X-ray imaging optimization of 3D tissue engineering scaffolds via combinatorial fabrication methods

Yang, Yanyin; Dorsey, Shauna M.; Becker, Matthew L.; Lin‐Gibson, Sheng; Schumacher, Gary E.; Flaim, Glenn M.; Kohn, Joachim; Simon, Carl G.

doi:10.1016/j.biomaterials.2007.12.042

Cited by 35 publications

(30 citation statements)

References 31 publications

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“…[ 55 ] X-ray radiography and X-ray microcomputed tomography enable non-invasive imaging of implants in vivo and in vitro. However, polymeric materials, such as highly porous scaffolds, generally do not possess sufficient X-ray contrast and are therefore diffi cult to image with X-ray-based techniques.…”

Section: Pdtec/pi 2 Dtec Scaffold Library Gradientsmentioning

confidence: 99%

Combinatorial and High‐Throughput Screening of Biomaterials

2010

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Combinatorial and high-throughput methods have been increasingly used to accelerate research and development of new biomaterials. These methods involve creating miniaturized libraries that contain many specimens in one sample in the form of gradients or arrays, followed by automated data collection and analysis. This article reviews recent advances in utilizing combinatorial and high-throughput methods to better understand cell-material interactions, particularly highlighting our efforts at the NIST Polymers Division. Specifically, fabrication techniques to generate controlled surfaces (2D) and 3D cell environments (tissue engineering scaffolds) as well as methods to characterize and analyze material properties and cell-material interactions are described. In conclusion, additional opportunities for combinatorial methods for biomaterials research are noted, including streamlined sample fabrication and characterization, appropriate and automated bioassays, and data analysis.

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Section: Pdtec/pi 2 Dtec Scaffold Library Gradientsmentioning

confidence: 99%

Combinatorial and High‐Throughput Screening of Biomaterials

2010

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“…Freeze-drying to remove the solvent and dissolution of the salt resulted in a porous monolith (roughly 1 cm × 1 cm × 5 cm in dimension) that gradually changed in its composition from one end to the other. A first application of this technique resulted in the identification of the optimal level of iodinated polymer additive needed to create contrast in X-ray imaging measurements (e.g., X-ray tomography) of polymer tissue scaffolds [67]. In this study, the gradient polymer blend composition library was fabricated so that it gradually increased in the level of iodinated species along its length.…”

Section: Polymer Blend Composition Gradientsmentioning

confidence: 99%

Gradient and Microfluidic Library Approaches to Polymer Interfaces

Fasolka¹,

Stafford²,

Beers³

2009

Polymer Libraries

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We present an overview of research conducted at the National Institute of Standards and Technology aimed at developing and applying combinatorial and high-throughput measurement approaches to polymer surfaces, interfaces and thin films. Topics include (1) the generation of continuous gradient techniques for fabricating combinatorial libraries of film thickness, temperature, surface chemistry and polymer blend composition, (2) high-throughput measurement techniques for assessing the mechanical properties and adhesion of surfaces, interfaces and films, and (3) microfluidic approaches to synthesizing and analyzing libraries of interfaciallyactive polymer species.

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“…For example, X-ray imaging and corresponding micro computed tomography (microCT) have been optimized for imaging engineered tissue scaffolds using a combinatorial method to minimize the use of contrast agents [13]. Magnetic resonance imaging (MRI) has been used to monitor mesenchymal stem cell differentiation in tissue engineering [14].…”

Section: Introductionmentioning

confidence: 99%

Imaging engineered tissues using structural and functional optical coherence tomography

Lü

Graf

Boppart³

2009

Journal of Biophotonics

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As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significant advantages to visualize these changes. Structural OCT has been used to investigate the longitudinal development of engineered tissues and cell dynamics such as migration, proliferation, detachment, and cell-material interactions. Optical techniques that image functional parameters or integrate multiple imaging modalities to provide complementary contrast mechanisms have been developed, such as the integration of optical coherence microscopy with multiphoton microscopy to image structural and functional information from cells in engineered tissue, optical coherence elastography to generate images or maps of strain to reflect the spatially-dependent biomechanical properties, and spectroscopic OCT to differentiate different cell types. From these results, OCT demonstrates great promise for imaging and visualizing engineered tissues, and the complex cellular dynamics that directly affect their practical and clinical use.

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X-ray imaging optimization of 3D tissue engineering scaffolds via combinatorial fabrication methods

Cited by 35 publications

References 31 publications

Combinatorial and High‐Throughput Screening of Biomaterials

Combinatorial and High‐Throughput Screening of Biomaterials

Gradient and Microfluidic Library Approaches to Polymer Interfaces

Imaging engineered tissues using structural and functional optical coherence tomography

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