Bacterial exopolysaccharides in general display good biocompatibility, biodegradability, and nontoxicity, making them suitable candidates for biomedical engineering. The most widely used bacterial EPS in biomedical applications include cellulose, which is used due to its excellent mechanical properties and high water retention capabilities, alginate due to the facile cross-linking potential, and xanthan gum due to its excellent water solubility, biocompatibility, immune inertness, and positive viscoelastic properties . Such bacterial EPS have been used in a number of biomedical engineering applications, including for bone regeneration and tissue engineering as well as in wound healing and osteoarthritis …”