X-linked mutations that give rise to overproduction of glucose-6-phosphate dehydrogenase in Drosophila melanogaster

Iwabuchi, Masaki; Hori, Samuel H.; Yorimoto, Naoki

doi:10.1007/bf00502798

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…1983;Fox, 1971;Stewart and Merriam, 1974), each encoding one of the IDH subunits. The Zwischenferment locus (Zw; 1-62.9) is thought to encode the major polypeptide of the first pentose phosphate shunt enzyme, glueose-6-phosphate dehydrogenase [G6PD; EC 1.1.1.49 (Eanes, 1983;Iwabuchi et al, 1986)]. Pgd (1-0.64) is thought to encode the major polypeptide of 6-phosphogluconate dehydrogenase [6PGD; EC 1.1.1.44 (Bewley and Lucchesi, 1975;Gerasimova and Ananiev, 1972;Gvozdev et al, 1977;Young, 1966)].…”

Section: Introductionmentioning

confidence: 99%

The isolation and characterization of a mutant allele at a new X-linked locus,mex, affecting NADP+-dependent enzymes inDrosophila melanogaster

Gromnicki

Bentley

1991

Biochem Genet

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The isolation and characterization of mutant alleles in a regulatory gene affecting NADP(+)-dependent enzymes are described. The locus, mex, is at position 26.5 +/- 0.74 on the X chromosome of Drosophila melanogaster. The newly isolated mutant allele, mex1, is recessive to either the mex allele found in Oregon-R wild-type individuals or that found in the cm v parental stock in which the new mutants were induced. The mex1 mutant allele is associated with statistically significant decreases in malic enzyme (ME) specific activity and ME specific immunologically cross-reacting material (ME-CRM) in newly emerged adult males. During this same developmental stage in males, the NADP(+)-dependent isocitrate dehydrogenase specific activity increases to statistically significant levels. Females of the mex1 mutant strain show statistically significant elevated levels of the pentose phosphate shunt enzymes, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Isoelectric focusing and thermolability comparisons of the active ME from mutant and control organisms indicate that the enzyme is the same. Developmental profiles of mex1 and control strains indicate that this mutant allele differentially modulates the levels of ME enzymatic activity and ME-CRM during development.

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Section: Introductionmentioning

confidence: 99%

The isolation and characterization of a mutant allele at a new X-linked locus,mex, affecting NADP+-dependent enzymes inDrosophila melanogaster

Gromnicki

Bentley

1991

Biochem Genet

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“…These mutations are all X linked and cis dominant and appear to be regulatory mutations, since the copy number of the G6PD gene is the same in wild-type and mutant flies (Iwabuchi et al, 1986). Close linkage of the regulatory factor to the G6PD gene has also been demonstrated by mapping experiments (Iwabuchi et al, 1986). Interestingly, these mutants often revert to wild-type upon outerossing, and the reversion rate depends on the direction of the cross, being analogous to hybrid dysgenesis (Tanda and Hori, 1983a;Itoh and Hori, 1985).…”

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 94%

“…One of the isogenic strains (1F) thus established was bidirectionally selected for G6PD activity, and high (1FH) and low (1FL) strains were obtained (Iwabuchi et al, 1986). $44H and $44L.…”

Section: Fh and Iflmentioning

confidence: 99%

“…In the course of studies on genetic factors affecting glucose-6-phosphate dehydrogenase (G6PD) gene expression in D. melanogaster, three mutations which give rise to overproduction of G6PD have been found among flies subjected to artificial selection for G6PD activity (Tanda and Hori, 1983a, b;Iwabuchi et al, 1986;Itoh et al, 1988). These mutations are all X linked and cis dominant and appear to be regulatory mutations, since the copy number of the G6PD gene is the same in wild-type and mutant flies (Iwabuchi et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Positive regulation of theDrosophila melanogaster G6PD gene by an insertion sequence

1989

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In a previous study, we have shown that the three high-G6PD activity mutants are characterized by insertion of the Ins1 sequence consisting of a core sequence flanked by two defective P elements (KP and KP'; the 32nd base of the KP was replaced by guanine in the KP') in front of exonI of the G6PD gene and that the sequence responsible for positive regulation of the G6PD gene expression might be the core sequence but not the flanking KP and KP' elements. The core sequence is composed of either one or two identical units in each mutant. In this report we present evidence (1) that insertion of the Ins1 sequence gives rise to overproduction of G6PD mRNA, (2) that the length and the 5' end of G6PD mRNA do not differ in wild-type and three mutants, (3) that the insertion site of the Ins1 sequence is the same in the mutants, and (4) that each unit of the core sequence has the same in the mutants, and (4) that each unit of the core sequence has a pair of DNase I-hypersensitive sites. The possibility exists that the binding of some regulatory proteins to the DNase I-hypersensitive sites might accelerate the transcription rate of the G6PD gene.

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Transcriptional activation of the Drosophila melanogaster glucose-6-phosphate dehydrogenase gene by insertion of defective P elements

1993

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Tandem insertions of defective P elements (1.15 kb KP and 0.6 kb core P) accelerate the transcription rate of the glucose-6-phosphate dehydrogenase (G6PD) gene in Drosophila melanogaster. In this report, we have analyzed the activation mechanism of the G6PD promoter by in vitro transcription and gel retardation assays. Results showed that one cis-acting region in the core P and two such regions in the KP are associated with activation of the G6PD promoter, and that putative transcriptional regulatory protein(s) which specifically bind to each of the cis-acting regions are present in nuclear extracts of Canton S embryos. On the other hand, the P elements do not activate the normal actin 5C promoter, but activate the promoter when the 20 bp sequence around the G6PD transcription start site is placed in front of the promoter. It appears that the GC-rich region in this 20 bp sequence is required for the activation.

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X-linked mutations that give rise to overproduction of glucose-6-phosphate dehydrogenase in Drosophila melanogaster

Cited by 8 publications

References 13 publications

The isolation and characterization of a mutant allele at a new X-linked locus,mex, affecting NADP+-dependent enzymes inDrosophila melanogaster

The isolation and characterization of a mutant allele at a new X-linked locus,mex, affecting NADP+-dependent enzymes inDrosophila melanogaster

Positive regulation of theDrosophila melanogaster G6PD gene by an insertion sequence

Transcriptional activation of the Drosophila melanogaster glucose-6-phosphate dehydrogenase gene by insertion of defective P elements

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