X-Ray Diffraction Studies of Lecithin Bimolecular Leaflets with Incorporated Fluorescent Probes

Lesslauer, Werner; Cain, J. E.; Blasie, J. Kent

doi:10.1073/pnas.69.6.1499

Cited by 127 publications

(90 citation statements)

References 11 publications

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“…The most reliable X-ray diffraction study of a bilayer in the gel state is probably that of Blasie and co-workers (Lesslauer, Cain & Blasie, 1972). Utilizing the surface deposition technique of Blodgett and Langmuir these authors prepared orientated multilayers of dipalmitoyl-3-w-phosphatidylcholine and examined the X-ray diffraction pattern in the range of relative humidities from 15 to 95%.…”

Section: Structure and Flexibility Of The Polar Head Groupsmentioning

confidence: 99%

Deuterium magnetic resonance: theory and application to lipid membranes

Seelig

1977

Quart. Rev. Biophys.

1,268

1,041

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Proton and carbon-13 nmr spectra of unsonicated lipid bilayers and biological membranes are generally dominated by strong proton–proton and proton–carbon dipolar interactions. As a result the spectra contain a large number of overlapping resonances and are rather difficult to analyse. Nevertheless, important information on the structure and dynamic behaviour of lipid systems has been provided by these techniques (Wennerström & Lindblom, 1977).

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Section: Structure and Flexibility Of The Polar Head Groupsmentioning

confidence: 99%

Deuterium magnetic resonance: theory and application to lipid membranes

Seelig

1977

Quart. Rev. Biophys.

1,268

1,041

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“…Accumulated evidence confirms the dominance of fluorescence from l -anilino-8-naphthalenesulphonate bound to lecithin-sphingomyelin pockets in natural membranes (19,20,21).…”

Section: Discussionmentioning

confidence: 48%

“…The binding of amphipathic molecules of 1-anilino-8-naphthalenesulphonate to lipid bilayers is assumed to involve insertion of the apolar naphthalene moiety into the hydrocarbön region, while the charged sulphonate moiety remains in the plane of the polar head groups (20,21,24). Thus, any enhanced lateral compressibility in the liquid crystalline state near the temperature of lipid phase transition (t^) could restilt in increased dye binding in this region, by allowing the insertion of l-anilino-S^naphthalenesulphonate into its membrane binding site with minimal perturbation (25).…”

Section: Discussionmentioning

confidence: 99%

1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane Dynamics?

Watała

Jóźwiak²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:In this study we report the use of l-anilino-8-naphthalenesulphonate äs a fluorescence probe to investigate the properties of plasma membranes derived from normal and diabetic red blood cells.The binding of l-anilino-8-naphthalenesulphonate to diabetes-affected erythrocyte membranes, äs compared with controls, was measured by means of fluorescence polarization and fluorescence titration techniques.These measurements demonstrated anomalous l-anilino-^S-naphthalenesulphonate binding to pathological red cell membranes. The amount of l-anilino-8-naphthalenesulphonate bound to diabetic erythrocyte membranes was greater than that of controls. This fluorometric study indicated that the outer monolayer binding of l-anilino-8-naphthalenesulphonate was markedly augmented in the erythrocyte membranes of diabetic patients. Significant correlations were found between l-anilino-8-naphthalenesulphonate binding parameters and membrane lipid composition. The correlation between these binding parameters and non-enzymatic protein glycation was poor or moderate.Though the fluorescence intensity and emission maximum were quite similar in both groups investigated, binding studies reveäled that there were approximately 1.2 times the number of l-anilino-8-naphthalenesulphonate binding sites and a 33% increase in the K O value in diabetic membranes, suggesting significant differences in the environment of the l-anilino-8-naphthalenesulphonate binding sites in these two groups of patients.The results presented in this report indicate that (a) l-aniliüo-8-naphthalenesulphonate is a sensitive probe of membrane architecture alterations, and can be used to elucidate the perturbating effects of sterols in membrane Systems, and (b) that significant differences in membrane dynamics exist between normal and diabetic red cell membranes.The possibility that the increase in bound dye in diabetics was caused by enhanced lateral compressibility, or deiisity flüctuations, of whether it was due to additional binding sites at the boundary of heterogeneous lipid chisters is discussed. troductiono f fluorescence spectroscopy has been The revival of interest in fluorescence probes in recent used to probe many parameters of membrane strucyears has resulted in numerous studies dealing with ture using a variety of extrinsic probe molecules (2, thepertinehcepfselectedflüorophoresformonitoring 3), and several cheinicals have been especially dechanges of membrane structure and dynamics (l, 2), signed for this purpose. Amongst them, l-anilino-8-

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“…Luzzati, Tardieu & Taupin (1972) used the lamellar reflexions up to the 9th and obtained the density profile with a resolution of about 6.7 A. Lesslauer, Cain & Blasie (1972) used the lamellar reflexions up to the 10th together with diffraction data from the dispersions and obtained the density profile with a resolution of about 6 A. The result of the former was given in the absolute scale and is reproduced by the dashed line in Fig.…”

Section: Discussionmentioning

confidence: 71%

Structures of uranyl-decorated lecithin and lecithin–cholesterol bilayers

K¹,

Yamaguchi²,

Inoko³

et al. 1976

Acta Crystallogr Sect B

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Dipalmitoyl lecithin and dipalmitoyl lecithin-cholesterol bilayers are capable of binding about the same number of uranyl ions as lecithin molecules. Dispersions of the uranyl-decorated bilayers give very distinct continuous X-ray diffraction peaks up to the seventh order. Electron density projection onto the normal to the membrane is obtained with a resolution of about 6.5 ~. It gives two high peaks separated by 48/~, indicating that uranyl ions are bound at the surfaces of membranes in both lecithin and lecithin--cholesterol bilayers.

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X-Ray Diffraction Studies of Lecithin Bimolecular Leaflets with Incorporated Fluorescent Probes

Cited by 127 publications

References 11 publications

Deuterium magnetic resonance: theory and application to lipid membranes

Deuterium magnetic resonance: theory and application to lipid membranes

1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane Dynamics?

Structures of uranyl-decorated lecithin and lecithin–cholesterol bilayers

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