Wuzi-Yanzong prescription alleviates spermatogenesis disorder induced by heat stress dependent on Akt, NF-κB signaling pathway

Hu, Su-Qin; Liu, Dian-Long; Li, Chunrui; Xu, Yang; Hu, Ke; Cui, Lidan; Guo, Jian

doi:10.1038/s41598-021-98036-2

Cited by 7 publications

(6 citation statements)

References 100 publications

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“…Testicular heat stress lowered serum testosterone levels, whereas the levels of follicle-stimulating hormone and luteinizing hormone were not significantly changed. The ultrastructure of the seminiferous tubules became abnormal after testicular heat stress, the structure of the blood–testis barrier became loose, and the Sertoli cells showed a trend of differentiation [ 48 ]. Heat stress decreased epithelial thickness, the loosening and vacuolization of the germinal epithelium, the presence of cellular debris in the lumen, and the appearance of multinucleated giant cells.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Curculigo orchioides Gaertn. Extract on Heat Stress-Induced Spermatogenesis Complications in Murine Model

Bui-Le

Quảng

Hoang-Viet

et al. 2023

CIMB

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Curculigo orchioides Gaertn. is a precious herb used in traditional medicine systems in Asian countries for various health benefits. This study investigated the potential protective effects of C. orchioides extract on reproductive health under heat stress conditions in male mice. Forty-eight mice were divided into eight groups, control condition (C group), C. orchioides extract at the dosages of 100, 200, and 400 mg/kg/day (C100, C200, C400 group), 40 °C heat exposure (H group), and combined 40 °C heat exposure and C. orchioides extract at the dosages of 100, 200, and 400 mg/kg/day (HC100, HC200, HC400 group). The result shows that the mice that received only C. orchioides extract without heat stress do not have a significant change in histological structure and testosterone level. The histological analysis of testicular tissue showed that heat stress conditions reduced reproductive function and inhibited the spermatogenesis of male mice. The C. orchioides rhizome extract treatment attenuated the heat stress-induced spermatogenesis complications in the murine model. Mice in the heat-stress group treated with C. orchioides extract had increased spermatogenic cells and spermatozoa compared with mice exposed to heat without C. orchioides treatment. Moreover, the aqueous extract of C. orchioides rhizome enhanced the serum total testosterone levels in heat-exposed mice. In conclusion, the study findings validate that C. orchioides is effective against heat stress-induced spermatogenesis complications in the murine model.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Curculigo orchioides Gaertn. Extract on Heat Stress-Induced Spermatogenesis Complications in Murine Model

Bui-Le

Quảng

Hoang-Viet

et al. 2023

CIMB

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“…The balance between germ cell proliferation and apoptosis is critical for spermatogenesis. It has previously been shown that heat‐stress or testicular hyperthermia decreases germ cell proliferation and induces apoptosis in the testis (Kanter et al, 2013; Hu et al, 2021; Hasani et al, 2020; Eslami Farsani et al, 2022). Our data are in agreement with a previous study, where it has been shown that heat stress induces apoptosis by stimulating active caspase3 expression, decreasing the survival factor Bcl2 (Vera et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a single dose of intra‐testicular insulin treatment in heat‐stressed mice model

Jeremy¹,

Gurusubramanian

Roy

2022

Andrologia

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Insulin plays important role in testicular functions such as germ cell proliferation and steroidogenesis, despite its conventional role as a hypoglycaemic agent. It is also well known that testicular activity is severely get affected by heat stress and heat stress induces testicular pathogenesis. The effect of insulin on heat‐induced testicular impairment has not been investigated. Thus, it is hypothesized that insulin might modulate testicular activity in a heat‐stressed model. Experimental mice were separated into 4 groups; the first group was the normal control (CN), and the second group was subjected to heat stress (HS) by submerging the lower body part in a thermostatically controlled water bath maintained at 43°C for 15 min. The third and fourth groups were treated with a single dose of intra‐testicular insulin (0.6 IU/mice) before and after heat stress. Animal tissue samples were collected after 14 days of heat treatment. Insulin treatment did not improve the sperm parameters; however, both insulin pre and post‐treatment improved the markers of spermatogenesis such as Johnsen score, germinal epithelium height and the number of stages VII/VIII. The histoarchitecture of testis also showed amelioration from heat‐induced pathogenesis in the insulin‐treated groups. Insulin treatment has also increased the proliferation of germ cells (increased PCNA and GCN), survival (Bcl2), and decreased apoptosis (active caspase‐3). Furthermore, insulin treatment decreased MDA levels, without pronounced effects on the activities of antioxidant enzymes. Heat stress also decreased the circulating testosterone and oestrogen levels, and insulin treatment significantly increased oestrogen levels only. Although testosterone showed an increasing trend, it was insignificant. The expression of aromatase, AR, ER‐α, and ER‐β was down regulated by heat‐stress and insulin treatment up regulated these markers. In conclusion, our results showed the amelioration of heat‐induced testicular impairment by pre and post‐intra‐testicular insulin treatments. Insulin‐associated improvements in the pre‐and post‐treatment groups suggested a preventive mechanism of insulin against heat stress in the testis.

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“…This regulation, in turn, is achieved through the effect of WZYZP on the levels of T, AR, erythropoietin (EPO), and Tyro-3, Axl, and Mer receptors. [60] Li [66] found that WZYZP could stimulate the proliferation of juvenile rat testis-supporting cells by activating the PI3K/Akt signaling pathway and prompting Akt phosphorylation. Chen et al [49] showed that WZYZP suppressed TGF-β expression and activated the PI3K/Akt/mTOR signaling pathway, thereby promoting germ cell proliferation and inhibiting germ cell apoptosis and autophagy.…”

Section: Modulatory Effects On Signaling Pathwaysmentioning

confidence: 99%

“…Chen et al [49] showed that WZYZP suppressed TGF-β expression and activated the PI3K/Akt/mTOR signaling pathway, thereby promoting germ cell proliferation and inhibiting germ cell apoptosis and autophagy. Hu et al [60] affirmed that WZYZP could restore BTB structural function, inhibit inflammatory factor expression, and reduce tissue cell damage in testicular inflammatory rats by blocking the TLR/MyD88/NF-κB pathway. Yang et al [54] illustrated that WZYZP regulated testicular spermatogenesis in rats with oligoasthenozoospermia through the TGF-β1/ Smads signaling pathway.…”

Section: Modulatory Effects On Signaling Pathwaysmentioning

confidence: 99%

Research progress on Wuzi Yanzong pills in the treatment of male infertility

Ip,

Hong,

Tong

et al. 2023

Sci Tradit Chin Med

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In recent years, because of changes in lifestyle, social environments, and other factors, male fertility has exhibited a declining trend in various countries and regions, including China. This poses a challenge for the prevention and treatment of male infertility. Wuzi Yanzong pill (WZYZP) is a renowned Chinese medicinal formula utilized for the treatment of male infertility and boasts a long history of use in this context. This article summarized the treatment approaches used in both Chinese and Western medicine for male infertility, along with the research advancements pertaining to the analysis of Chinese medical theory, pharmacological effects, bioactive ingredients, and the establishment of quality control standards for WZYZP in the treatment of male infertility, aiming to provide references for research related to the mechanism of action, identification of active ingredients, and clinical application of WZYZP in the treatment of male infertility.

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Wuzi-Yanzong prescription alleviates spermatogenesis disorder induced by heat stress dependent on Akt, NF-κB signaling pathway

Cited by 7 publications

References 100 publications

Protective Effect of Curculigo orchioides Gaertn. Extract on Heat Stress-Induced Spermatogenesis Complications in Murine Model

Protective Effect of Curculigo orchioides Gaertn. Extract on Heat Stress-Induced Spermatogenesis Complications in Murine Model

Evaluation of a single dose of intra‐testicular insulin treatment in heat‐stressed mice model

Research progress on Wuzi Yanzong pills in the treatment of male infertility

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