X-ray sterilization of the An. arabiensis genetic sexing strain ‘ANO IPCL1’ at pupal and adult stages

Ndo, Cyrille; Yamada, Hanano; Damiens, David; N'Do, Sévérin; Seballos, G.; Gilles, Jérémie

doi:10.1016/j.actatropica.2013.11.027

Cited by 20 publications

(23 citation statements)

References 16 publications

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“…A lower dose of ionizing radiation generally means less somatic damage, and therefore more competitive released males. This approach termed as the "Combi-Fly concept" (Ndo et al, 2013;Helinski et al, 2006) was already earlier suggested for fruit fly control as part of an IPM programme in which the release of a semi-sterile strain would induce a higher level of sterility in the target population due to their increased competitiveness compared to the fully sterilized alternative (Franz, 2000;Steffens, 1982). ANO IPCL1 males that are not irradiated were more sterile than males from the wild-type Dongola strain that were irradiated with 75 Gy.…”

Section: Using the Ano Ipcl1mentioning

confidence: 99%

The Anopheles arabiensis genetic sexing strain ANO IPCL1 and its application potential for the sterile insect technique in integrated vector management programmes

et al. 2015

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The Anopheles arabiensis genetic sexing strain ANO IPCL1 was developed based on a dieldrin resistant mutation. The strain has been shown to be practical and reliable in terms of female elimination by dieldrin treatments at larval stages, but has provided some difficulties when treatments were applied at the egg stage. The high natural sterility of this strain has advantages and disadvantages in both mass rearing and the sterilization process. In addition, its recombination rate, although relatively low, poses a threat of strain deterioration if left unchecked in a mass-rearing setting. The males of the ANO IPCL1 have been shown to be equally competitive as lab-reared males of the wild-type Dongola strain, but competitiveness decreased by half when irradiated with 75 Gy—a dose conferring >98% sterility. More controversial issues surround the use of dieldrin—a highly persistent organochlorine that is known to bioaccumulate in the food chain. The prospective use of large volumes of dieldrin in a mass-rearing facility and the retention of its residues by the male mosquitoes makes the use of the strain in the context of the sterile insect technique against this vector highly questionable, and therefore its implementation at a large scale cannot be recommended.

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Section: Using the Ano Ipcl1mentioning

confidence: 99%

The Anopheles arabiensis genetic sexing strain ANO IPCL1 and its application potential for the sterile insect technique in integrated vector management programmes

et al. 2015

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“…In recent experiments assessing the effects of X-ray irradiation on the genetic sexing strain of An. arabiensis "ANO IPCL1" (NDO et al 2013), all treatment groups showed slightly decreased longevity; which may be the result of stress induced by handling procedures or the stackable plates in which the pupae are held for the duration of the irradiation process. Currently, the pupae lay on their sides in shallow water.…”

Section: Discussionmentioning

confidence: 91%

X-Ray-Induced Sterility inAedes albopictus(Diptera: Culicidae) and Male Longevity Following Irradiation

et al. 2014

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The mosquito Aedes albopictus (Skuse, 1895) is a potent vector of several arboviral diseases, most notably chikungunya and dengue fever. In the context of the sterile insect technique (SIT), the sterilization of the male mosquitoes before their release can be achieved by gamma-ray irradiation. As gamma-ray irradiators are becoming increasingly problematic to purchase and transport, the suitability of an X-ray irradiator as an alternative for the sterilization of Ae. albopictus males was studied. The sterilization of up to 200,000 pupae at one time can be achieved with relative ease, and the sterility results obtained were comparable with those achieved by gamma irradiation, where 99% sterility is induced with a dose of 40 Gy. A significant reduction of longevity was observed in the latter stages of the males' life after irradiation treatments, especially at doses > 40 Gy, which is consistent with the negative effects on longevity induced by similar radiation doses using gamma rays. Females irradiated at 40 Gy were not only 100% sterile, but also failed to oviposit entirely, i.e., all of the females laid 0 eggs. Overall, it was found that the X-ray irradiator is generally suitable for the sterilization process for sterile insect technique programs, as it showed a high processing capacity, practicality, high effectiveness, and reproducibility.

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“…The ability to eliminate females at this early developmental stage has advantages, such as reduced production costs, labour and space requirements [7], but may impact the biology or performance of the treated adults. Finally, the male pupae are subjected to a dose of 75 Gy of gamma or X-ray irradiation resulting in >98% sterility [8] before they emerge and are ready for release.…”

Section: Introductionmentioning

confidence: 99%

“…The GSS ANO IPCL1 has been evaluated in terms of reliability of female elimination [7], rearing parameters [9], strain management [7], and radiation sensitivity [7, 8]. GSSs based on dieldrin resistance have been produced in the past for An.…”

Section: Introductionmentioning

confidence: 99%

“…arabiensis with gamma rays (with doses ranging from 25–100 Gy) had little impact on pupal survival, adult longevity and fecundity of females mated with irradiated males [18]. Sterilizing male mosquitoes at the adult and pupal stages using X-rays (at doses ranging from 35–105 Gy) had some negative effects on adult longevity, possibly due to the stress imposed on them while in the irradiation canister, as well as somatic damage caused by irradiation [8]. Moreover, X-ray irradiation at 70 Gy decreased the number of sperm in testis in two-day old males and inhibited subsequent sperm production during adult life [12].…”

Section: Introductionmentioning

confidence: 99%

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The effects of genetic manipulation, dieldrin treatment and irradiation on the mating competitiveness of male Anopheles arabiensis in field cages

Yamada

Vreysen

Gilles

et al. 2014

Malar J

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BackgroundTo enable the release of only sterile male Anopheles arabiensis mosquitoes for the sterile insect technique, the genetic background of a wild-type strain was modified to create a genetic sexing strain ANO IPCL1 that was based on a dieldrin resistance mutation. Secondly, the eggs of ANO IPCL1 require treatment with dieldrin to allow complete elimination of female L1 larvae from the production line. Finally, male mosquito pupae need to be treated with an irradiation dose of 75 Gy for sterilization. The effects of these treatments on the competitiveness of male An. arabiensis were studied.MethodsThe competitiveness of ANO IPCL1 males that were treated either with irradiation or both dieldrin and irradiation, was compared with that of the wild-type strain (An. arabiensis Dongola) at a 1:1 ratio in 5.36 m3 semi-field cages located in a climate-controlled greenhouse. In addition, three irradiated: untreated male ratios were tested in semi-field cages (1:1, 5:1 and 10:1) and their competition for virgin wild-type females was assessed.ResultsThe ANO IPCL1 males were equally competitive as the wild-type males in this semi-field setting. The ANO IPCL1 males irradiated at 75 Gy were approximately half as competitive as the unirradiated wild-type males. ANO IPCL1 males that had been treated with dieldrin as eggs, and irradiated with 75 Gy as pupae were slightly more competitive than males that were only irradiated. Ratios of 1:1, 5:1 and 10:1 irradiated ANO IPCL1 males: untreated wild-type males resulted in 31, 66 and 81% induced sterility in the female cage population, respectively.ConclusionsAn irradiation dose of 75 Gy reduced the competitiveness of male ANO IPCL1 significantly and will need to be compensated by releasing higher numbers of sterile males in the field. However, the dieldrin treatment used to eliminate females appears to have an unexpected radioprotectant effect, however the mechanism is not understood. A sterile to wild-type ratio of 10:1 effectively reduced the population’s fertility under the experimental field cage conditions, but further studies in the field will be needed to confirm the efficiency of sterile ANO IPCL1 males when competing against wild males for wild females.

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X-ray sterilization of the An. arabiensis genetic sexing strain ‘ANO IPCL1’ at pupal and adult stages

Cited by 20 publications

References 16 publications

The Anopheles arabiensis genetic sexing strain ANO IPCL1 and its application potential for the sterile insect technique in integrated vector management programmes

The Anopheles arabiensis genetic sexing strain ANO IPCL1 and its application potential for the sterile insect technique in integrated vector management programmes

X-Ray-Induced Sterility inAedes albopictus(Diptera: Culicidae) and Male Longevity Following Irradiation

The effects of genetic manipulation, dieldrin treatment and irradiation on the mating competitiveness of male Anopheles arabiensis in field cages

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