Wrapping With Basic Fibroblast Growth Factor‐Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia

Mukai, Michiaki; Uchida, Kentaro; Hirosawa, Naoya; Murakami, Kenichi; Kuniyoshi, Kazuki; Inoue, Gen; Miyagi, Masayuki; Sekiguchi, Hiroyuki; Inage, Kazuhide; Orita, Sumihisa; Suzuki, Takane; Matsuura, Yusuke; Takaso, Masashi; Ohtori, Seiji

doi:10.1002/jor.24349

Cited by 11 publications

(17 citation statements)

References 30 publications

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“…Here, frozen vein contained a higher amount of bFGF than sciatic nerve. We previously reported that bFGF stimulated rat sciatic nerve in vitro and that bFGF-absorbed collagen sheet wrapping promoted Hmox1 expression and improved mechanical allodynia [23]. Our present observation suggests that ECM-derived bFGF in vein may induce Hmox1 expression.…”

Section: Discussionsupporting

confidence: 66%

“…Previous studies proposed the mechanism that autologous vein wrapping exerted its therapeutic effect through vein-derived trophic factors [9,10,23]. We previously reported that Hmox1 following fresh VW and recombinant bFGF stimulate Hmox1 expression in sciatic nerve cells in vitro [9].…”

Section: Discussionmentioning

confidence: 97%

“…von Frey Test Rats (n = 5 per group) were subjected to the von Frey test according to our previous report [9][10][11]23]on postoperative days (PODs) 1, 3, 5, 7, and 14. Following randomization and 1-h acclimation to the test cage, the von Frey test was conducted by applying a von Frey lament (Mono-lament Kit; Smith & Nephew, Germantown, WI) to the hind paw perpendicular to the plantar surface.…”

Section: Cell Viability Of Frozen Veinmentioning

confidence: 99%

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Frozen Vein Wrapping for Chronic Nerve Constriction Injury Reduces Sciatic Nerve Allodynia in a Rat Model

Mukai¹,

Uchida²,

Hirosawa³

et al. 2021

Preprint

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BackgroundAutologous vein wrapping (VW) is used in the treatment of recurrent chronic constriction neuropathy and traumatic peripheral nerve injury. However, use of autologous veins is limited by the inability to obtain longer veins of sufficient length for larger sites. Frozen allograft tissue has several advantages, including its availability for large grafts, avoidance of donor-site morbidity, and shorter operation time. Here, we investigated the effect of frozen vein wrapping (FVW) in Wistar rats as a model of sciatic nerve injury. MethodsThe rats were grouped by treatment as (i) untreated after chronic constriction injury surgery (CCI; control group), (ii) treated with vein wrapping using freshly isolated vein (VW), and (iii) treated with vein wrapping using frozen vein (FVW). Mechanical allodynia was assessed with von Frey filaments on postoperative days (PODs) 1, 3, 5, 7, and 14. ResultsThe response of heme oxygenase-1 gene, Hmox-1, expression to VW and FVW was assessed by RT-PCR. Both VW and FVW significantly increased withdrawal threshold levels compared to the untreated control group on POD 1, 3, and 5. Both VW and FVW also showed increased HO-1 expression compared to the CCI group. ConclusionsOur results suggest that FVW may be a suitable therapeutic optionas a source of large grafts.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 97%

Section: Cell Viability Of Frozen Veinmentioning

confidence: 99%

See 1 more Smart Citation

Frozen Vein Wrapping for Chronic Nerve Constriction Injury Reduces Sciatic Nerve Allodynia in a Rat Model

Mukai¹,

Uchida²,

Hirosawa³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rats ( n = 5 per group) were subjected to the von Frey test according to our previous report [ 9 – 11 , 24 ] on postoperative days (PODs) 1, 3, 5, 7, and 14. Following randomization and 1-h acclimation to the test cage, the von Frey test was conducted by applying six von Frey filaments (3.922 mN, 5.882 mN, 9.804 mN, 13.725 mN, 19.608 mN, 39.216 mN) (Mono-filament Kit; Smith & Nephew, Germantown, WI) to the hind paw perpendicular to the plantar surface.…”

Section: Methodsmentioning

confidence: 99%

Frozen vein wrapping for chronic nerve constriction injury reduces sciatic nerve allodynia in a rat model

et al. 2022

Self Cite

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Background Autologous vein wrapping (VW) is used in the treatment of recurrent chronic constriction neuropathy and traumatic peripheral nerve injury. However, use of autologous veins is limited by the inability to obtain longer veins of sufficient length for larger sites. Frozen allograft tissue has several advantages, including its availability for large grafts, avoidance of donor-site morbidity, and shorter operation time. Here, we investigated the effect of frozen vein wrapping (FVW) in Wistar rats as a model of sciatic nerve injury. Results The rats were grouped by treatment as (i) untreated after chronic constriction injury surgery (CCI; control group), (ii) treated with vein wrapping using freshly isolated vein (VW), and (iii) treated with vein wrapping using frozen vein (FVW). Mechanical allodynia was assessed with von Frey filaments on postoperative days (PODs) 1, 3, 5, 7, and 14. Gene expression of HO-1 was evaluated by quantitative polymerase chain reaction (qPCR). The response of heme oxygenase-1 gene, Hmox-1, expression to VW and FVW was assessed by RT-PCR. Both VW and FVW significantly increased withdrawal threshold levels compared to the untreated control group on POD 1, 3, and 5. Both VW and FVW also showed increased HO-1 expression compared to the CCI group. Conclusions FVW increased the withdrawal threshold similar to VW in a rat CCI model for short periods. Frozen vein wrapping using vein allograft without donor site morbidity may be an alternative therapeutic option.

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“…Dysesthesia involves a disagreeable sensation such as a painful burning, prickling or aching feeling, and allodynia is defined as an unpleasant sensation that occurs with light touch. Sciatic nerve injury (SNI) is more closely related to allodynia than to dysesthesia ( Mukai et al, 2019 ). Patients with neuropathic pain after SNI have suffered from poor quality of life due to anxiety, depression and severe pain ( Bernetti et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of treadmill exercise on pain-related Wnt/β-catenin signaling pathway in dorsal root ganglion neurons at the early phase regeneration of the injured sciatic nerve

Cho¹,

Kim²,

Seo³

2021

J Exerc Rehabil

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The purpose of this study was to determine whether treadmill walking exercise can improve mechanical allodynia through regulation of Wnt/ β-catenin signaling in dorsal root ganglion (DRG) neurons at the early stage of regeneration after sciatic nerve injury (SNI). The experimental rats were divided into seven groups: the normal control, sedentary groups for 3-, 7-, and 14-day post crush (dpc), and exercise group for 3, 7, and 14 dpc. The rats in exercise groups performed treadmill walking exercise at a speed of 8 m/min for 20 min once a day according to experiment duration. For evaluating neuropathic pain-like behavior after SNI, the mechanical allodynia was examined by von Frey apparatus. And the expression levels of pain-related protein were identified in the cytoplasm or nucleus of DRG neurons using Western blot techniques. Mechanical allodynia was significantly ameliorated in the exercise group at 7 and 14 dpc. Treadmill exercise further decreased Wnt3a expression at 3, 7, and 14 dpc compared to in the sedentary group. Also, phosphorylated-low-density lipoprotein receptor 6 was decreased in exercise groups at 3 and 14 dpc. Beta-catenin was significantly decreased in exercise groups at 3 and 14 dpc compared to sedentary groups as well as treadmill exercise decreased translocation of β-catenin towards the nucleus of DRG neurons at 14 dpc. Our findings indicate that treadmill walking exercise may be an important regulator of neuropathic pain after peripheral nerve injury through delayed Wnt/ β-catenin signaling pathway in DRG neurons.

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Wrapping With Basic Fibroblast Growth Factor‐Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia

Cited by 11 publications

References 30 publications

Frozen Vein Wrapping for Chronic Nerve Constriction Injury Reduces Sciatic Nerve Allodynia in a Rat Model

Frozen Vein Wrapping for Chronic Nerve Constriction Injury Reduces Sciatic Nerve Allodynia in a Rat Model

Frozen vein wrapping for chronic nerve constriction injury reduces sciatic nerve allodynia in a rat model

Effect of treadmill exercise on pain-related Wnt/β-catenin signaling pathway in dorsal root ganglion neurons at the early phase regeneration of the injured sciatic nerve

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