“…This is important since a classical ground truth via root excavation and washing, results in a loss of fine root structure and interconnectivity of the whole root system. To this end, the 3D segmentation of the root structures was mainly performed by analytical algorithms based on classical image processing and image analysis methods [e.g., Mairhofer et al., 2011 ; Flavel et al., 2012 ; Mairhofer et al., 2015 ; Flavel et al., 2017 ; Gao et al., 2019 ; Soltaninejad et al., 2020 ; Gerth et al., 2021 ; Phalempin et al., 2021 ; Ferreira et al, 2022 ; Lucas & Vetterlein, 2022 ), which, however, are not able to detect roots on all scales equally. See Figure 1 for an example which contains challenging small and fine roots.…”