X-ray imaging of tumor growth in live mice by detecting gold-nanoparticle-loaded cells

Chien, Chia‐Chi; Chen, Hsiang‐Hsin; Lai, Soon Onn; Hwu, Y.; Petibois, Cyril; Yang, Chu-Sing; Chu, Yong S.; Margaritondo, G.

doi:10.1038/srep00610

Cited by 33 publications

(19 citation statements)

References 39 publications

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“…It has been reported that 1.9 nm AuNP 47 and 15 nm AuNP coated with m-PEG did not produce any adverse effect in mice. 32 In vitro toxicity of 15 nm AuNP coated with 11-MUA, 33 HDL coated AuNP, 5 PEI capped 15 nm AuNP, 34 silica coated AuNP 16 and a 153 nm PEGylated gold nanoshells 48 are found to be cytocompatible in a range of cell lines and under a range of conditions. Another in vitro study reported that cetyltrimethylammonium bromide coated gold nanorods reduce the HeLa cell viability 21%, whereas for the case of polyelectrolytes (such as poly(diallyldimethylammonium chloride)–poly(4-styrenesulfonic acid) coated gold nanorods are cytocompatible.…”

Section: Discussionmentioning

confidence: 99%

Systematic in vitro toxicological screening of gold nanoparticles designed for nanomedicine applications

Naha

Chhour

Cormode

2015

Toxicology in Vitro

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Gold nanoparticles (AuNP) are increasingly being applied in the biomedical field as therapeutics, contrast agents, and in diagnostic systems, motivating investigations of their toxicity that might arise from accidental exposure. While other work has investigated the toxicological response to gold nanoparticles for industrial purposes, here we have surveyed formulations that have been developed for biomedical use, are in clinical trials or have been FDA-approved. The AuNP library tested contains a range of shapes, inculding spheres, rods and shells, that possess a range of coatings, such as silica, citrate, lipoprotein, polymaleic acid, polyethylene glycol, DNA and others. Good cytocompatibility for all formulations was observed after 1 hour of incubation. However after 24 hours exposure, a nanorod and a spherical DNA coated formulation resulted in toxicity. The coating material was the only factor that influenced toxicity. AuNP exposure seemed to have no effect on cell cytoskeleton deformation and cell spreading. Cell uptake, as measured by computed tomography and ICP-OES, as well as TEM images of cells, confirmed strong AuNP uptake for certain formulations, but there was no correlation with toxicity. No glove translocation occurred, therefore, nitrile gloves are an adequate safety precaution for working with the AuNP studied. In conclusion, the majority of AuNP formulations tested have very low adverse effects.

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Section: Discussionmentioning

confidence: 99%

Systematic in vitro toxicological screening of gold nanoparticles designed for nanomedicine applications

Naha

Chhour

Cormode

2015

Toxicology in Vitro

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“…Optical methods such as confocal microscopy, multi-photon microscopy (Maurin et al 2011) and optical coherence tomography (Vakoc et al 2012) have higher resolution but are limited to a relatively thin region closest to the surface. Very high-resolution imaging (<10 μm) of the microvasculature of mouse tumors using x-ray absorption-contrast agents has been demonstrated at synchrotrons (Chien et al 2012, Chien et al 2010, but the availability of these large facilities is limited and the required radiation dose using absorption contrast is incompatible with longitudinal studies.…”

Section: Introductionmentioning

confidence: 99%

X-ray phase contrast with injected gas for tumor microangiography

et al. 2014

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We show that the microvasculature of mouse tumors can be visualized using propagation-based phase-contrast x-ray imaging with gas as the contrast agent. The large density difference over the gas-tissue interface provides high contrast, allowing the imaging of small-diameter blood vessels with relatively short exposure times and low dose using a compact liquid-metal-jet x-ray source. The method investigated is applied to tumors (E1A/Ras-transformed mouse embryonic fibroblasts) grown in mouse ears, demonstrating sub-15-µm-diameter imaging of their blood vessels. The exposure time for a 2D projection image is a few seconds and a full tomographic 3D map takes some minutes. The method relies on the strength of the vasculature to withstand the gas pressure. Given that tumor vessels are known to be more fragile than normal vessels, we investigate the tolerance of the vasculature of 12 tumors to gas injection and find that a majority withstand 200 mbar pressures, enough to fill 12-µm-diameter vessels with gas. A comparison of the elasticity of tumorous and non-tumorous vessels supports the assumption of tumor vessels being more fragile. Finally, we conclude that the method has the potential to be extended to the imaging of 15 µm vessels in thick tissue, including mouse imaging, making it of interest for, e.g., angiogenesis research.

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“…Wang et al designed cationic surface modified GNPs with enhanced cellular uptake as a novel radio-sensitizer to lower the effective dose of X-ray in cancer radiation therapy [36]. Chien et al used GNPs as contrast agents for X-ray imaging of tumor growth in vivo based on the extinction effect of GNPs [37]. In this study,…”

Section: Discussionmentioning

confidence: 96%

Spider toxin peptide lycosin-i functionalized gold nanoparticles for in vivo tumor targeting and therapy

Tan

Huang

et al. 2019

Toxicon

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X-ray imaging of tumor growth in live mice by detecting gold-nanoparticle-loaded cells

Cited by 33 publications

References 39 publications

Systematic in vitro toxicological screening of gold nanoparticles designed for nanomedicine applications

Systematic in vitro toxicological screening of gold nanoparticles designed for nanomedicine applications

X-ray phase contrast with injected gas for tumor microangiography

Spider toxin peptide lycosin-i functionalized gold nanoparticles for in vivo tumor targeting and therapy

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