Wuliangye Baijiu but not ethanol reduces cardiovascular disease risks in a zebrafish thrombosis model

Abstract

Understanding how Baijiu facilitates blood circulation and prevents blood stasis is crucial for revealing the mechanism of Baijiu for cardiovascular disease (CVD) risk reduction. Here we established a zebrafish thrombosis model induced using arachidonic acid (AA) to quantitatively evaluate the antithrombotic effect of Wuliangye Baijiu. The prevention and reduction effects of aspirin, Wuliangye, and ethanol on thrombosis were compared using imaging and molecular characterization. Wuliangye Baijiu reduces thrombotic risks and oxidative stress in the AA-treated zebrafish, while ethanol with the same concentration has no similar effect. The prevention and reduction effects of Wuliangye on thrombosis are attributed to the change in the metabolic and signaling pathways related to platelet aggregation and adhesion, oxidative stress and inflammatory response.

The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model

“…Therefore, in order to eliminate the influence of the solvent on the experimental results, a 5‰ DMSO equilibrium experiment variable was added to the blank group. The culture plate was placed into the incubator at 28°C for 6 h. Then, we added AA to all the experimental groups except for the blank group until the final concentration was 80 μM ( Ma et al, 2021 ; Zhu et al, 2022 ) and cultured for 1.5 h. In this experiment, each group involved three replicate wells, and each experiment was repeated three times.…”

“…Studies have shown that AA can directly activate platelets, induce platelet aggregation, and form thrombi. AA-induced thrombosis is thought to be representative of both venous and arterial thrombi ( Jagadeeswaran et al, 2016 ; Zhu et al, 2022 ). Therefore, in the present study, we investigated the active components and mechanisms of TP using AA to generate a zebrafish model of thrombosis.…”

Hypolipidemic and Antithrombotic Effect of 6′-O-Caffeoylarbutin from Vaccinium dunalianum Based on Zebrafish Model, Network Pharmacology, and Molecular Docking

“…When the number of cardiac erythrocytes in zebrafish heart is observed to be significantly lower than that in normal zebrafish via erythrocyte specific staining, this suggests that the zebrafish thrombus model is successfully constructed [ 47 ]. In recent years, the above methods for constructing zebrafish hyperlipidemia and thrombosis models have been widely used in the research of hypolipidemic and antithrombotic functions for natural products [ 48 , 49 , 50 ].…”

Zebrafish as a Model for Cardiovascular and Metabolic Disease: The Future of Precision Medicine

“…The Zebrafish model has the potential to facilitate these gene discovery advancements and thus can be utilized for functional validation and therapy-based studies to address thrombus formation. Recently, Zhu et al have shown that Wuliangye prevents and reduces thrombosis via changing metabolic and signaling pathways associated with platelet aggregation and adhesion, oxidative stress, and the inflammatory response [ 105 ].…”