Wuyiencin produced by Streptomyces albulus CK–15 displays biocontrol activities against cucumber powdery mildew

Abstract

Aims: Wuyiencin is a nucleoside antibiotic produced by Streptomyces albulus CK-15. The aim of this study was to determine whether wuyiencin can be used, as a suitable alternative to chemical pesticides, to protect cucumbers (Cucumis sativus L.) from powdery mildew caused by Sphaerotheca fuliginea. Further, the mechanisms underlying the control of cucumber powdery mildew by S. albulus CK-15 were preliminarily elucidated. Methods and Results: Wuyiencin solutions of different concentrations were used to treat infected cucumber plants under greenhouse conditions. The results indicated that wuyiencin could significantly reduce powdery mildew disease incidence, with a maximum prevention efficacy of 94Á38%. Further, scanning electron micrographs and enzyme assays showed that wuyiencin inhibited S. fuliginea spore growth and elicited the activity of plant systemic resistance-related enzymes. Additionally, real-time quantitative reverse transcription PCR suggested that wuyiencin can activate a salicylic aciddependent plant defence response. Conclusions: Wuyiencin produced by S. albulus CK-15 possessed antifungal effects and was able to mitigate cucumber powdery mildew disease via antagonistic action. Wuyiencin also induced defence responses in the plants. Significance and Impact of the Study: These results reinforce the biotechnological potential of wuyiencin as both an antagonistic agent and an inducer of plant systemic resistance.

Trichoderma-induced restructuring of the cucumber rhizosphere microbiome and the effect of a synthetic, cross-kingdom microbial community on Fusarium wilt disease and growth in cucumber

“…Speci cally, the application of FJ035 signi cantly increased the abundance of Stenotrophomonas and Streptomyces. These two genera are commonly recognized as Plant Growth Promoting Rhizobacteria (PGPR) 37 , and have been shown to effectively control some common cucumber diseases, such as Phytophthora crown rot 38 , anthracnose 39 , powdery mildew 40 and Fusarium wilt 37 . Previous studies have reported that the presence of Trichoderma spp.…”

A Rhizobacterium, Streptomyces albulus Z1-04-02, Displays Antifungal Activity against Sclerotium Rot in Mungbean

“…Streptomyces palmae PC12 showed strong antifungal activities against rice blast disease fungi and increased plant growth [ 39 ]. Recently, wuyiencin, a nucleoside antibiotic producing S. albulus CK-15, has been reported to be effective against cucumber powdery mildew [ 40 ]. The results from our study are in agreement with previous studies, as S. albulus Z1-04-02 exhibits diverse fungicidal activity against the growth of S. rolfsii and reduces the disease severity of stem rot in mungbeans.…”

Field Application of Wuyiencin Against Sclerotinia Stem Rot in Soybean

“…Field application technologies for cucumber powdery mildew, tomato gray mold, tomato leaf mold, and other fungal diseases have achieved great protection effects. Yang et al (2021a) reported that wuyiencin could significantly reduce powdery mildew disease incidence in cucumber, with a maximum prevention efficacy of 94.38%. In addition, wuyiencin has been applied in many provinces and cities in China, and it has a strong protection effect on S. sclerotiorum in Brassica napus (Zeng and Shi, 2003).…”

“…wuyiensis, a strain first isolated from a natural soil habitat in the Wuyi Mountains of China (Wei et al, 1984;Liu et al, 2018). This highly effective, broadspectrum, low-toxicity bio-pesticide is mainly used to control a variety of fungal diseases of vegetables, fruits, grains and other crops (Ge et al, 2015;Yang et al, 2021a). In a previous study, we observed that Wuyiencin protects soybean from S. sclerotiorum through antibiosis (inhibition of sclerotia production and germination), but application technologies for controling SSR using Wuyiencin have not been established (Yang et al, 2022).…”