X-ray mosaic nanotomography of large microorganisms

Quaroni, Luca; Marone, Federica; Irvine, Sarah; Vila‐Comamala, Joan; Blanke, Alexander; Stampanoni, Marco

doi:10.1016/j.jsb.2011.12.014

Cited by 21 publications

(19 citation statements)

References 27 publications

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“…The reason for the FOVs being different is that the ultrastructural elements need to be visually identified, which relies on high spatial resolutions, which in turn limit the FOV, and, consequently, the size of the area/ volume that can be investigated. One way to overcome this limitation and to extend the effective FOV is through imaging adjacent sample areas [181].…”

Section: Imaging Techniquesmentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

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Bone's remarkable mechanical properties are a result of its hierarchical structure. The mineralized collagen fibrils, made up of collagen fibrils and crystal platelets, are bone's building blocks at an ultrastructural level. The organization of bone's ultrastructure with respect to the orientation and arrangement of mineralized collagen fibrils has been the matter of numerous studies based on a variety of imaging techniques in the past decades. These techniques either exploit physical principles, such as polarization, diffraction or scattering to examine bone ultrastructure orientation and arrangement, or directly image the fibrils at the sub-micrometre scale. They make use of diverse probes such as visible light, X-rays and electrons at different scales, from centimetres down to nanometres. They allow imaging of bone sections or surfaces in two dimensions or investigating bone tissue truly in three dimensions, in vivo or ex vivo, and sometimes in combination with in situ mechanical experiments. The purpose of this review is to summarize and discuss this broad range of imaging techniques and the different modalities of their use, in order to discuss their advantages and limitations for the assessment of bone ultrastructure organization with respect to the orientation and arrangement of mineralized collagen fibrils.

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Section: Imaging Techniquesmentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

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“…Full-field X-ray imaging is a valuable tool lying on the border of optical and electron microscopy. It allows access to 3D structural details of biological systems at the nanoscale, while keeping the sample close to the natural state (Mokso et al, 2012;Parkinson et al, 2013).…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Tripolar mitosis in human cells and embryos: Occurrence, pathophysiology and medical implications

Kalatova¹,

Jesenska

Hlinka³

et al. 2015

Acta Histochemica

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Tripolar mitosis is a specific case of cell division driven by typical molecular mechanisms of mitosis, but resulting in three daughter cells instead of the usual count of two. Other variants of multipolar mitosis show even more mitotic poles and are relatively rare. In nature, this phenomenon was frequently observed or suspected in multiple common cancers, infected cells, the placenta, and in early human embryos with impaired pregnancy-yielding potential. Artificial causes include radiation and various toxins. Here we combine several pieces of the most recent evidence for the existence of different types of multipolar mitosis in preimplantation embryos together with a detailed review of the literature. The related molecular and cellular mechanisms are discussed, including the regulation of centriole duplication, mitotic spindle biology, centromere functions, cell cycle checkpoints, mitotic autocorrection mechanisms, and the related complicating factors in healthy and affected cells, including post-mitotic cell-cell fusion often associated with multipolar cell division. Clinical relevance for oncology and embryo selection in assisted reproduction is also briefly discussed in this context.

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“…After taking the subsample, the original sample is modified, which can be undesirable in some cases. Several methods exist to extend the reconstructed area (Pfeiffer, 2004;Zamyatin et al, 2005), to reconstruct horizontally truncated data (Defrise et al, 2006;Vlassenbroeck, 2009) or to overcome the limitation by mosaic scanning (Haberthür et al, 2010;Mokso et al, 2012), but the link between sample size and voxel size remains. Particularly in geosciences, where heterogeneity is an important characteristic, multi-scale imaging is gaining importance.…”

Section: Non-destructive Techniquementioning

confidence: 99%

High-resolution X-ray computed tomography in geosciences: A review of the current technology and applications

Cnudde

Boone

2013

Earth-Science Reviews

1,263

700

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Please cite this article as: Cnudde, V., Boone, M.N., "High-resolution X-ray computed tomography in geosciences: a review of the current technology and applications", Earth Science Reviews (2013Reviews ( ), doi: 10.1016Reviews ( /j.earscirev.2013 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A C C E P T E D M A N U S C R I P T AbstractHigh-resolution X-ray Computed Tomography (HRXCT) or micro-CT (µCT) is a frequently used non-destructive 3D imaging and analysis technique for the investigation of internal structures of a large variety of objects, including geomaterials. Although the possibilities of X-ray micro-CT are becoming better appreciated in earth science research, the demands on this technique are also approaching certain physical limitations. As such, there remains a lot of research to be done in order to solve all the technical problems that occur when higher demands are put on the technique. In this paper, a review of the principle, the advantages and limitations of X-ray CT itself are presented, together with an overview of some current applications of micro-CT in geosciences. One of the main advantages of this technique is the fact that it is a non-destructive characterization technique which allows 4D monitoring of internal structural changes at resolutions down to a few hundred nanometers. Limitations of this technique are the operator dependency for the 3D image analysis from the reconstructed data, the discretations effects and possible imaging artefacts. Driven by the technological and computational progress, the technique is continuously growing as an analysis tool in geosciences and is becoming one of the standard techniques, as is shown by the large and still increasing number of publications in this research area. It is foreseen that this number will continue to rise, and micro-CT will become an indispensable technique in the field of geosciences.

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X-ray mosaic nanotomography of large microorganisms

Cited by 21 publications

References 27 publications

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Tripolar mitosis in human cells and embryos: Occurrence, pathophysiology and medical implications

High-resolution X-ray computed tomography in geosciences: A review of the current technology and applications

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