X-ray fluorescent CT imaging of cerebral uptake of stable-iodine perfusion agent iodoamphetamine analog IMP in mice

Abstract

Using X-ray fluorescent computed tomography (XFCT), the in vivo and ex vivo cerebral distribution of a stable-iodine-labeled cerebral perfusion agent, iodoamphetamine analog (127I-IMP), has been recorded in the brains of mice. In vivo cerebral perfusion in the cortex, hippocampus and thalamus was depicted at 0.5 mm in-plane spatial resolution. Ex vivo XFCT images at 0.25 mm in-plane spatial resolution allowed the visualisation of the detailed structures of these regions. The quality of the XFCT image of the hippocampus was comparable with the 125I-IMP autoradiogram. These results highlight the sensitivity of XFCT and its considerable potential to evaluate cerebral perfusion in small animals without using radioactive agents.

Order of Magnitude Sensitivity Increase in X-ray Fluorescence Computed Tomography (XFCT) Imaging With an Optimized Spectro-Spatial Detector Configuration: Theory and Simulation

“…Simionovici et al demonstrated high-resolution X-ray fluorescence micro-tomography in 2000 [4]. In 2008, Takeda et al performed in vivo XFCT imaging of iodine perfusion in a mouse brain [5]. All of these experiments used mono-energetic X-rays from synchrotron radiation.…”

The potential of L-shell X-ray fluorescence CT (XFCT) for molecular imaging

“…Small beam size and fine scanning steps result in high spatial resolution down to 150 mm, with the expense of long imaging times. For example, the 20 mgAu ml 21 concentration measured with a single collimated detector by Manohar et al 5 required an imaging time of more than 8 h. Long imaging times are impractical for pre-clinical imaging, as duration of animal anaesthesia should not exceed 1.5 h. Takeda et al 6 were able to generate synchrotron pencil beam K-shell XFCT images of iodine in mice brains with imaging times of 1.5 h.…”

The feasibility study on 3-dimensional fluorescent x-ray computed tomography using the pinhole effect for biomedical applications

“…FXCT has been a vital tool in both material and biomedical sciences [4][5][6]. Molecular imaging using a non-radioactive imaging-agent, such as iodine, is also of potential use in medicine and pharmacology, where the visualization of various disease processes in small animals is used to investigate the cause, diagnosis and therapy of diseases from the characteristics and behavior of imaging agents in the subject.…”