X-ray diffraction study of structural stability of giant proteoglycan molecules of mucus

Instrumental and methodical approaches to the study of the transformation of nanostructural parameters in biological objects

“…After the HF action, the diffractograms of the welding junc tions of the intestine tissue in the small angle range are literally "filled" by sharp diffraction rings: in the nanosized range from 10 to 0.4 nm, a series of sharp Debye rings at the main spacing of 4.65 nm was recorded. The nanostructural invariant 4.65 (±0.15) nm was attributed by us earlier to the identity period, which is caused by the regular attachment of oli gosaccharide chains to the protein core of the giant molecule of proteoglycans of mucin and intercellular of tissue matrix of tissue [8]. The phenomenon of the appearance of new diffraction lines after HF electrosurgical action was also determined by us on the examples of the silk cocoon of the Bombyx mori silkworm: numerous thin Debye rings in the range from 5 to 0.5 nm were recorded, which are caused by nanostructured ordering, despite the molecular structure remaining stable.…”

“…The translation of object (3) is per formed with the use of positioner (8); this enables one to direct the X ray beam to the investigated part of sample.…”

“…The difference between functionally valuable structures, which appear on diffractogram as diffraction lines, is represented by the character and degree of their order ing, as well as the time parameters of their structural dynamics. Large scale X ray diffraction and spectral studies of biological objects became possible only due to the use of unique experimental procedures devel oped earlier based on SR [1][2][3][4][5][6][7][8][9]. Due to the unique characteristics, namely, high intensity, wide radiation spectrum, and coherence, SR allows one to obtain unique structural information on objects in a wide range of sizes from fractions of a nanometer to several centimeters.…”