WSG, a Glucose-Rich Polysaccharide from Ganoderma lucidum, Combined with Cisplatin Potentiates Inhibition of Lung Cancer In Vitro and In Vivo

Qiu, Wei-Lun; Hsu, Wen Lin; Tsao, Shu-Ming; Tseng, Ai-Jung; Zhang, Lin; Hua, Wei-Jyun; Yeh, Hung‐Chieh; Lin, Tzu‐En; Chen, Chien‐Chang; Chen, Lisheng; Lin, Tsai-Yun

doi:10.3390/polym13244353

Cited by 17 publications

(12 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The contributions of immune cells in modulating lung cancer metastatic processes through i.v. transplantation approaches have however remained limited to the syngeneic squamous Lewis lung cancer model ( Qiu et al, 2021 ), excluding other NSCLC histopathologies. To study the metastatic capability of histopathology-selective NSCLC cells, bulk cell populations from KL;ASC and KL;AC GEMM tumors were i.v.…”

Section: Resultsmentioning

confidence: 99%

Development of an adenosquamous carcinoma histopathology – selective lung metastasis model

et al. 2022

View full text Add to dashboard Cite

Preclinical tumor models with native tissue microenvironments provide essential tools to understand how heterogeneous tumor phenotypes relate to drug response. Here, we present syngeneic graft models of aggressive, metastasis-prone histopathology-specific NSCLC tumor types driven by KRAS mutation and loss of LKB1 (KL): adenosquamous carcinoma (ASC) and adenocarcinoma (AC). We show that subcutaneous injection of primary KL-ASC cells results in squamous cell carcinoma (SCC) tumors with high levels of stromal infiltrates, lacking the source heterogeneous histotype. Despite forming subcutaneous tumors, intravenously injected KL-AC cells were unable to form lung tumors. In contrast, intravenous injection of KL-ASC cells leads to their lung re-colonization and lesions recapitulating the mixed AC and SCC histopathology, tumor immune suppressive microenvironment and oncogenic signaling profile of source tumors, demonstrating histopathology-selective phenotypic dominance over genetic drivers. Pan-ERBB inhibition increased survival, while selective ERBB1/EGFR inhibition did not, suggesting a role of ERBB network crosstalk in resistance to ERBB1/EGFR. This immunocompetent NSCLC lung colonization model hence phenocopies key properties of the metastasis-prone ASC histopathology, and serves as a preclinical model to dissect therapy responses and metastasis-associated processes.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of an adenosquamous carcinoma histopathology – selective lung metastasis model

et al. 2022

View full text Add to dashboard Cite

show abstract

“… 2016 ) and suppress lung cancer (Qiu et al. 2021 ). However, not many studies have looked at the anti-inflammatory properties of GLP in acute pneumonia.…”

Section: Discussionmentioning

confidence: 99%

“… 2020 ; Qiu et al. 2021 ). However, so far, no research regarding the effect of GLP on acute pneumonia has been reported.…”

Section: Introductionmentioning

confidence: 99%

Ganoderma lucidum polysaccharides ameliorate lipopolysaccharide-induced acute pneumonia via inhibiting NRP1-mediated inflammation

Zhang

Tian

et al. 2022

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Ganoderma lucidum polysaccharides (GLP), from Ganoderma lucidum (Leyss. ex Fr.) Karst. (Ganodermataceae), are reported to have anti-inflammatory effects, including anti-neuroinflammation and anti-colitis. Nevertheless, the role of GLP in acute pneumonia is unknown. Objective To explore the protective role of GLP against LPS-induced acute pneumonia and investigate possible mechanisms. Materials and methods GLP were extracted and used for high-performance liquid chromatography (HPLC) analysis after acid hydrolysis and PMP derivatization. Sixty C57BL/6N male mice were randomly divided into six groups: Sham, Model, LPS + GLP (25, 50 and 100 mg/kg/d administered intragastrically for two weeks) and LPS + dexamethasone (6 mg/kg/d injected intraperitoneally for one week). Acute pneumonia mouse models were established by intratracheal injection of LPS. Haematoxylin and eosin (H&E) staining was examined to evaluate lung lesions. ELISA and quantitative real-time PCR were employed to assess inflammatory factors expression. Western blots were carried out to measure Neuropilin-1 expression and proteins related to apoptosis and autophagy. Results GLP suppressed inflammatory cell infiltration. In BALF, cell counts were 1.1 × 10 6 (model) and 7.1 × 10 5 (100 mg/kg). Release of GM-CSF and IL-6 was reduced with GLP (25, 50 and 100 mg/kg) treatment. The expression of genes IL-1β, IL-6, TNF-α and Saa3 was reduced. GLP treatment also suppressed the activation of Neuropilin-1 (NRP1), upregulated the levels of Bcl2/Bax and LC3 and led to downregulation of the ratio C-Caspase 3/Caspase 3 and P62 expression. Discussion and conclusions GLP could protect against LPS-induced acute pneumonia through multiple mechanisms: blocking the infiltration of inflammatory cells, inhibiting cytokine secretion, suppressing NRP1 activation and regulating pneumonocyte apoptosis and autophagy.

show abstract

“…It is known that cisplatin-based chemotherapy is one of the most prevalent treatments for lung cancer. A recent study revealed that the combined administration of WSG and cisplatin exerted synergistic inhibitory effects on the growth and metastasis of lung cancer in vitro and in vivo ( Qiu et al, 2021 ). WSG potentiated cisplatin-induced apoptotic responses in lung cancer cells but alleviated its cytotoxicity toward macrophages and normal lung fibroblasts.…”

Section: The Antitumor Activities Of Polysaccharides From G...mentioning

confidence: 99%

Research Progress on the Anticancer Activities and Mechanisms of Polysaccharides From Ganoderma

Wang

2022

Front. Pharmacol.

View full text Add to dashboard Cite

Cancer ranks as a primary reason for death worldwide. Conventional anticancer therapies can cause severe side effects, and thus natural products may be promising drug candidates for cancer therapy. Accumulating evidence has verified the prominent anticancer properties of Ganoderma polysaccharides, suggesting that Ganoderma polysaccharides may be effective chemopreventive agents of natural origin. Based on their abilities to prevent cancer development by regulating the DNA damage response, cancer cell proliferation, apoptosis, host immunity, gut microbiota and therapeutic sensitivity, there has been increasing interest in elucidating the clinical implication of Ganoderma polysaccharides in cancer therapy. In this review, we summarize recent findings pertaining to the roles of bioactive polysaccharides from Ganoderma in cancer pathogenesis, discuss the multifarious mechanisms involved and propose future directions for research. A more sophisticated understanding of the anticancer benefits of Ganoderma polysaccharides will be helpful for improving current treatments and developing novel therapeutic interventions for human malignancies.

show abstract

WSG, a Glucose-Rich Polysaccharide from Ganoderma lucidum, Combined with Cisplatin Potentiates Inhibition of Lung Cancer In Vitro and In Vivo

Cited by 17 publications

References 44 publications

Development of an adenosquamous carcinoma histopathology – selective lung metastasis model

Development of an adenosquamous carcinoma histopathology – selective lung metastasis model

Ganoderma lucidum polysaccharides ameliorate lipopolysaccharide-induced acute pneumonia via inhibiting NRP1-mediated inflammation

Research Progress on the Anticancer Activities and Mechanisms of Polysaccharides From Ganoderma

Contact Info

Product

Resources

About