X-ray and infrared study of Langmuir-Blodgett films of the complexes between nucleic acids and aliphatic amines

Sukhorukov, Gleb B.; Feĭgin, L. A.; Montrel, M.M.; Сухоруков, Б. И.

doi:10.1016/0040-6090(94)06414-8

Cited by 35 publications

(30 citation statements)

References 10 publications

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“…The assignment of these bands in Figure 2 is in accordance with previous studies on the characterization of DNA structure using FT-IR. 36,37 These results support the sequential assembly of mixed SAM, Fc-D, and thiolated capture probe DNA via the respective linkers described in Scheme 1.…”

Section: Resultssupporting

confidence: 76%

Enzyme-Amplified Electrochemical Detection of DNA Using Electrocatalysis of Ferrocenyl-Tethered Dendrimer

et al. 2003

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We have developed a sandwich-type enzyme-linked DNA sensor as a new electrochemical method to detect DNA hybridization. A partially ferrocenyl-tethered poly(amidoamine) dendrimer (Fc-D) was used as an electrocatalyst to enhance the electronic signals of DNA detection as well as a building block to immobilize capture probes. Fc-D was immobilized on a carboxylic acid-terminated self-assembled monolayer (SAM) by covalent coupling of unreacted amine in Fc-D to the acid. Thiolated capture probe was attached to the remaining amine groups of Fc-D on the SAM via a bifunctional linker. The target DNA was hybridized with the capture probe, and an extension in the DNA of the target was then hybridized with a biotinylated detection probe. Avidin-conjugated alkaline phosphatase was bound to the detection probe and allowed to generate the electroactive label, p-aminophenol, from p-aminophenyl phosphate enzymatically. p-Aminophenol diffuses into the Fc-D layer and is then electrocatalytically oxidized by the electronic mediation of the immobilized Fc-D, which leads to a great enhancement in signal. Consequently, the amount of hybridized target can be estimated using the intensity of electrocatalytic current. This DNA sensor exhibits a detection limit of 20 fmol. Our method was also successfully applied to the sequence-selective discrimination between perfectly matched and single-base mismatched target oligonucleotides.

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Section: Resultssupporting

confidence: 76%

Enzyme-Amplified Electrochemical Detection of DNA Using Electrocatalysis of Ferrocenyl-Tethered Dendrimer

et al. 2003

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“…The authors also demonstrated that the DODAB molecules are largely tilted relative to the normal of the interface at low surface pressure but progressively become less tilted as the surface pressure increases. Additionally, Sukhorukov et al [13] showed that double-stranded DNA adsorbs to cationic monolayers at the air-water interface without any denaturation into single-stranded DNA. The present report focuses on the interaction of doublestranded DNA with various lipid monolayers at the airwater interface and the results are discussed in relation to the charge density of the monolayer.…”

Section: Introductionmentioning

confidence: 98%

“…This is likely to involve the interaction between DNA at a lipid-aqueous interface. In the last decade, the potential use of DNA and cationic surfactants as vehicles for gene delivery and as biofunctional materials and biosensors have promoted the study of such systems not only in the bulk but also at the air-water interface [9][10][11][12][13]. Earlier Sun et al [10] investigated the effect of DNA on the morphology of the cationic monolayers from bromide dioctadecyldimethylammonium (DODAB) at the air-water interface by means of BAM.…”

Section: Introductionmentioning

confidence: 98%

The interaction between DNA and cationic lipid films at the air–water interface

Cárdenas

Nylander

Jönsson

et al. 2005

Journal of Colloid and Interface Science

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“…Special focus was put on the coupling of DNA with the monolayer resulting in a one-dimensional ordering of bio-macromolecules at the charged surface. Despite a large number of publications devoted to different aspects of DNA/cationic monolayer interaction, [12][13][14][15][16][17][18][19][20][21] the influence of the chemical structure of lipid molecules as well as of the monolayer phase state on the coupling behavior is not fully understood. Therefore, emphasis was placed on a detailed physical-chemical characterization of the novel amphiphilic compounds accompanied by biological testing.…”

Section: Introductionmentioning

confidence: 98%

Physical–Chemical Properties and Transfection Activity of Cationic Lipid/DNA Complexes

et al. 2009

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Investigation of DNA interactions with cationic lipids is of particular importance for the fabrication of biosensors and nanodevices. Furthermore, lipid/DNA complexes can be applied for direct delivery of DNA-based biopharmaceuticals to damaged cells as non-viral vectors. To obtain more effective and safer DNA vectors, the new cationic lipids 2-tetradecylhexadecanoic acid-{2-[(2-aminoethyl)amino]ethyl}amide (CI) and 2-tetradecylhexadecanoic acid-2-[bis(2-aminoethyl)amino]ethylamide (CII) were synthesized and characterized. The synthesis, physical-chemical properties and first transfection and toxicity experiments are reported. Special attention was focused on the capability of CI and CII to complex DNA at low and high subphase pH values. Langmuir monolayers at the air/water interface represent a well-defined model system to study the lipid/DNA complexes. Interactions and ordering of DNA under Langmuir monolayers of the new cationic lipids were studied using film balance measurements, grazing incidence X-ray diffraction (GIXD) and X-ray reflectivity (XR). The results obtained demonstrate the ability of these cationic lipids to couple with DNA at low as well as at high pH value. Moreover, the observed DNA structuring seems not to depend on subphase pH conditions. An influence of the chemical structure of the lipid head group on the DNA binding ability was clearly observed. Both compounds show good transfection efficacy and low toxicity in the in vitro experiments indicating that lipids with such structures are promising candidates for successful gene delivery systems.

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X-ray and infrared study of Langmuir-Blodgett films of the complexes between nucleic acids and aliphatic amines

Cited by 35 publications

References 10 publications

Enzyme-Amplified Electrochemical Detection of DNA Using Electrocatalysis of Ferrocenyl-Tethered Dendrimer

Enzyme-Amplified Electrochemical Detection of DNA Using Electrocatalysis of Ferrocenyl-Tethered Dendrimer

The interaction between DNA and cationic lipid films at the air–water interface

Physical–Chemical Properties and Transfection Activity of Cationic Lipid/DNA Complexes

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