Wound-healing response and refractive regression after conductive keratoplasty

Esquenazi, Salomon; He, Jiucheng; Kim, Dooho B.; Bazán, Nicolás G.; Bui, Viet Trang; Bazán, Haydee E. P.

doi:10.1016/j.jcrs.2005.12.077

Cited by 22 publications

(23 citation statements)

References 21 publications

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“…This fact confirms from a mechanical standpoint that the woundhealing is very likely to play the major role in the commonly observed decrease in the initial degree of the refractive correction (Esquenazi et al 2006). …”

Section: Introductionsupporting

confidence: 75%

“…A cinching effect is thus obtained that increases the curvature of the central cornea, yielding the desired result. Although the procedure is far less invasive and risky than ablative ones, the post-operative follow-up shows that the initial degree of refractive correction tends to decrease 20% in 24 months' time (McDonald 2005;Esquenazi et al 2006) so that the predictability of the outcome cannot be considered completely satisfactory.…”

Section: Introductionmentioning

confidence: 99%

“…However, the viscoelastic behaviour gives reason from a mechanical standpoint for the immediate corneal steepening in rabbits corneae observed after CK by Esquenazi et al (2006).…”

Section: Introductionmentioning

confidence: 99%

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The role of viscoelasticity and stress gradients on the outcome of conductive keratoplasty

Fraldi

Cutolo

Esposito

et al. 2010

Biomech Model Mechanobiol

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A mechanical analysis of the conductive keratoplasty on hyperopic eyes has been carried out, and the attention has been focused on incorporating the actual viscoelastic properties of the human corneal tissue and on the stress gradients induced by the intervention. By avoiding unnecessary complications which may obscure the essential behaviour of the model, the results are in very good agreement with the clinical and experimental findings and suggest that the major role in the commonly observed decrease of the initial degree of the refractive correction might be played by the stress gradients at the intervention spots, which are likely to influence the wound-healing. The study aims to contribute some firm mechanical roots to the predictability of the outcome of an increasingly popular technique that, notwithstanding several advantages with respect to ablative interventions, at present cannot be considered completely satisfactory.

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Section: Introductionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

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The role of viscoelasticity and stress gradients on the outcome of conductive keratoplasty

Fraldi

Cutolo

Esposito

et al. 2010

Biomech Model Mechanobiol

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“…An important feature of CEC is their ability to synthesize and deposit collagens into their ECM. Collagen synthesis was investigated previously in corneal endothelium in vivo and in vitro in several species at different developmental stages [Ben-Zvi et al, 1986;Kay et al, 1993;Levy et al, 1995;Funderburgh et al, 2003;Esquenazi et al, 2006]. It could be shown that collagen IV is the main collagen produced by normal CEC and is also the main component of Descemet's membrane, while collagen III can be detected in the ECM of normal bovine CEC, but is barely detectable in the ECM of normal rabbit CEC [Kay et al, 1993].…”

Section: Clonal Cell Lines Of Human Corneal Endothelium Cells Tissuesmentioning

confidence: 99%

“…Only few groups reported on collagen III in the human corneal endothelium; for example, BenZvi et al [1986] state that collagen III was detected in the human cornea during embryonic development in Descemet's and Bowman's membrane, but could not be detected in the central cornea anymore after the 27th week of gestation. On the other hand, other groups reported the finding of collagen III during corneal wound healing [Funderburgh et al, 2003;Esquenazi et al, 2006] or in pathologic matrix deposits in the diseased corneal endothelium [Levy et al, 1995]. It is known from histological examinations of retrocorneal fibrous membranes that HCEC can transdifferentiate towards a fibroblastoid phenotype.…”

Section: Clonal Cell Lines Of Human Corneal Endothelium Cells Tissuesmentioning

confidence: 99%

Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics

Valtink

Gruschwitz

Funk

et al. 2008

Cells Tissues Organs

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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastructure was monitored by scanning and transmission electron microscopy. Two clonal cell lines, HCEC-B4G12 and HCEC-H9C1, could be isolated and expanded, which differed morphologically: B4G12 cells were polygonal, strongly adherent and formed a strict monolayer, H9C1 cells were less adherent and formed floating spheres. The generation time of B4G12 cells was 62.26 ± 14.5 h and that of H9C1 cells 44.05 ± 5.05 h. Scanning electron microscopy revealed that B4G12 cells had a smooth cell surface, while H9C1 cells had numerous thin filopodia. Both cell lines expressed ZO-1 and occludin adequately, and little but well detectable amounts of connexin-43. Expression of HCEC-specific 9.3.E-antigen was found commensurately in both cell lines, while expression of Na,K-ATPase α1 was higher in H9C1 cells than in B4G12 cells. B4G12 cells expressed collagen IV abundantly and almost no collagen III, while H9C1 cells expressed both collagens at reasonable amounts. It is concluded that the clonal cell line B4G12 represents an ideal model of differentiated HCEC, while H9C1 may reflect features of developing or transitional HCEC.

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Outcomes and complications of surface ablation

Onguchi¹,

Azar²

2009

Corneal Surgery

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Wound-healing response and refractive regression after conductive keratoplasty

Abstract: The histological changes that occur after CK may be responsible for the well-established regression of its refractive effect. A better understanding of the wound-healing response after CK is necessary to improve the long-term stability of the procedure.

Cited by 22 publications

References 21 publications

The role of viscoelasticity and stress gradients on the outcome of conductive keratoplasty

The role of viscoelasticity and stress gradients on the outcome of conductive keratoplasty

Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics

Outcomes and complications of surface ablation

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