X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes

Marchal, Juan A.; Acosta, Manuel J.; Nietzel, H.; Sperling, Karl; Bullejos, Mónica; Guardia, R. Díaz de la; Sánchez, Antonio

doi:10.1007/s10577-005-5077-0

Cited by 48 publications

(69 citation statements)

References 39 publications

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“…It is unlikely that these two mechanisms of ampli¢ca-tion took place during the evolution of the sex chromosomes of M. cabrerae, since the composition of the heterochromatin of both sex chromosomes is di¡erent. In fact, di¡erences in sequence composition between the X and Y heterochromatic blocks of M. cabrerae have been revealed by FISH with MSAT-160 satellite DNA (Ferna¤ ndez et al 2001) or with SRY gene (Ferna¤ ndez et al 2002), by X chromosome painting (Marchal et al 2004) and in this study. Other ampli¢cation mechanisms, such as sister chromatid exchanges or replication slippage, could also explain the heterochromatin ampli¢cation on the sex chromosomes of Microtus.…”

Section: Resultssupporting

confidence: 57%

A repeat DNA sequence from the Y chromosome in species of the genus Microtus

2004

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In most mammals, the Y chromosome is composed of a large amount of constitutive heterochromatin. In some Microtus species, this feature is also extended to the X chromosome, resulting in enlarged (giant) sex chromosomes. Several repeated DNA sequences have been described in the gonosomal heterochromatin of these species, indicating that it has heterogeneous and species-speci¢c composition and distribution. We have cloned an AT-rich, 851-bp long, repeated DNA sequence speci¢c for M. cabrerae Y chromosome heterochromatin. The analysis of other species of the genus Microtus indicated that this sequence is also located on the Y chromosome (male-speci¢c) in three species (M. agrestis, M. oeconomus and M. nivalis), present on both Y and X chromosomes and on some autosomes in M. arvalis and absent in the genome of M. guentheri. Our data also suggest that the mechanism of heterochromatin ampli¢cation operating on the sex chromosomes could have been different in each species since the repeated sequences of the gonosomal heterochromatic blocks in M. cabrerae and M. agrestis are different. The absence of this sequence in the mouse genome indicates that its evolutionary origin could be recent. Future analysis of the species distribution, localization and sequence of this repeat DNA family in arvicolid rodent species could help to establish the unsolved phylogenetic relationships in this rodent group.

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Section: Resultssupporting

confidence: 57%

A repeat DNA sequence from the Y chromosome in species of the genus Microtus

2004

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“…When the DNA was amplified by this last method, we immediately reamplified it, following supplier instructions, and then marked it by nick translation with rhodamine-11-dUTP or fluorogreen-11-dUTP. When the DNA was amplified by DOP-PCR, we re-amplified and marked DNA with SpectrumOrange-dUTP (Vysis) [Marchal et al, 2004].…”

Section: Methodsmentioning

confidence: 99%

“…Chromosome painting was performed as described in Marchal et al [2004] on mitotic metaphase cells from 1 embryo obtained Teruel /Cabrero /Perfectti /Acosta / Sánchez /Camacho Cytogenet Genome Res 2009;125:286-291 288 in our laboratory carrying the B 1 (from the Mundo river population, Albacete, Spain) and 2 embryos carrying B 24 (from Torrox) chromosomes. In addition, primary spermatocytes from a male collected at Goynuk (Turkey) were submitted to chromosome painting.…”

Section: Methodsmentioning

confidence: 99%

Microdissection and Chromosome Painting of X and B Chromosomes in the Grasshopper <i>Eyprepocnemis plorans</i>

Teruel

Cabrero

Perfectti

et al. 2009

Cytogenet Genome Res

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The relative location of 2 repetitive DNAs, i.e. ribosomal (rDNA) and a tandemly repeated satellite DNA (satDNA), with respect to the centromere, suggested that B chromosomes in the grasshopper Eyprepocnemis plorans derived intraspecifically from the X chromosome. To test this hypothesis, we microdissected X and B chromosomes and amplified the obtained DNA by 2 different procedures, the conventional DOP-PCR method and the single-cell whole-genome amplification GenomePlex® method. We then generated DNA probes to perform chromosome painting. Our results have confirmed that X and B chromosomes share many DNA sequences between them and with most of the autosomes, especially at locations where the satDNA and rDNA reside, in consistency with previous information. This supports the hypothesis of an intraspecific origin of B chromosomes in E. plorans. Nevertheless, the present results did not help to clarify whether Bs were derived from the X chromosome or else from 1 or more autosomes.

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“…In a diversity of organisms, heterochromatinization, accompanied by amplification of tandem repeats, represents an important step in the morphological differentiation of simple sex chromosome systems, especially in the ZW ones (Nanda et al, 2000;Kondo et al, 2004;Marchal et al, 2004;Peichel et al, 2004;Charlesworth et al, 2005;Ezaz et al, 2009;Kejnovský et al, 2009). Same type of studies provided evidence that rDNA cistrons can also occur on the sex chromosomes of distinct organisms (see, for example, Goodpasture and Bloom, 1975;Schmid et al, 1983;Yonenaga-Yassuda et al, 1983;Morielle and Varella-Garcia, 1988;Cioffi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

et al. 2016

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The main objectives of this study were to test: (1) whether the W-chromosome differentiation matches to species' evolutionary divergence (phylogenetic concordance) and (2) whether sex chromosomes share a common ancestor within a congeneric group. The monophyletic genus Triportheus (Characiformes, Triportheidae) was the model group for this study. All species in this genus so far analyzed have ZW sex chromosome system, where the Z is always the largest chromosome of the karyotype, whereas the W chromosome is highly variable ranging from almost homomorphic to highly heteromorphic. We applied conventional and molecular cytogenetic approaches including C-banding, ribosomal DNA mapping, comparative genomic hybridization (CGH) and cross-species whole chromosome painting (WCP) to test our questions. We developed Z-and W-chromosome paints from T. auritus for cross-species WCP and performed CGH in a representative species (T. signatus) to decipher level of homologies and rates of differentiation of W chromosomes. Our study revealed that the ZW sex chromosome system had a common origin, showing highly conserved Z chromosomes and remarkably divergent W chromosomes. Notably, the W chromosomes have evolved to different shapes and sequence contents within~15-25 Myr of divergence time. Such differentiation highlights a dynamic process of W-chromosome evolution within congeneric species of Triportheus.

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X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes

Cited by 48 publications

References 39 publications

A repeat DNA sequence from the Y chromosome in species of the genus Microtus

A repeat DNA sequence from the Y chromosome in species of the genus Microtus

Microdissection and Chromosome Painting of X and B Chromosomes in the Grasshopper <i>Eyprepocnemis plorans</i>

Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

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