Wrapping Bacteria by Graphene Nanosheets for Isolation from Environment, Reactivation by Sonication, and Inactivation by Near-Infrared Irradiation

Akhavan, Omid; Ghaderi, Elham; Esfandiar, Ali

doi:10.1021/jp200686k

Cited by 614 publications

(437 citation statements)

References 98 publications

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“…37 The direct-contact interaction of extremely sharp edges of graphene nanowalls with the membrane of cells is also known as one of the mechanisms effectively involved in the cytotoxicity of graphene sheets. 38 Trapping microorganisms within aggregate-reduced graphene sheets was also suggested as another mechanism for describing the cytotoxicity of graphene sheets, especially in a suspension. 38 The size-dependent cyto-and genotoxic effects of graphene sheets and nanoplates have been evaluated in human mesenchymal stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…38 Trapping microorganisms within aggregate-reduced graphene sheets was also suggested as another mechanism for describing the cytotoxicity of graphene sheets, especially in a suspension. 38 The size-dependent cyto-and genotoxic effects of graphene sheets and nanoplates have been evaluated in human mesenchymal stem cells. 39 Taking this literature into account, we investigated the antibacterial activity of graphene materials in Pseudomonas aeruginosa, which is a common Gram-negative bacterium that can cause disease in humans and animals.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Gurunathan¹,

Han²,

Dayem³

et al. 2012

IJN

739

511

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Background: Graphene holds great promise for potential use in next-generation electronic and photonic devices due to its unique high carrier mobility, good optical transparency, large surface area, and biocompatibility. The aim of this study was to investigate the antibacterial effects of graphene oxide (GO) and reduced graphene oxide (rGO) in Pseudomonas aeruginosa. In this work, we used a novel reducing agent, betamercaptoethanol (BME), for synthesis of graphene to avoid the use of toxic materials. To uncover the impacts of GO and rGO on human health, the antibacterial activity of two types of graphene-based material toward a bacterial model P. aeruginosa was studied and compared. Methods: The synthesized GO and rGO was characterized by ultraviolet-visible absorption spectroscopy, particle-size analyzer, X-ray diffraction, scanning electron microscopy and Raman spectroscopy. Further, to explain the antimicrobial activity of graphene oxide and reduced graphene oxide, we employed various assays, such as cell growth, cell viability, reactive oxygen species generation, and DNA fragmentation. Results: Ultraviolet-visible spectra of the samples confirmed the transition of GO into graphene. Dynamic light-scattering analyses showed the average size among the two types of graphene materials. X-ray diffraction data validated the structure of graphene sheets, and high-resolution scanning electron microscopy was employed to investigate the morphologies of prepared graphene. Raman spectroscopy data indicated the removal of oxygen-containing functional groups from the surface of GO and the formation of graphene. The exposure of cells to GO and rGO induced the production of superoxide radical anion and loss of cell viability. Results suggest that the antibacterial activities are contributed to by loss of cell viability, induced oxidative stress, and DNA fragmentation. Conclusion:The antibacterial activities of GO and rGO against P. aeruginosa were compared. The loss of P. aeruginosa viability increased in a dose-and time-dependent manner. Exposure to GO and rGO induced significant production of superoxide radical anion compared to control. GO and rGO showed dose-dependent antibacterial activity against P. aeruginosa cells through the generation of reactive oxygen species, leading to cell death, which was further confirmed through resulting nuclear fragmentation. The data presented here are novel in that they prove that GO and rGO are effective bactericidal agents against P. aeruginosa, which would be used as a future antibacterial agent.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Gurunathan¹,

Han²,

Dayem³

et al. 2012

IJN

739

511

View full text Add to dashboard Cite

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“…The antimicrobial properties of graphenebased materials have been thoroughly evaluated by many researchers [22][23][24][25]. The antibacterial activity of graphene oxide and graphene is attributed to the significant membrane stress induced by the sharp edges of their nanosheets, which may result in physical damage to cell membranes, leading to the loss of bacterial membrane integrity and the leakage of RNA [25,26].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Electrochemical synthesis of nanosized hydroxyapatite/graphene composite powder

Mišković‐Stanković¹,

Eraković²,

Jankovic³

et al. 2015

Carbon letters

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Electrochemical synthesis was employed to prepare a novel hydroxyapatite/graphene (HAP/ Gr) composite powder suitable for medical applications as a hard tissue implant (scaffold). The synthesis was performed in a homogeneous dispersion containing Na 2 H 2 EDTA·2H 2 O, NaH 2 PO 4 and CaCl 2 with a Ca/EDTA/PO 4 3− concentration ratio of 0.25/0.25/0.15M, along with 0.01 wt% added graphene nanosheets, at a current density of 137 mA cm −2 and pH value of 9.0. The field emission scanning electron microscopy and transmission electron microscopy observations of the composite HAP/Gr powder indicated that nanosized hydroxyapatite particles were uniformly placed in the graphene overlay. Raman spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction confirmed graphene incorporation in the HAP/Gr powder. The electrochemically prepared HAP/Gr composite powder exhibited slight antibacterial effect against the growth of the bacterial strain Staphylococcus aureus.

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“…In addition, controversial results were reported about RGO antimicrobial activity toward Gram negative and Gram positive bacteria (5,9).…”

Section: Introductionmentioning

confidence: 99%

Preparation and Biological Activity of New Collagen Composites Part II: Collagen/Reduced Graphene Oxide Composites

et al. 2017

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With the idea of exploring the biological activity of some newly synthetized chemical compounds and their combinations for development of novel antimicrobial collagen biomaterials, a serial investigation was initiated, starting with the preparation and biological activity study of Collagen/ZnTiO3 nano-composites. This serial investigation continued with the preparation and biological activity study of new collagen-based composites in which self-prepared reduced graphene oxide (RGO) sheets were included as an antimicrobial agent. The new porous collagen/RGO composites demonstrated specific antimicrobial activity to different types microbial species; well pronounced activity against Gram-positive microorganisms (Listeria innocua and Bacillus cereus, both bacteria with typical chains forming, large size cells, and Candida lusitaniae, fungus with specific micelle organization) and lack of activity against Gram-negative bacteria (Pseudomonas putida, Salmonella enterica, Pseudomonas aeruginosa, and Escherichia coli; all bacteria with small size cells) combined with lack of cytotoxicity to eukaryotic cells. For the first time, well-pronounced antifungal activity of collagen/RGO composites, depending on the RGO concentration was observed. Sterile zone of 17 mm was measured for C. lusitaniae on collagen/RGO composite, 2:1 wt/wt. The possible mechanism of the biological activity of the new collagen/RGO composites was correlated with their characteristics and the specific cell morphology and size of the test microorganisms. The results of this investigation demonstrated that with their specific and adjustable bioactivity, the new collagen/RGO composites are promising antimicrobial biomaterial for variety of biomedical applications, including tissue engineering.

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Wrapping Bacteria by Graphene Nanosheets for Isolation from Environment, Reactivation by Sonication, and Inactivation by Near-Infrared Irradiation

Cited by 614 publications

References 98 publications

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Electrochemical synthesis of nanosized hydroxyapatite/graphene composite powder

Preparation and Biological Activity of New Collagen Composites Part II: Collagen/Reduced Graphene Oxide Composites

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