X-ray ablation of hyaluronan hydrogels: Fabrication of three-dimensional microchannel networks

Weon, Byung Mook; Chang, Shau-Feng; Yeom, Junseok; Hahn, Sei Kwang; Je, J. H.; Hwu, Y.; Margaritondo, G.

doi:10.1063/1.3213358

Cited by 6 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…21 Besides, fabrication routes such as X-ray ablation, layer-by-layer printing were also used for 3D microstructure prototyping. 22 Recently, a novel self-folding route was reported for fabrication of real 3D microstructures from precreated 2D micropatterns. [23][24][25] Using self-assembly, 2D cruciform structures with porosity could be transformed into 3D microcontainers with porous walls, 26,27 which shows great potential for cell encapsulation and separation towards microfluidic appliations.…”

Section: Introductionmentioning

confidence: 99%

Embellishment of microfluidic devices via femtosecond laser micronanofabrication for chip functionalization

Wang

Xia

et al. 2010

Lab Chip

View full text Add to dashboard Cite

This paper demonstrates the embellishment of existing microfluidic devices with integrated three dimensional (3D) micronanostructures via femtosecond laser micronanofabrication, which, for the first time, proves two-photon photopolymerization (TPP) to be a powerful technology for chip functionalization. As representative examples, microsieves with various pore shape and adjustable pore size were successfully fabricated inside a conventional glass-based microfluidic channel prepared by wet etching for microparticle separation. Moreover, a fish scale like microfilter was also fabricated and appointed as a one-way valve, which showed excellent performance as we expected. These results indicate that such embellishment of microfluidic devices is simple, low cost, flexible and easy to access. We believe that, combined with TPP, the application of lab-on-chip devices would be further extended.

show abstract

Section: Introductionmentioning

confidence: 99%

Embellishment of microfluidic devices via femtosecond laser micronanofabrication for chip functionalization

Wang

Xia

et al. 2010

Lab Chip

View full text Add to dashboard Cite

show abstract

“…[14] Here our results of PDMS demonstrate that X-ray irradiation is a highly efficient and feasible protocol for surface modification through PDMS ablation. The X-ray-induced ablation enables one not only to oxidize the PDMS surface (hydrophilicity) but also to deposit the ablated PDMS (hydrophobicity) on other hydrophilic surfaces.…”

mentioning

confidence: 78%

Ablation and Deposition of Poly(dimethylsiloxane) with X‐Rays

et al. 2010

View full text Add to dashboard Cite

Poly(dimethylsiloxane) [PDMS] is the most widely used siliconbased organic polymer due to its excellent mechanical, chemical, and optical properties for microfluidics, microchips, microlithography, electrophoresis, and biomedical applications. One challenge for the applications is to modify the surface wettability with long-term stability in air. Herein we present a highly efficient protocol for the surface modification of PDMS using Xray irradiation, which enables not only to "ablate" hydrophobic molecules from PDMS, rendering the surface hydrophilic in atmosphere and room temperature conditions, but also to "deposit" them on flat and nanostructure surfaces, inducing hydrophobic surfaces.In spite of the development of many interesting applications, [1][2][3][4] microfluidic devices based on PDMS are not yet standard equipment observed in most biologists' toolboxes. [5] One of the obstacles is the difficulty in making PDMS-based devices. For instance, soft lithography, the main fabrication process, requires using a clean room, [5] which makes the traditional soft lithography approach painfully slow and difficult. The intrinsic hydrophobicity of PDMS that comes from SiÀCH 3 end-bonds is another big problem in applying as an ideal flexible substrate for microfluidic channels. [3,4] The PDMS surface can be rendered hydrophilic using a variety of methods (see a review [4] ) such as oxygen plasmas, [6] corona discharges, [7] and ultraviolet lights, [8] and exposures of lasers or excimer lamps.[9] The hydrophilic property, however, quickly diminishes through the sequent hydrophobic recovery.[2, 4] Such physical protocols usually require an additional sophisticated chemical process to stabilize the hydrophilic SiO 2 layer.[4] Laser irradiation that enables one not only to modify, but also to ablate the PDMS surface through photothermal and photochemical effects [9c] may have a limitation in applications to organic polymers such as PDMS because of thermal damage. It is thus required to develop a versatile and robust protocol to modify and ablate polymers simultaneously with a finely tunable, nonthermal irradiation technique.Contrary to laser beams, ionizing radiation can induce photochemical scission of PDMS without thermal damage. For instance, electron irradiation, revealed by Miller as early as 1960, causes SiÀCH 3 and SiCH 2 ÀH scissions, generating H 2 , CH 4 , and C 2 H 6 as gaseous products. [10,11] Despite the well-established radiation chemistry of PDMS, there are few reports to demonstrate highly efficient wettability changes in PDMS using ionizing radiation.From studies on various soft matter and inorganic systems using hard X-rays (10-60 keV), [12,13] we recently developed an X-ray ablation technique using rapid depolymerization of organic polymers.[14] Here our results of PDMS demonstrate that X-ray irradiation is a highly efficient and feasible protocol for surface modification through PDMS ablation. The X-ray-induced ablation enables one not only to oxidize the PDMS surface (hydrophilicity) but also ...

show abstract

“…177 Such microchannels, embedded in a hyaluronan hydrogel, has also been by using hard x-ray irradiation. 178 In another approach, hydrogel mediated partitioning of microchannels has been used to create tunable 3-D cellular microenvironment. 179 To stretch the scope of applicability even further, a research group has recently reported a method to fabricate biofunctionalized polyethylene glycol hydrogel microchannels with adjustable circular cross-sections.…”

Section: Effect Of the Microconfinementmentioning

confidence: 99%

Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?

Das

Chakraborty

2013

Biomicrofluidics

View full text Add to dashboard Cite

According to the World Health Organization, cancer is one of the leading causes of death worldwide. Cancer research, in its all facets, is truly interdisciplinary in nature, cutting across the fields of fundamental and applied sciences, as well as biomedical engineering. In recent years, microfluidics has been applied successfully in cancer research. There remain, however, many elusive features of this disease, where microfluidic systems could throw new lights. In addition, some inherent features of microfluidic systems remain unexploited in cancer research. In this article, we first briefly review the advancement of microfluidics in cancer biology. We then describe the biophysical aspects of cancer and outline how microfluidic system could be useful in developing a deeper understanding on the underlying mechanisms. We next illustrate the effects of the confined environment of microchannel on cellular dynamics and argue that the tissue microconfinement could be a crucial facet in tumor development. Lastly, we attempt to highlight some of the most important problems in cancer biology, to inspire next level of microfluidic applications in cancer research.

show abstract

X-ray ablation of hyaluronan hydrogels: Fabrication of three-dimensional microchannel networks

Cited by 6 publications

References 30 publications

Embellishment of microfluidic devices via femtosecond laser micronanofabrication for chip functionalization

Embellishment of microfluidic devices via femtosecond laser micronanofabrication for chip functionalization

Ablation and Deposition of Poly(dimethylsiloxane) with X‐Rays

Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?

Contact Info

Product

Resources

About